Hemalin from Haemaphysalis flava ticks: cloning, expression and antithrombogenicity

2020 ◽  
Author(s):  
L. Liu ◽  
H. Tang ◽  
L.‐L. Feng ◽  
T.‐Y. Cheng
Keyword(s):  
Haemaphysalis Flava

scholarly journals Comparative population genetic structure of two Ixodidae ticks (Ixodes ovatus and Haemaphysalis flava) in Niigata Prefecture, Japan

2019 ◽  
Author(s):  
Maria Angenica Fulo Regilme ◽  
Megumi Sato ◽  
Tsutomu Tamura ◽  
Reiko Arai ◽  
Marcello Otake Sato ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Large Scale ◽  
Southern China ◽  
Isolation By Distance ◽  
Mantel Test ◽  
Rrna Gene ◽  
Data Set ◽  
Genetic Groups ◽  
Niigata Prefecture ◽  
Haemaphysalis Flava

AbstractIxodid tick species such as Ixodes ovatus and Haemaphysalis flava are important vector of tick-borne diseases in Japan. In this study, we used genetic structure at two mitochondrial loci (cox1, 16S rRNA gene) to infer gene flow patterns of I. ovatus and H. flava from Niigata Prefecture, Japan. Samples were collected in 29 (I. ovatus) and 17 (H. flava) sampling locations across Niigata Prefecture (12,584.18 km2). For I. ovatus, pairwise FST and analysis of molecular variance (AMOVA) analyses of cox1 sequences indicated significant among-population differentiation. This was in contrast to H. flava, for which there were few cases of low significant pairwise differentiation. A Mantel test revealed isolation by distance and there was positive spatial autocorrelation of haplotypes in I. ovatus cox1 and 16S sequences, but non-significant results were observed in H. flava in both markers. We found three genetic groups (China 1, China 2 and Japan) in the cox1 I. ovatus tree. Newly sampled I. ovatus grouped together with a published I. ovatus sequence from northern Japan and were distinct from two other I. ovatus groups that were reported from southern China. The three genetic groups in our data set suggest the potential for cryptic species among the groups. While many factors can potentially account for the observed differences in genetic structure between the two species, including population persistence and large-scale patterns of range expansion, the differences in the mobility of hosts of tick immature stages (small mammals in I. ovatus; birds in H. flava) is possibly driving the observed patterns.

Cathepsin L—a novel cysteine protease from Haemaphysalis flava Neumann, 1897

2019 ◽  
Vol 118 (5) ◽  
pp. 1581-1592 ◽  
Author(s):  
Yali Sun ◽  
Lan He ◽  
Long Yu ◽  
Jiaying Guo ◽  
Zheng Nie ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Cathepsin L ◽  
Haemaphysalis Flava

scholarly journals Tick surveillance in two southeastern provinces, including three metropolitan areas, of the Republic of Korea during 2014 

2017 ◽  
Vol 22 (2) ◽  
pp. 271 ◽  
Author(s):  
Jaree L. Johnson ◽  
Heung-chul Kim ◽  
Jordan M. Coburn ◽  
Sung-tae Chong ◽  
Nicholas W. Chang ◽  
...  
Keyword(s):  
Disease Surveillance ◽  
Metropolitan Areas ◽  
Republic Of Korea ◽  
Haemaphysalis Longicornis ◽  
Haemaphysalis Flava ◽  
Tick Borne Disease ◽  
Males And Females ◽  
The Mean ◽  
The Republic ◽  
Amblyomma Testudinarium

Tick-borne disease surveillance was conducted from March–October 2014 in two southeastern provinces, including three metropolitan areas, in the Republic of Korea (ROK). Three general habitats were surveyed: Grasses (grasses and herbaceous and crawling vegetation), Forests (pine, larch, deciduous, and mixed), and Forests+Grasses. A total of 40,048 ticks (1,480 adults; 24,201 nymphs; 14,367 larvae) belonging to three genera and six species were collected. Haemaphysalis longicornis (84.25%; 33,741) was the most commonly collected tick, followed by Haemaphysalis flava (14.52%; 5,816), Ixodes nipponensis (1.09%; 436), Amblyomma testudinarium (0.07%; 27), Haemaphysalis phasiana (0.06%; 23), and Ixodes turdus (0.01%; 5). Overall, adult ticks accounted for only 3.70% of all ticks collected, while nymphs and larvae accounted for 60.43% and 35.87%, respectively. The proportion of H. longicornis nymphs was highest beginning in March (99.51%), slowly declined through July (82.01%) and then rapidly declined to a low in October (6.45%). Large increases in the proportion of H. longicornis larvae were observed in August (42.05%), September (84.19%) and October (93.55%) following increased numbers of adults collected in June (4.20%), July (17.99%) and August (9.79%). Haemaphysalis flava adults and nymphs were commonly collected from April–May and October, while larvae were first collected from July, with peak numbers collected in August and low numbers collected during September–October. The proportion of I. nipponensis adults was highest in March (75.34%), declined to a low in July (0%), and then increased in September (60.00%) and October (90.32%). Larvae were collected only in August–September and accounted for 64.29% and 20.00% of all I. nipponensis collected during those months. Similar proportions of males and females of H. flava (51.47% and 48.53%, respectively) were collected from all habitats, while significantly more I. nipponensis males (62.20%) were collected than females (37.80%). Conversely, the proportion of H. longicornis females (80.00%) collected was significantly higher than for males (20.00%). Overall, the mean number of ticks collected/hr of collection for all habitats was 156.06/hr of collection. Similar numbers were collected/hr for Forests (172.61) and Grasses (168.64), while lower numbers were collected/hr for Forests+Grasses (128.12). 

Isolation and characterization of Kabuto Mountain virus, a new tick-borne phlebovirus from Haemaphysalis flava ticks in Japan

2018 ◽  
Vol 244 ◽  
pp. 252-261 ◽  
Author(s):  
Hiroko Ejiri ◽  
Chang-Kweng Lim ◽  
Haruhiko Isawa ◽  
Yukie Yamaguchi ◽  
Ryosuke Fujita ◽  
...  
Keyword(s):  
Isolation And Characterization ◽  
Haemaphysalis Flava

Genetic variation in mitochondrial genes of the tick Haemaphysalis flava collected from wild hedgehogs in China

2017 ◽  
Vol 71 (2) ◽  
pp. 131-137 ◽  
Author(s):  
Zhong-Bo Li ◽  
Tian-Yin Cheng ◽  
Xing-Li Xu ◽  
Lu-Lin Song ◽  
Guo-Hua Liu
Keyword(s):  
Genetic Variation ◽  
Mitochondrial Genes ◽  
Haemaphysalis Flava
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