scholarly journals PsMPK7, a stress-associated mitogen-activated protein kinase (MAPK) inPhytophthora sojae, is required for stress tolerance, reactive oxygenated species detoxification, cyst germination, sexual reproduction and infection of soybean

2014 ◽  
Vol 16 (1) ◽  
pp. 61-70 ◽  
Author(s):  
Jian Gao ◽  
Mingna Cao ◽  
Wenwu Ye ◽  
Haiyang Li ◽  
Liang Kong ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Sexual Reproduction ◽  
Stress Tolerance ◽  
Mitogen Activated Protein Kinase ◽  
Mitogen Activated Protein ◽  
Cyst Germination

scholarly journals Overexpression of a Mitogen-Activated Protein Kinase SlMAPK3 Positively Regulates Tomato Tolerance to Cadmium and Drought Stress

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 556 ◽  
Author(s):  
Tayeb Muhammad ◽  
Jie Zhang ◽  
Yalin Ma ◽  
Yushun Li ◽  
Fei Zhang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Protein Kinase ◽  
Transgenic Plants ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Germination Rate ◽  
Mitogen Activated Protein Kinase ◽  
Enzymatic Antioxidants ◽  
Abiotic Stressors ◽  
Mitogen Activated Protein

Mitogen-activated protein kinases (MAPKs) activation is a common defense response of plants to a range of abiotic stressors. SlMPK3, a serine-threonine protein kinase, has been reported as an important member of protein kinase cascade that also functions on plant stress tolerance. In this study, we cloned SlMPK3 from tomato and studied its role in cadmium (Cd2+) and drought tolerance. The results showed that transcripts of SlMAPK3 differentially accumulated in various plant tissues and were remarkably induced by different abiotic stressors and exogenous hormone treatments. Overexpression of SlMAPK3 increased tolerance to Cd2+ and drought as reflected by an increased germination rate and improved seedling growth. Furthermore, transgenic plants overexpressing SlMAPK3 showed an increased leaf chlorophyll content, root biomass accumulation and root activity under Cd2+ stress. Chlorophyll fluorescence analysis revealed that transgenic plants demonstrated an increased photosynthetic activity as well as contents of chlorophyll, proline, and sugar under drought stress. Notably, cadmium- and drought-induced oxidative stress was substantially attenuated in SlMAPK3 overexpressing plants as evidenced by lower malondialdehyde and hydrogen peroxide accumulation, and increased activity and transcript abundance of enzymatic antioxidants under stress conditions compared to that of wild-type. Our findings provide solid evidence that overexpression of SlMAPK3 gene in tomato positively regulates tolerance to Cd2+ and drought stress, which may have strengthen the molecular understanding of SlMAPK3 gene to improve abiotic stress tolerance.

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