scholarly journals The AreA transcription factor mediates the regulation of deoxynivalenol (DON) synthesis by ammonium and cyclic adenosine monophosphate (cAMP) signalling inFusarium graminearum

2015 ◽  
Vol 16 (9) ◽  
pp. 987-999 ◽  
Author(s):  
Rui Hou ◽  
Cong Jiang ◽  
Qian Zheng ◽  
Chenfang Wang ◽  
Jin-Rong Xu
Keyword(s):  
Transcription Factor ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Camp Signalling

scholarly journals Imaging cAMP nanodomains in the heart

2019 ◽  
Vol 47 (5) ◽  
pp. 1383-1392 ◽  
Author(s):  
Ying-Chi Chao ◽  
Nicoletta C. Surdo ◽  
Sergio Pantano ◽  
Manuela Zaccolo
Keyword(s):  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Cardiac Contraction ◽  
Cellular Functions ◽  
Fluorescent Reporters ◽  
Sympathetic Control ◽  
Camp Signalling ◽  
Signalling Network ◽  
Contraction And Relaxation ◽  
Differential Phosphorylation

Abstract 3′-5′-cyclic adenosine monophosphate (cAMP) is a ubiquitous second messenger that modulates multiple cellular functions. It is now well established that cAMP can mediate a plethora of functional effects via a complex system of local regulatory mechanisms that result in compartmentalized signalling. The use of fluorescent probes to monitor cAMP in intact, living cells have been instrumental in furthering our appreciation of this ancestral and ubiquitous pathway and unexpected details of the nano-architecture of the cAMP signalling network are starting to emerge. Recent evidence shows that sympathetic control of cardiac contraction and relaxation is achieved via generation of multiple, distinct pools of cAMP that lead to differential phosphorylation of target proteins localized only tens of nanometres apart. The specific local control at these nanodomains is enabled by a distinct signalosome where effectors, targets, and regulators of the cAMP signal are clustered. In this review, we focus on recent advances using targeted fluorescent reporters for cAMP and how they have contributed to our current understanding of nanodomain cAMP signalling in the heart. We briefly discuss how this information can be exploited to design novel therapies and we highlight some of the questions that remain unanswered.

scholarly journals Enhancement of Monascus yellow pigments production by activating the cAMP signalling pathway in Monascus purpureus HJ11

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Jiawei Liu ◽  
Yun Du ◽  
Hongmin Ma ◽  
Xiaolin Pei ◽  
Mu Li
Keyword(s):  
Gene Knockout ◽  
Enzymatic Reaction ◽  
Cyclic Adenosine Monophosphate ◽  
Fold Increase ◽  
Adenosine Monophosphate ◽  
Signalling Pathway ◽  
Knockout Mutant ◽  
Camp Phosphodiesterase ◽  
Camp Signalling

Abstract Background Monascus azaphilone pigments (MonAzPs), which were produced by Monascus species, have been used as important food colorant and food supplements for more than one billion people during their daily life. Moreover, MonAzPs recently have received more attention because of their diverse physiological activities. However, the high microbial production of MonAzPs is still not always guaranteed. Herein, the aim of this study was to develop an efficient biotechnological process for MonAzPs production. Results In this study, exogenous cyclic adenosine monophosphate (cAMP) treatment not only induced MonAzPs production, but also stimulated the expression of a cAMP phosphodiesterase gene, named as mrPDE, in M. purpureus HJ11. Subsequently, MrPDE was identified as a cAMP phosphodiesterase by in vitro enzymatic reaction with purified enzyme. Further, a gene knockout mutant of mrPDE was constructed to verify the activation of cAMP signalling pathway. Deletion of mrPDE in M. purpureus HJ11 improved cAMP concentration by 378% and enhanced PKA kinase activity 1.5-fold, indicating that activation of cAMP signalling pathway was achieved. The ΔmrPDE strain produced MonAzPs at 8563 U/g, with a 2.3-fold increase compared with the WT strain. Moreover, the NAPDH/NADP+ ratio of the ΔmrPDE strain was obviously higher than that of the wild type strain, which led to a higher proportion of yellow MonAzPs. With fed-batch fermentation of the ΔmrPDE strain, the production and yield of MonAzPs achieved 332.1 U/mL and 8739 U/g. Conclusions A engineered M. purpureus strain for high MonAzPs production was successfully developed by activating the cAMP signalling pathway. This study not only describes a novel strategy for development of MonAzPs-producing strain, but also provides a roadmap for engineering efforts towards the production of secondary metabolism in other filamentous fungi.

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