scholarly journals Bis ‐molybdopterin guanine dinucleotide modulates hemolysin expression under anaerobiosis and contributes to fitness in vivo in uropathogenic Escherichia coli

2021 ◽  
Author(s):  
Xinyang Zhang ◽  
Dongyan Huang ◽  
Zihui Zhao ◽  
Xuwang Cai ◽  
Wentong Cai ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli ◽  
Molybdopterin Guanine Dinucleotide

scholarly journals An infection-induced RhoB-Beclin 1-Hsp90 complex enhances clearance of uropathogenic Escherichia coli

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Chunhui Miao ◽  
Mingyu Yu ◽  
Geng Pei ◽  
Zhenyi Ma ◽  
Lisong Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Treatment Strategies ◽  
Uropathogenic Escherichia Coli ◽  
Beclin 1 ◽  
Host Cells ◽  
Infection Model ◽  
Intracellular Bacteria ◽  
Bladder Epithelium ◽  
Binding Residue

AbstractHost cells use several anti-bacterial pathways to defend against pathogens. Here, using a uropathogenic Escherichia coli (UPEC) infection model, we demonstrate that bacterial infection upregulates RhoB, which subsequently promotes intracellular bacteria clearance by inducing LC3 lipidation and autophagosome formation. RhoB binds with Beclin 1 through its residues at 118 to 140 and the Beclin 1 CCD domain, with RhoB Arg133 being the key binding residue. Binding of RhoB to Beclin 1 enhances the Hsp90-Beclin 1 interaction, preventing Beclin 1 degradation. RhoB also directly interacts with Hsp90, maintaining RhoB levels. UPEC infections increase RhoB, Beclin 1 and LC3 levels in bladder epithelium in vivo, whereas Beclin 1 and LC3 levels as well as UPEC clearance are substantially reduced in RhoB+/− and RhoB−/− mice upon infection. We conclude that when stimulated by UPEC infections, host cells promote UPEC clearance through the RhoB-Beclin 1-HSP90 complex, indicating RhoB may be a useful target when developing UPEC treatment strategies.

In Vitro and in Vivo Adherence of Uropathogenic Escherichia Coli Strains

1986 ◽  
Vol 135 (6) ◽  
pp. 1319-1321 ◽  
Author(s):  
Walter J. Hopkins ◽  
Jean L. Jensen ◽  
David T. Uehling ◽  
Edward Balish
Keyword(s):  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli

scholarly journals Novel Three-Dimensional Organoid Model for Evaluation of the Interaction of Uropathogenic Escherichia coli with Terminally Differentiated Human Urothelial Cells

2006 ◽  
Vol 74 (1) ◽  
pp. 750-757 ◽  
Author(s):  
Yarery C. Smith ◽  
Kerian K. Grande ◽  
Susan B. Rasmussen ◽  
Alison D. O'Brien
Keyword(s):  
Escherichia Coli ◽  
Three Dimensional ◽  
Uropathogenic Escherichia Coli ◽  
Urothelial Cells ◽  
Human Bladder ◽  
Upec Strain ◽  
Tissue Specific ◽  
Microgravity Conditions ◽  
Uroepithelial Cells

ABSTRACT Human bladder 5637 cells cultivated under microgravity conditions formed organoids that displayed characteristics of in vivo tissue-specific differentiation. Uropathogenic Escherichia coli (UPEC) strain CP9 colonized and penetrated the organoids and induced α-hemolysin-mediated exfoliation of uroepithelial cells. We propose these uro-organoids as models that simulate the interactions between UPEC and terminally differentiated human urothelium.

scholarly journals In vitro and in vivo persistence of IncN plasmids carrying qnr genes in uropathogenic Escherichia coli isolates

2020 ◽  
Vol 22 ◽  
pp. 806-810
Author(s):  
Wilson Dias Segura ◽  
Haissa Pereira Ramos ◽  
Renata Ester de Faria Blanc Amorim ◽  
Ághata Cardoso da Silva Ribeiro ◽  
Edimar Cristiano Pereira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli

scholarly journals Genetically diverse uropathogenic Escherichia coli adopt a common transcriptional program in patients with UTIs

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Anna Sintsova ◽  
Arwen E Frick-Cheng ◽  
Sara Smith ◽  
Ali Pirani ◽  
Sargurunathan Subashchandrabose ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli ◽  
Transcriptional Program ◽  
Functional Responses ◽  
Translational Machinery ◽  
Tract Infections ◽  
Expression Program ◽  
Fast Growth Rate

Uropathogenic Escherichia coli (UPEC) is the major causative agent of uncomplicated urinary tract infections (UTIs). A common virulence genotype of UPEC strains responsible for UTIs is yet to be defined, due to the large variation of virulence factors observed in UPEC strains. We hypothesized that studying UPEC functional responses in patients might reveal universal UPEC features that enable pathogenesis. Here we identify a transcriptional program shared by genetically diverse UPEC strains isolated from 14 patients during uncomplicated UTIs. Strikingly, this in vivo gene expression program is marked by upregulation of translational machinery, providing a mechanism for the rapid growth within the host. Our analysis indicates that switching to a more specialized catabolism and scavenging lifestyle in the host allows for the increased translational output. Our study identifies a common transcriptional program underlying UTIs and illuminates the molecular underpinnings that likely facilitate the fast growth rate of UPEC in infected patients.

scholarly journals Atg16L1 deficiency confers protection from uropathogenic Escherichia coli infection in vivo

2012 ◽  
Vol 109 (27) ◽  
pp. 11008-11013 ◽  
Author(s):  
C. Wang ◽  
G. R. Mendonsa ◽  
J. W. Symington ◽  
Q. Zhang ◽  
K. Cadwell ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli ◽  
Escherichia Coli Infection

Intranasal immunization with a recombinant truncated FimH adhesin adjuvanted with CpG oligodeoxynucleotides protects mice against uropathogenic Escherichia coli challenge

2006 ◽  
Vol 52 (11) ◽  
pp. 1093-1102 ◽  
Author(s):  
Thelma Verónica Poggio ◽  
José Leonardo La Torre ◽  
Eduardo Alberto Scodeller
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Immunoglobulin A ◽  
Uropathogenic Escherichia Coli ◽  
Cpg Oligodeoxynucleotides ◽  
Specific Immunoglobulin ◽  
Intranasal Vaccine ◽  
Tract Infections ◽  
Vaginal Wash

In this work, we assessed the efficacy of an experimental intranasal vaccine against urinary-tract infections. The vaccine contained a recombinant truncated FimH (rFimHt) adhesin plus CpG oligodeoxynucleotides. The efficacy of the vaccine was compared with that of an intramuscular vaccine that was formulated with the same immunogen plus Freund's adjuvant. Our results show that serum immunoglobulin G titers of vaccinated animals were similarly enhanced in both cases. However, the intranasal vaccine elicited higher vaginal-wash-specific immunoglobulin A titers against rFimHt than the intramuscular route. Both vaccines reduced the in vivo colonization of the bladder by uropathogenic Escherichia coli more than 100-fold in a murine cystitis model. Our results indicate that a recombinant truncated FimH adhesin plus CpG oligodeoxynucleotides is a suitable immunogenic combination that can contribute to the development of a highly efficacious urinary tract infection vaccine.Key words: FimH adhesin, CpG oligodeoxynucleotides, intranasal vaccine.

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