scholarly journals Multi‐scale ensemble properties of the Escherichia coli RNA degradosome

2021 ◽  
Author(s):  
Tom Dendooven ◽  
Giulia Paris ◽  
Alexander V. Shkumatov ◽  
M.S. Islam ◽  
Alister Burt ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multi Scale ◽  
Rna Degradosome

scholarly journals Global analysis of Escherichia coli RNA degradosome function using DNA microarrays

2004 ◽  
Vol 101 (9) ◽  
pp. 2758-2763 ◽  
Author(s):  
J. A. Bernstein ◽  
P.-H. Lin ◽  
S. N. Cohen ◽  
S. Lin-Chao
Keyword(s):  
Escherichia Coli ◽  
Dna Microarrays ◽  
Global Analysis ◽  
Rna Degradosome

scholarly journals Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome

1998 ◽  
Vol 12 (17) ◽  
pp. 2770-2781 ◽  
Author(s):  
N. F. Vanzo ◽  
Y. S. Li ◽  
B. Py ◽  
E. Blum ◽  
C. F. Higgins ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein Interactions ◽  
Ribonuclease E ◽  
Rna Degradosome

Escherichia coli RNA Degradosome

2001 ◽  
pp. 333-345 ◽  
Author(s):  
Agamemnon J. Carpousis ◽  
Anne Leroy ◽  
Nathalie Vanzo ◽  
Vanessa Khemici
Keyword(s):  
Escherichia Coli ◽  
Rna Degradosome

Analysis of the Escherichia coli RNA degradosome composition by a proteomic approach

Biochimie ◽  
2006 ◽  
Vol 88 (2) ◽  
pp. 151-161 ◽  
Author(s):  
Maria Elena Regonesi ◽  
Marta Del Favero ◽  
Fabrizio Basilico ◽  
Federica Briani ◽  
Louise Benazzi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Proteomic Approach ◽  
Rna Degradosome

A DEAD-box RNA helicase in the Escherichia coli RNA degradosome

Nature ◽  
1996 ◽  
Vol 381 (6578) ◽  
pp. 169-172 ◽  
Author(s):  
Béatrice Py ◽  
Christopher F. Higgins ◽  
Henry M. Krisch ◽  
Agamemnon J. Carpousis
Keyword(s):  
Escherichia Coli ◽  
Rna Helicase ◽  
Dead Box ◽  
Rna Degradosome

scholarly journals The Escherichia coli major exoribonuclease RNase II is a component of the RNA degradosome

2014 ◽  
Vol 34 (6) ◽  
Author(s):  
Feng Lu ◽  
Aziz Taghbalout
Keyword(s):  
Escherichia Coli ◽  
Rna Processing ◽  
Binding Sites ◽  
Rna Degradation ◽  
The Other ◽  
Polynucleotide Phosphorylase ◽  
Cell Extracts ◽  
Domains Of Life ◽  
Rna Degradosome ◽  
Rnase Ii

Multiprotein complexes that carry out RNA degradation and processing functions are found in cells from all domains of life. In Escherichia coli, the RNA degradosome, a four-protein complex, is required for normal RNA degradation and processing. In addition to the degradosome complex, the cell contains other ribonucleases that also play important roles in RNA processing and/or degradation. Whether the other ribonucleases are associated with the degradosome or function independently is not known. In the present work, IP (immunoprecipitation) studies from cell extracts showed that the major hydrolytic exoribonuclease RNase II is associated with the known degradosome components RNaseE (endoribonuclease E), RhlB (RNA helicase B), PNPase (polynucleotide phosphorylase) and Eno (enolase). Further evidence for the RNase II-degradosome association came from the binding of RNase II to purified RNaseE in far western affinity blot experiments. Formation of the RNase II–degradosome complex required the degradosomal proteins RhlB and PNPase as well as a C-terminal domain of RNaseE that contains binding sites for the other degradosomal proteins. This shows that the RNase II is a component of the RNA degradosome complex, a previously unrecognized association that is likely to play a role in coupling and coordinating the multiple elements of the RNA degradation pathways.

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