Indoor and outdoor winter activity of Culicoides biting midges, vectors of bluetongue virus, in Italy

2017 ◽  
Vol 32 (1) ◽  
pp. 70-77 ◽  
Author(s):  
A. MAGLIANO ◽  
P. SCARAMOZZINO ◽  
S. RAVAGNAN ◽  
F. MONTARSI ◽  
G. DA ROLD ◽  
...  
Keyword(s):  
Bluetongue Virus ◽  
Biting Midges ◽  
Winter Activity ◽  
Culicoides Biting Midges ◽  
Indoor And Outdoor

scholarly journals Measurement of the Infection and Dissemination of Bluetongue Virus in Culicoides Biting Midges Using a Semi-Quantitative RT-PCR Assay and Isolation of Infectious Virus

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e70800 ◽  
Author(s):  
Eva Veronesi ◽  
Frank Antony ◽  
Simon Gubbins ◽  
Nick Golding ◽  
Alison Blackwell ◽  
...  
Keyword(s):  
Bluetongue Virus ◽  
Infectious Virus ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Biting Midges ◽  
Culicoides Biting Midges

scholarly journals An Early Block in the Replication of the Atypical Bluetongue Virus Serotype 26 in Culicoides Cells Is Determined by Its Capsid Proteins

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 919
Author(s):  
Marc Guimerà Busquets ◽  
Gillian D. Pullinger ◽  
Karin E. Darpel ◽  
Lyndsay Cooke ◽  
Stuart Armstrong ◽  
...  
Keyword(s):  
Bluetongue Virus ◽  
Mammalian Cells ◽  
Reverse Genetics ◽  
Reassortant Virus ◽  
Biting Midges ◽  
Potential Barriers ◽  
Virus Serotype ◽  
Vertebrate Hosts ◽  
Viral Determinants ◽  
Culicoides Biting Midges

Arboviruses such as bluetongue virus (BTV) replicate in arthropod vectors involved in their transmission between susceptible vertebrate-hosts. The “classical” BTV strains infect and replicate effectively in cells of their insect-vectors (Culicoides biting-midges), as well as in those of their mammalian-hosts (ruminants). However, in the last decade, some “atypical” BTV strains, belonging to additional serotypes (e.g., BTV-26), have been found to replicate efficiently only in mammalian cells, while their replication is severely restricted in Culicoides cells. Importantly, there is evidence that these atypical BTV are transmitted by direct-contact between their mammalian hosts. Here, the viral determinants and mechanisms restricting viral replication in Culicoides were investigated using a classical BTV-1, an “atypical” BTV-26 and a BTV-1/BTV-26 reassortant virus, derived by reverse genetics. Viruses containing the capsid of BTV-26 showed a reduced ability to attach to Culicoides cells, blocking early steps of the replication cycle, while attachment and replication in mammalian cells was not restricted. The replication of BTV-26 was also severely reduced in other arthropod cells, derived from mosquitoes or ticks. The data presented identifies mechanisms and potential barriers to infection and transmission by the newly emerged “atypical” BTV strains in Culicoides.

scholarly journals The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 230
Author(s):  
Marie Pourcelot ◽  
Rayane Amaral Moraes ◽  
Aurore Fablet ◽  
Emmanuel Bréard ◽  
Corinne Sailleau ◽  
...  
Keyword(s):  
Bluetongue Virus ◽  
Type I Interferon ◽  
Type I ◽  
Adapter Protein ◽  
Structural Protein ◽  
Innate Response ◽  
Biting Midges ◽  
Domestic Ruminants ◽  
Infected Cells ◽  
Culicoides Biting Midges

Bluetongue virus (BTV), an arbovirus transmitted by Culicoides biting midges, is a major concern of wild and domestic ruminants. While BTV induces type I interferon (alpha/beta interferon [IFN-α/β]) production in infected cells, several reports have described evasion strategies elaborated by this virus to dampen this intrinsic, innate response. In the present study, we suggest that BTV VP3 is a new viral antagonist of the IFN-β synthesis. Indeed, using split luciferase and coprecipitation assays, we report an interaction between VP3 and both the mitochondrial adapter protein MAVS and the IRF3-kinase IKKε. Overall, this study describes a putative role for the BTV structural protein VP3 in the control of the antiviral response.

