scholarly journals A barley SKP1-like protein controls abundance of the susceptibility factor RACB and influences the interaction of barley with the barley powdery mildew fungus

2015 ◽  
Vol 17 (2) ◽  
pp. 184-195 ◽  
Author(s):  
Tina Reiner ◽  
Caroline Hoefle ◽  
Ralph Hückelhoven
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Fungus ◽  
Susceptibility Factor ◽  
Barley Powdery Mildew

scholarly journals ROP INTERACTIVE PARTNER b interacts with RACB and supports fungal penetration into barley epidermal cells

2019 ◽  
Author(s):  
Christopher McCollum ◽  
Stefan Engelhardt ◽  
Lukas Weiss ◽  
Ralph Hückelhoven
Keyword(s):  
Plasma Membrane ◽  
Powdery Mildew ◽  
Coiled Coil ◽  
Cell Polarization ◽  
Epidermal Cells ◽  
In Planta ◽  
Powdery Mildew Fungus ◽  
Susceptibility Factor ◽  
Polar Cell ◽  
Barley Powdery Mildew

AbstractRHO of Plants (ROP) G-proteins are key components of cell polarization processes in plant development. The barley (Hordeum vulgare) ROP protein RACB, is a susceptibility factor in the interaction of barley with the barley powdery mildew fungus Blumeria graminis f.sp. hordei (Bgh). RACB also drives polar cell development, and this function might be coopted during formation of fungal haustoria in barley epidermal cells. In order to understand RACB signaling during the interaction of barley with Bgh, we searched for potential downstream interactors of RACB. Here, we show that ROP INTERACTIVE PARTNER b (RIPb, synonym: INTERACTOR OF CONSTITUTIVE ACTIVE ROP b; ICRb) directly interacts with RACB in yeast and in planta. Over-expression of RIPb supports susceptibility of barley to Bgh. RIPb further interacts with itself at microtubules. However, the interaction with activated RACB takes place at the plasma membrane. Both, RIPb and RACB are recruited to the site of fungal attack around the neck of developing haustoria suggesting locally enhanced ROP activity. We further assigned different functions to different domains of the RIPb protein. The N-terminal coiled-coil CC1 domain is required for microtubule localization, while the C-terminal coiled-coil CC2 domain is sufficient to interact with RACB and to fulfill a function in susceptibility at the plasma membrane. Hence, RIPb appears to be localized at microtubules and is then recruited by activated RACB for a function at the plasma membrane during formation of the haustorial complex.One Sentence summaryRIPb acts downstream of the powdery mildew susceptibility factor RACB of barley and influences susceptibility

Impact of proquinazid on appressorial development of the barley powdery mildew fungus Blumeria graminis f.sp hordei

2009 ◽  
Vol 94 (2-3) ◽  
pp. 127-132 ◽  
Author(s):  
Sally R. Gilbert ◽  
Hans J. Cools ◽  
Bart A. Fraaije ◽  
Andy M. Bailey ◽  
John A. Lucas
Keyword(s):  
Powdery Mildew ◽  
Blumeria Graminis ◽  
Powdery Mildew Fungus ◽  
Barley Powdery Mildew

scholarly journals The isoelectric point of proteins influences their translocation to the extrahaustorial matrix of the barley powdery mildew fungus

2019 ◽  
Vol 21 (12) ◽  
Author(s):  
Lara Smigielski ◽  
Geziel B. Aguilar ◽  
Mark Kwaaitaal ◽  
Wen‐Jing Zhang ◽  
Hans Thordal‐Christensen
Keyword(s):  
Powdery Mildew ◽  
Isoelectric Point ◽  
Powdery Mildew Fungus ◽  
Barley Powdery Mildew ◽  
Extrahaustorial Matrix

Antifungal Activity of Zanthoxylium schinifolium Against Fusarium graminearum, a Barley Powdery Mildew Fungus.

2008 ◽  
Vol 18 (7) ◽  
pp. 974-979 ◽  
Author(s):  
Byum-Soo Kim ◽  
Han-Su Jang ◽  
Chung-Sig Choi ◽  
Jong-Sik Kim ◽  
Gi-Seok Kwon ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Powdery Mildew ◽  
Fusarium Graminearum ◽  
Powdery Mildew Fungus ◽  
Barley Powdery Mildew

scholarly journals Host-Induced Gene Silencing in Barley Powdery Mildew Reveals a Class of Ribonuclease-Like Effectors

2013 ◽  
Vol 26 (6) ◽  
pp. 633-642 ◽  
Author(s):  
Clara Pliego ◽  
Daniela Nowara ◽  
Giulia Bonciani ◽  
Dana M. Gheorghe ◽  
Ruo Xu ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Gene Silencing ◽  
Transcript Abundance ◽  
Powdery Mildew Fungus ◽  
Host Cell Death ◽  
Barley Powdery Mildew ◽  
Biotrophic Pathogens ◽  
Powdery Mildew Fungi ◽  
Bona Fide ◽  
Do So

Obligate biotrophic pathogens of plants must circumvent or counteract defenses to guarantee accommodation inside the host. To do so, they secrete a variety of effectors that regulate host immunity and facilitate the establishment of pathogen feeding structures called haustoria. The barley powdery mildew fungus Blumeria graminis f. sp. hordei produces a large number of proteins predicted to be secreted from haustoria. Fifty of these Blumeria effector candidates (BEC) were screened by host-induced gene silencing (HIGS), and eight were identified that contribute to infection. One shows similarity to β-1,3 glucosyltransferases, one to metallo-proteases, and two to microbial secreted ribonucleases; the remainder have no similarity to proteins of known function. Transcript abundance of all eight BEC increases dramatically in the early stages of infection and establishment of haustoria, consistent with a role in that process. Complementation analysis using silencing-insensitive synthetic cDNAs demonstrated that the ribonuclease-like BEC 1011 and 1054 are bona fide effectors that function within the plant cell. BEC1011 specifically interferes with pathogen-induced host cell death. Both are part of a gene superfamily unique to the powdery mildew fungi. Structural modeling was consistent, with BEC1054 adopting a ribonuclease-like fold, a scaffold not previously associated with effector function.

Reconstitution of cyanogenesis in barley (Hordeum vulgare L.) and its implications for resistance against the barley powdery mildew fungus

Planta ◽  
2005 ◽  
Vol 223 (5) ◽  
pp. 1010-1023 ◽  
Author(s):  
Kirsten A. Nielsen ◽  
Maria Hrmova ◽  
Janni Nyvang Nielsen ◽  
Karin Forslund ◽  
Stefan Ebert ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Powdery Mildew ◽  
Hordeum Vulgare L ◽  
Powdery Mildew Fungus ◽  
Barley Powdery Mildew

scholarly journals Barley ROP Binding Kinase1 Is Involved in Microtubule Organization and in Basal Penetration Resistance to the Barley Powdery Mildew Fungus

2012 ◽  
Vol 159 (1) ◽  
pp. 311-320 ◽  
Author(s):  
Christina Huesmann ◽  
Tina Reiner ◽  
Caroline Hoefle ◽  
Jutta Preuss ◽  
Manuela E. Jurca ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Penetration Resistance ◽  
Powdery Mildew Fungus ◽  
Microtubule Organization ◽  
Barley Powdery Mildew
Sign in / Sign up

Export Citation Format

Share Document