scholarly journals The RpoN2‐PilRX regulatory system governs type IV pilus gene transcription and is required for bacterial motility and virulence in Xanthomonas oryzae pv. oryzae

2020 ◽  
Vol 21 (5) ◽  
pp. 652-666
Author(s):  
Chao Yu ◽  
Doan-Phuong Nguyen ◽  
Zhaoyu Ren ◽  
Jianan Liu ◽  
Fenghuan Yang ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Regulatory System ◽  
Xanthomonas Oryzae ◽  
Bacterial Motility ◽  
Type Iv Pilus ◽  
Type Iv

The Xanthomonas oryzae pv. oryzae type IV pilus alignment subcomplex protein PilN contributes to regulation of bacterial surface‐associated behaviours and T3SS system

2020 ◽  
Vol 69 (4) ◽  
pp. 744-755
Author(s):  
Yilang Li ◽  
Yichao Yan ◽  
Songge Deng ◽  
Cuiping Zhang ◽  
Fazal Haq ◽  
...  
Keyword(s):  
Xanthomonas Oryzae ◽  
Type Iv Pilus ◽  
Bacterial Surface ◽  
Type Iv

scholarly journals Streptococcus sanguinisNoncodingcia-Dependent Small RNAs Negatively Regulate Expression of Type IV Pilus Retraction ATPase PilT and Biofilm Formation

2017 ◽  
Vol 86 (3) ◽  
Author(s):  
Chiaki Ota ◽  
Hirobumi Morisaki ◽  
Masanobu Nakata ◽  
Takafumi Arimoto ◽  
Haruka Fukamachi ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Small Rnas ◽  
Regulatory System ◽  
Luciferase Reporter ◽  
Nucleotide Exchange ◽  
Type Iv Pilus ◽  
Mobility Shift ◽  
Putative Gene ◽  
Content Type ◽  
Type Iv

ABSTRACTSmall noncoding RNAs (sRNAs) have been identified as important regulators of gene expression in various cellular processes.cia-dependent small RNAs (csRNAs), a group of sRNAs that are controlled by the two-component regulatory system CiaRH, are widely conserved in streptococci, but their targets have been identified only inStreptococcus pneumoniae.Streptococcus sanguinis, a pioneer colonizer of teeth and one of the most predominant bacteria in the early oral biofilm, has been shown to have six csRNAs. Using computational target prediction and the luciferase reporter assay, we identifiedpilT, a constituent of the type IV pilus operon, as a negative regulatory target for one of the csRNAs, namely, csRNA1-1, inS. sanguinis. RNA-RNA electrophoretic mobility shift assay using a nucleotide exchange mutant of csRNA1-1 revealed that csRNA1-1 binds directly topilTmRNA. In addition, csRNA1-1 and csRNA1-2, a putative gene duplication product of csRNA1-1 that is tandemly located in theS. sanguinisgenome, negatively regulatedS. sanguinisbiofilm formation. These results suggest the involvement of csRNAs in the colonization step ofS. sanguinis.

scholarly journals Evolutionary rewiring: a modified prokaryotic gene-regulatory pathway in chloroplasts

2013 ◽  
Vol 368 (1622) ◽  
pp. 20120260 ◽  
Author(s):  
Sujith Puthiyaveetil ◽  
Iskander M. Ibrahim ◽  
John F. Allen
Keyword(s):  
Green Algae ◽  
Gene Transcription ◽  
Response Regulator ◽  
Regulatory System ◽  
Photosynthetic Electron Transport ◽  
Sensor Kinase ◽  
Ps I ◽  
Component Systems ◽  
Two Component Systems ◽  
Gene Regulatory

Photosynthetic electron transport regulates chloroplast gene transcription through the action of a bacterial-type sensor kinase known as chloroplast sensor kinase (CSK). CSK represses photosystem I (PS I) gene transcription in PS I light and thus initiates photosystem stoichiometry adjustment. In cyanobacteria and in non-green algae, CSK homologues co-exist with their response regulator partners in canonical bacterial two-component systems. In green algae and plants, however, no response regulator partner of CSK is found. Yeast two-hybrid analysis has revealed interaction of CSK with sigma factor 1 (SIG1) of chloroplast RNA polymerase. Here we present further evidence for the interaction between CSK and SIG1. We also show that CSK interacts with quinone. Arabidopsis SIG1 becomes phosphorylated in PS I light, which then specifically represses transcription of PS I genes. In view of the identical signalling properties of CSK and SIG1 and of their interactions, we suggest that CSK is a SIG1 kinase. We propose that the selective repression of PS I genes arises from the operation of a gene-regulatory phosphoswitch in SIG1. The CSK-SIG1 system represents a novel, rewired chloroplast-signalling pathway created by evolutionary tinkering. This regulatory system supports a proposal for the selection pressure behind the evolutionary stasis of chloroplast genes.

scholarly journals Type IV Pilus Assembly Proficiency and Dynamics Influence Pilin Subunit Phospho-Form Macro- and Microheterogeneity in Neisseria gonorrhoeae

