scholarly journals Southern rice black‐streaked dwarf virus hijacks SNARE complex of its insect vector for its effective transmission to rice

2021 ◽  
Author(s):  
Lu Zhang ◽  
Wenwen Liu ◽  
Xiaowan Zhang ◽  
Li Li ◽  
Xifeng Wang
Keyword(s):  
Snare Complex ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Effective Transmission

scholarly journals A novel method for transmitting southern rice black-streaked dwarf virus to rice without insect vector

2017 ◽  
Vol 14 (1) ◽  
Author(s):  
Lu Yu ◽  
Jing Shi ◽  
Lianlian Cao ◽  
Guoping Zhang ◽  
Wenli Wang ◽  
...  
Keyword(s):  
Dwarf Virus ◽  
Insect Vector ◽  
Novel Method

ADP ribosylation factor 1 facilitates spread of wheat dwarf virus in its insect vector

2019 ◽  
Vol 21 (9) ◽  
Author(s):  
Hui Wang ◽  
Yan Liu ◽  
Lu Zhang ◽  
Jiban Kumar Kundu ◽  
Wenwen Liu ◽  
...  
Keyword(s):  
Dwarf Virus ◽  
Insect Vector ◽  
Wheat Dwarf Virus ◽  
Adp Ribosylation ◽  
Adp Ribosylation Factor

scholarly journals Rice black-streaked dwarf virus P10 suppresses protein kinase C in insect vector through changing the subcellular localization of LsRACK1

2019 ◽  
Vol 374 (1767) ◽  
pp. 20180315 ◽  
Author(s):  
Lina Lu ◽  
Qi Wang ◽  
Deqing Huang ◽  
Qiufang Xu ◽  
Xueping Zhou ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Subcellular Localization ◽  
Fluorescent Protein ◽  
Brown Planthopper ◽  
Subcellular Fractionation ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Kinase C ◽  
Green Fluorescent

Rice black-streaked dwarf virus (RBSDV) was known to be transmitted by the small brown planthopper (SBPH) in a persistent, circulative and propagative manner in nature. Here, we show that RBSDV major outer capsid protein (also known as P10) suppresses the protein kinase C (PKC) activity of SBPH through interacting with the receptor for activated protein kinase C 1 (LsRACK1). The N terminal of P10 (amino acids (aa) 1–270) and C terminal of LsRACK1 (aa 268–315) were mapped as crucial for the interaction. Confocal microscopy and subcellular fractionation showed that RBSDV P10 fused to enhanced green fluorescent protein formed vesicular structures associated with endoplasmic reticulum (ER) membranes in Spodoptera frugiperda nine cells. Our results also indicated that RBSDV P10 retargeted the initial subcellular localization of LsRACK1 from cytoplasm and cell membrane to ER and affected the function of LsRACKs to activate PKC. Inhibition of RACK1 by double stranded RNA-induced gene silencing significantly promoted the replication of RBSDV in SBPH. In addition, the PKC pathway participates in the antivirus innate immune response of SBPH. This study highlights that RACK1 negatively regulates the accumulation of RBSDV in SBPH through activating the PKC signalling pathway, and RBSDV P10 changes the subcellular localization of LsRACK1 and affects its function to activate PKC. This article is part of the theme issue ‘Biotic signalling sheds light on smart pest management’.

Nonstructural protein Pns12 of rice dwarf virus is a principal regulator for viral replication and infection in its insect vector

2015 ◽  
Vol 210 ◽  
pp. 54-61 ◽  
Author(s):  
Qian Chen ◽  
Hongyan Chen ◽  
Dongsheng Jia ◽  
Qianzhuo Mao ◽  
Lianhui Xei ◽  
...  
Keyword(s):  
Viral Replication ◽  
Nonstructural Protein ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Rice Dwarf Virus

scholarly journals Southern Rice Black-Streaked Dwarf Virus Infection Improves Host Suitability for Its Insect Vector, Sogatella furcifera (Hemiptera: Delphacidae)

2014 ◽  
Vol 107 (1) ◽  
pp. 92-97 ◽  
Author(s):  
Jie Zhang ◽  
Xue Zheng ◽  
Yongdui Chen ◽  
Jian Hu ◽  
Jiahong Dong ◽  
...  
Keyword(s):  
Virus Infection ◽  
Host Suitability ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Sogatella Furcifera

scholarly journals Adverse Effects of Rice gall dwarf virus upon its Insect Vector Recilia dorsalis (Hemiptera: Cicadellidae)

Plant Disease ◽  
2016 ◽  
Vol 100 (4) ◽  
pp. 784-790 ◽  
Author(s):  
Yong Chen ◽  
Chengcong Lu ◽  
Manman Li ◽  
Wei Wu ◽  
Guohui Zhou ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Molecular Level ◽  
Southern China ◽  
Culture Method ◽  
Protein Product ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Direct Effects ◽  
Rice Gall Dwarf Virus ◽  
Polymerase Chain

Rice gall dwarf virus (RGDV), a plant reovirus that threatens rice production in Southeast Asia and Southern China, is transmitted by the leafhopper vector Recilia dorsalis in a persistent-propagative manner. To assess the direct effects of RGDV on R. dorsalis, we established an infected leafhopper population from eggs laid by viruliferous females using the water-soaked filter paper culture method. Life history parameters indicated that the virus was harmful to its vector in terms of all biotic indices, including reduced survival rate, emergence rate, fecundity, and longevity of adults, compared with a nonviruliferous control population. Those findings were supported by systematic monitoring of viruliferous rates of R. dorsalis in different overwintering generations. To better elucidate the adverse effects of RGDV on its vector, we measured fecundity at the molecular level using quantitative reverse-transcription polymerase chain reaction and Western blot assays, which revealed differential expression of vitellogenin (Vg) in viruliferous versus nonviruliferous adult females. We infer that RGDV reduced levels of Vg transcript and protein product, resulting in the lower fecundity of its vector. Overall, this study demonstrates how RGDV exerts an adverse effect on R. dorsalis, which hinders the expansion of viruliferous populations of the insect.

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