Functional interaction between ELL transcription elongation factor and Epe1 reveals the role of Epe1 in the regulation of transcription outside heterochromatin

2021 ◽  
Author(s):  
Kumari Sweta ◽  
Nimisha Sharma
Keyword(s):  
Transcription Elongation ◽  
Elongation Factor ◽  
Regulation Of Transcription ◽  
Functional Interaction ◽  
Transcription Elongation Factor

The elongation factors Pandora/Spt6 and Foggy/Spt5 promote transcription in the zebrafish embryo

Development ◽  
2002 ◽  
Vol 129 (7) ◽  
pp. 1623-1632 ◽  
Author(s):  
Brian R. Keegan ◽  
Jessica L. Feldman ◽  
Diana H. Lee ◽  
David S. Koos ◽  
Robert K. Ho ◽  
...  
Keyword(s):  
Transcription Elongation ◽  
Elongation Factor ◽  
Cardiac Differentiation ◽  
Null Alleles ◽  
Pcr Analysis ◽  
Regulation Of Transcription ◽  
Developmental Defects ◽  
Transcription Elongation Factor ◽  
On Line

Precise temporal and spatial control of transcription is a fundamental component of embryonic development. Regulation of transcription elongation can act as a rate-limiting step during mRNA synthesis. The mechanisms of stimulation and repression of transcription elongation during development are not yet understood. We have identified a class of zebrafish mutations (pandora, sk8 and s30) that cause multiple developmental defects, including discrete problems with pigmentation, tail outgrowth, ear formation and cardiac differentiation. We demonstrate that the pandora gene encodes a protein similar to Spt6, a proposed transcription elongation factor. Additionally, the sk8 and s30 mutations are null alleles of the foggy/spt5 locus, which encodes another transcription elongation factor. Through real-time RT-PCR analysis, we demonstrate that Spt6 and Spt5 are both required for efficient kinetics of hsp70 transcription in vivo. Altogether, our results suggest that Spt6 and Spt5 play essential roles of comparable importance for promoting transcription during embryogenesis. This study provides the first genetic evidence for parallel functions of Spt6 and Spt5 in metazoans and establishes a system for the future analysis of transcription elongation during development. Supplemental figure available on-line

scholarly journals Role of human transcription elongation factor DSIF in the suppression of senescence and apoptosis

2009 ◽  
Vol 14 (3) ◽  
pp. 343-354 ◽  
Author(s):  
Toshiharu Komori ◽  
Naoto Inukai ◽  
Tomoko Yamada ◽  
Yuki Yamaguchi ◽  
Hiroshi Handa
Keyword(s):  
Transcription Elongation ◽  
Elongation Factor ◽  
Transcription Elongation Factor

Synthetic Lethal Interactions Suggest a Role for the Saccharomyces cerevisiae Rtf1 Protein in Transcription Elongation

Genetics ◽  
2000 ◽  
Vol 156 (2) ◽  
pp. 535-547 ◽  
Author(s):  
Patrick J Costa ◽  
Karen M Arndt
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rna Polymerase Ii ◽  
Transcription Elongation ◽  
Elongation Factor ◽  
Regulation Of Transcription ◽  
Gene Products ◽  
Synthetic Lethal ◽  
Transcription Elongation Factor ◽  
Synthetic Lethal Interactions ◽  
Ctd Phosphatase

Abstract Strong evidence indicates that transcription elongation by RNA polymerase II (pol II) is a highly regulated process. Here we present genetic results that indicate a role for the Saccharomyces cerevisiae Rtf1 protein in transcription elongation. A screen for synthetic lethal mutations was carried out with an rtf1 deletion mutation to identify factors that interact with Rtf1 or regulate the same process as Rtf1. The screen uncovered mutations in SRB5, CTK1, FCP1, and POB3. These genes encode an Srb/mediator component, a CTD kinase, a CTD phosphatase, and a protein involved in the regulation of transcription by chromatin structure, respectively. All of these gene products have been directly or indirectly implicated in transcription elongation, indicating that Rtf1 may also regulate this process. In support of this view, we show that RTF1 functionally interacts with genes that encode known elongation factors, including SPT4, SPT5, SPT16, and PPR2. We also show that a deletion of RTF1 causes sensitivity to 6-azauracil and mycophenolic acid, phenotypes correlated with a transcription elongation defect. Collectively, our results suggest that Rtf1 may function as a novel transcription elongation factor in yeast.

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