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e96419 ◽  
Author(s):  
Åshild Vik ◽  
Jan Haug Anonsen ◽  
Finn Erik Aas ◽  
Finn Terje Hegge ◽  
Norbert Roos ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Type Iv Pilus ◽  
Type Iv ◽  
Pilin Subunit ◽  
Pilus Assembly

Identification and initial characterization of a new pair of sibling sRNAs of Neisseria gonorrhoeae involved in type IV pilus biogenesis

Microbiology ◽  
2021 ◽  
Vol 167 (9) ◽  
Author(s):  
Marie Zachary ◽  
Susanne Bauer ◽  
Maximilian Klepsch ◽  
Katharina Wagler ◽  
Bruno Hüttel ◽  
...  
Keyword(s):  
Target Genes ◽  
Target Prediction ◽  
Type Iv Pilus ◽  
Content Type ◽  
Type Iv ◽  
Gene Expression Control ◽  
Initial Characterization ◽  
Pilus Biogenesis ◽  
Regulatory Rnas

Non-coding regulatory RNAs mediate post-transcriptional gene expression control by a variety of mechanisms relying mostly on base-pairing interactions with a target mRNA. Though a plethora of putative non-coding regulatory RNAs have been identified by global transcriptome analysis, knowledge about riboregulation in the pathogenic Neisseriae is still limited. Here we report the initial characterization of a pair of sRNAs of N. gonorrhoeae , TfpR1 and TfpR2, which exhibit a similar secondary structure and identical single-stranded seed regions, and therefore might be considered as sibling sRNAs. By combination of in silico target prediction and sRNA pulse expression followed by differential RNA sequencing we identified target genes of TfpR1 which are involved in type IV pilus biogenesis and DNA damage repair. We provide evidence that members of the TfpR1 regulon can also be targeted by the sibling TfpR2.

scholarly journals Purification and Three-Dimensional Electron Microscopy Structure of the Neisseria meningitidis Type IV Pilus Biogenesis Protein PilG

2007 ◽  
Vol 189 (17) ◽  
pp. 6389-6396 ◽  
Author(s):  
Richard F. Collins ◽  
Muhammad Saleem ◽  
Jeremy P. Derrick
Keyword(s):  
Electron Microscopy ◽  
Neisseria Meningitidis ◽  
Metal Ion ◽  
Polyacrylamide Gel Electrophoresis ◽  
Three Dimensional ◽  
Type Ii Secretion ◽  
Type Ii ◽  
Type Iv Pilus ◽  
Type Iv ◽  
Pilus Biogenesis

ABSTRACT Type IV pili are surface-exposed retractable fibers which play a key role in the pathogenesis of Neisseria meningitidis and other gram-negative pathogens. PilG is an integral inner membrane protein and a component of the type IV pilus biogenesis system. It is related by sequence to the extensive GspF family of secretory proteins, which are involved in type II secretion processes. PilG was overexpressed and purified from Escherichia coli membranes by detergent extraction and metal ion affinity chromatography. Analysis of the purified protein by perfluoro-octanoic acid polyacrylamide gel electrophoresis showed that PilG formed dimers and tetramers. A three-dimensional (3-D) electron microscopy structure of the PilG multimer was determined using single-particle averaging applied to samples visualized by negative staining. Symmetry analysis of the unsymmetrized 3-D volume provided further evidence that the PilG multimer is a tetramer. The reconstruction also revealed an asymmetric bilobed structure approximately 125 Å in length and 80 Å in width. The larger lobe within the structure was identified as the N terminus by location of Ni-nitrilotriacetic acid nanogold particles to the N-terminal polyhistidine tag. We propose that the smaller lobe corresponds to the periplasmic domain of the protein, with the narrower “waist” region being the transmembrane section. This constitutes the first report of a 3-D structure of a member of the GspF family and suggests a physical basis for the role of the protein in linking cytoplasmic and periplasmic protein components of the type II secretion and type IV pilus biogenesis systems.

scholarly journals Genomic and Gene-Expression Comparisons among Phage-Resistant Type-IV Pilus Mutants of Pseudomonas syringae pathovar phaseolicola

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0144514 ◽  
Author(s):  
Mark Sistrom ◽  
Derek Park ◽  
Heath E. O’Brien ◽  
Zheng Wang ◽  
David S. Guttman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pseudomonas Syringae ◽  
Type Iv Pilus ◽  
Type Iv

scholarly journals The role of core and accessory type IV pilus genes in natural transformation and twitching motility in the bacterium Acinetobacter baylyi

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0182139 ◽  
Author(s):  
Colleen G. Leong ◽  
Rebecca A. Bloomfield ◽  
Caroline A. Boyd ◽  
Amber J. Dornbusch ◽  
Leah Lieber ◽  
...  
Keyword(s):  
Natural Transformation ◽  
Twitching Motility ◽  
Type Iv Pilus ◽  
Acinetobacter Baylyi ◽  
Type Iv
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