DNA barcode identification and molecular detection of bluetongue virus in Culicoides biting midges (Diptera: Ceratopogonidae) from western Thailand

Acta Tropica ◽  
2021 ◽  
pp. 106147
Author(s):  
Yuki Fujisawa ◽  
Thanyaporn Homat ◽  
Arunrat Thepparat ◽  
Tanasak Changbunjong ◽  
Kripitch Sutummaporn ◽  
...  
Keyword(s):  
Bluetongue Virus ◽  
Molecular Detection ◽  
Dna Barcode ◽  
Biting Midges ◽  
Culicoides Biting Midges

scholarly journals Retrospective Analysis of the Bluetongue Outbreak in Serbia

2017 ◽  
Vol 40 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Spomenka Djurić ◽  
Predrag Simeunović ◽  
Milorad Mirilović ◽  
Jevrosima Stevanović ◽  
Uroš Glavinić ◽  
...  
Keyword(s):  
Bluetongue Virus ◽  
Control Measure ◽  
Infected Sheep ◽  
Economic Losses ◽  
Biting Midges ◽  
Domestic Ruminants ◽  
Investigation Period ◽  
Bluetongue Disease ◽  
Culicoides Biting Midges ◽  
Veterinary Institute

Abstract Bluetongue, a vector-born disease caused by the Bluetongue virus (BTV) and transmitted by Culicoides biting midges, is considered to be one of the most important diseases of domestic ruminants. The first outbreak of bluetongue in Serbia was reported in 2001, when BTV serotype 9 was identified in sampled materials. In 2014, outbreak of BTV-4 in Serbia caused considerable economic losses affecting sheep, cattle and goats. During this outbreak, BTV-4 was recorded in 644 outbreaks within 49 municipalities, part of 17 administrative regions. From the total number of sheep kept in areas affected by bluetongue (n=1 748 110), 2 083 cases (0.2%) were proven to be BTV-4 infected. Total of 206 infected cattle and 24 infected goats were reported during this investigation period, which represents 0.06% and 0.03% of the total number of cattle and goats kept in affected areas, respectively. The highest incidence of infected sheep, cattle and goats was recorded on the territory covered by veterinary institute of Nis. Recorded lethality in cattle, sheep and goats was 18.45% (n=38), 48.10% (n=1002) and 54.17% (n=13), respectively. The peak of the outbreak was in September and October when 94.43% of the confirmed positive cases, regardless of the species, was recorded. Monitoring of bluetongue disease in Serbia relies on active surveillance programmes aimed at: (i) identification and tracing of susceptible and potentially infected animals and (ii) detection, distribution and prevalence of insect vectors. Vaccination of sheep is planned to be implemented as a control measure against bluetongue in Serbia.

Development of Multiwalled Carbon Nanotube-Based Immunobiosensor for the Detection of Bluetongue Virus

2021 ◽  
Vol 21 (6) ◽  
pp. 3513-3523
Author(s):  
Madhu Sudan Guray ◽  
Prasad Minakshi ◽  
Basanti Brar ◽  
Ruma Rani ◽  
Upendra P. Lambe
Keyword(s):  
Carbon Nanotube ◽  
Bluetongue Virus ◽  
Diagnostic Methods ◽  
Multiwalled Carbon Nanotube ◽  
Virus Propagation ◽  
Multiwalled Carbon ◽  
Biting Midges ◽  
Animal Products ◽  
Self Assembled Layer ◽  
The Cost

Bluetongue (BT) disease is a noncontagious disease of domestic and wild ruminants (mainly sheep, cattle, deer) caused by the bluetongue virus (BTV) which is an orbivirus of the Reoviridae family and transmitted by vector Culicoides biting midges. It is a reportable disease of considerable socioeconomic concern and of major importance for the international trade of animals and animal products. Conventional diagnostic methods, such as virus propagation and isolation, immunoassays and also various molecular methods have been developed for the detection of the BTV. Here, we present a novel, rapid and pen-side test for the detection of BTV using multiwalled carbon nanotube (MWCNTs) based immunosensor. Though it is not reported yet. The MWCNTs were prepared, characterized and functionalized with carboxyl group. Viral antibodies were conjugated successfully with functionalized MWCNTs and coated on screen printed carbon electrode (SPCE). These SPCE were evaluated by using electrochemical sensor with an antigen specific to BTV antibodies, resulted in the self-assembled layer of antigen–antibody on the surface of SPCE. The approach described in the present study is a prototype for the development of simple and economic diagnostic tool which will provide the routine screening of BT disease at the door of farmers, thereby increasing the income of farmers by decreasing the cost of diagnosis.

Management of North American Culicoides Biting Midges: Current Knowledge and Research Needs

2015 ◽  
Vol 15 (6) ◽  
pp. 374-384 ◽  
Author(s):  
Robert S. Pfannenstiel ◽  
Bradley A. Mullens ◽  
Mark G. Ruder ◽  
Ludek Zurek ◽  
Lee W. Cohnstaedt ◽  
...  
Keyword(s):  
North American ◽  
Current Knowledge ◽  
Research Needs ◽  
Biting Midges ◽  
Culicoides Biting Midges

scholarly journals Spatial and temporal variation in the abundance of Culicoides biting midges (Diptera: Ceratopogonidae) in nine European countries

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Ana Carolina Cuéllar ◽  
Lene Jung Kjær ◽  
Carsten Kirkeby ◽  
Henrik Skovgard ◽  
Søren Achim Nielsen ◽  
...  
Keyword(s):  
Temporal Variation ◽  
European Countries ◽  
Spatial And Temporal Variation ◽  
Biting Midges ◽  
Culicoides Biting Midges
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