Morphophysiological analysis of the salivary glands of Rhipicephalus sanguineus sensu lato ( Acari : Ixodidae ) exposed to ozonated water: A control strategy

2020 ◽  
Author(s):  
M. R. Abreu ◽  
I. Delalibera ◽  
N. R. C. Pereira ◽  
M. I. Camargo‐Mathias
Keyword(s):  
Salivary Glands ◽  
Control Strategy ◽  
Rhipicephalus Sanguineus ◽  
Rhipicephalus Sanguineus Sensu Lato ◽  
Ozonated Water

Thymol action on cells and tissues of the synganglia and salivary glands of Rhipicephalus sanguineus sensu lato females (Acari: Ixodidae)

2019 ◽  
Vol 10 (2) ◽  
pp. 314-320 ◽  
Author(s):  
Renata Silva Matos ◽  
Erik Daemon ◽  
Caio Márcio de Oliveira Monteiro ◽  
Bruno Rodrigues Sampieri ◽  
Paula Barroso C. Marchesini ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Rhipicephalus Sanguineus ◽  
Rhipicephalus Sanguineus Sensu Lato

scholarly journals Investigation of Transovarial Transmission of Bartonella henselae in Rhipicephalus sanguineus sensu lato Ticks Using Artificial Feeding

2021 ◽  
Vol 9 (12) ◽  
pp. 2501
Author(s):  
Wittawat Wechtaisong ◽  
Sarah I. Bonnet ◽  
Bruno B. Chomel ◽  
Yi-Yang Lien ◽  
Shih-Te Chuang ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Nested Pcr ◽  
Bartonella Henselae ◽  
Rhipicephalus Sanguineus ◽  
Artificial Feeding ◽  
Transovarial Transmission ◽  
Larval Feeding ◽  
Infected Goat ◽  
Rhipicephalus Sanguineus Sensu Lato ◽  
Slow Growing

Bartonella henselae is a slow-growing, Gram-negative bacterium that causes cat scratch disease in humans. A transstadial transmission of the bacteria from larvae to nymphs of Rhipicephalus sanguineus sensu lato (s.l.) ticks, suspected to be a potential vector of the bacteria, has been previously demonstrated. The present study aims to investigate transovarial transmission of B. henselae from R. sanguineus s.l. adults to their instars. Adult ticks (25 males and 25 females) were fed through an artificial feeding system on B. henselae-infected goat blood for 14 days, and 300 larvae derived from the experimentally B. henselae-infected females were fed on noninfected goat blood for 7 days. Nested PCR and culture were used to detect and isolate B. henselae in ticks and blood samples. Bartonella henselae DNA was detected in midguts, salivary glands, and carcasses of the semi-engorged adults and pooled tick feces (during feeding and post-feeding periods). After the oviposition period, B. henselae DNA was detected in salivary glands of females (33.3%), but not in pooled eggs or larvae derived from the infected females. However, B. henselae DNA was detected by nested PCR from the blood sample during larval feeding, while no viable B. henselae was isolated by culture. According to our findings, following infected blood meal, B. henselae could remain in the tick midguts, move to other tissues including salivary glands, and then be shed through tick feces with limited persistency. The presence of bacterial DNA in the blood during larval feeding shows the possibility of transovarial transmission of B. henselae in R. sanguineus s.l. ticks.

Exposure of Ticks Rhipicephalus sanguineus sensu lato (Acari:Ixodidae) to Ozonated Water: Integument Response

2020 ◽  
Vol 42 (6) ◽  
pp. 507-516 ◽  
Author(s):  
Marina Rodrigues de Abreu ◽  
Italo Delalibera Junior ◽  
Natalia Rubio Claret Pereira ◽  
Maria Izabel Camargo-Mathias
Keyword(s):  
Rhipicephalus Sanguineus ◽  
Rhipicephalus Sanguineus Sensu Lato ◽  
Ozonated Water

scholarly journals Comparative Proteomic Analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) Tropical and Temperate Lineages: Uncovering Differences During Ehrlichia canis Infection

2021 ◽  
Vol 10 ◽  
Author(s):  
Gustavo Seron Sanches ◽  
Margarita Villar ◽  
Joana Couto ◽  
Joana Ferrolho ◽  
Isabel G. Fernández de Mera ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Vector Competence ◽  
Rhipicephalus Sanguineus ◽  
Ehrlichia Canis ◽  
Biological Traits ◽  
Tick Feeding ◽  
Pathogen Infection ◽  
Rhipicephalus Sanguineus Sensu Lato ◽  
Tick Borne Disease ◽  
Morphological Differences

The tick vector Rhipicephalus sanguineus is established as a complex of closely related species with high veterinary-medical significance, in which the presence of different genetic, morphological, and biological traits has resulted in the recognition of different lineages within taxa. One of the most striking differences in the “temperate” and “tropical” lineages of R. sanguineus (s.l.) is the vector competence to Ehrlichia canis, suggesting that these ticks tolerate and react differently to pathogen infection. The present study addresses the SG and MG proteome of the R. sanguineus tropical and temperate lineages and compares their proteomic profile during E. canis infection. Batches of nymphs from the two lineages were allowed to feed on naïve and experimentally E. canis infected dogs and after molting, adults were dissected, and salivary glands and midgut tissues separated. Samples were screened for the presence of E. canis before proteomic analyses. The representation of the proteins identified in infected and non-infected tissues of each lineage was compared and gene ontology used for protein classification. Results highlight important differences in those proteomic profiles that added to previous reported genetic, biological, behavioral, and morphological differences, strengthening the hypothesis of the existence of two different species. Comparing infected and non-infected tissues, the results show that, while in midgut tissues the response to E. canis infection is similar in the salivary glands, the two lineages show a different pattern of protein representation. Focusing on the proteins found only in the infected condition, the data suggests that the cement cone produced during tick feeding may be implicated in pathogen infection. This study adds useful information to the debate on the controversial R. sanguineus systematic status, to the discussion related with the different vectorial competence occurring between the two lineages and identifies potential targets for efficient tick and tick-borne disease control.

scholarly journals Successful isolation of Leishmania infantum from Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) collected from naturally infected dogs

2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Viviane Medeiros-Silva ◽  
Rodrigo Gurgel-Gonçalves ◽  
Nadjar Nitz ◽  
Lucia Emilia D’ Anduraim Morales ◽  
Laurício Monteiro Cruz ◽  
...  
Keyword(s):  
Leishmania Infantum ◽  
Rhipicephalus Sanguineus ◽  
Successful Isolation ◽  
Rhipicephalus Sanguineus Sensu Lato

Efficacy of collars with allomones on dogs to control Rhipicephalus sanguineus sensu lato infestations under field conditions

2021 ◽  
pp. 101769
Author(s):  
Lucas P. Barreto ◽  
Cárita S. Ribeiro-Silva ◽  
Lucas Damião C. Machado ◽  
Valesca H. Lima ◽  
Salorrane M.N. Pinto ◽  
...  
Keyword(s):  
Rhipicephalus Sanguineus ◽  
Field Conditions ◽  
Rhipicephalus Sanguineus Sensu Lato
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