The stationary phase regulator CpcR activates cry gene expression in non‐sporulating cells of Bacillus thuringiensis

2019 ◽  
Vol 113 (4) ◽  
pp. 740-754
Author(s):  
Ruibin Zhang ◽  
Leyla Slamti ◽  
Lei Tong ◽  
Emilie Verplaetse ◽  
Lixia Ma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bacillus Thuringiensis ◽  
Stationary Phase ◽  
Cry Gene

scholarly journals Regulation of cry Gene Expression in Bacillus thuringiensis

Toxins ◽  
2014 ◽  
Vol 6 (7) ◽  
pp. 2194-2209 ◽  
Author(s):  
Chao Deng ◽  
Qi Peng ◽  
Fuping Song ◽  
Didier Lereclus
Keyword(s):  
Gene Expression ◽  
Bacillus Thuringiensis ◽  
Cry Gene

scholarly journals Acinetobacter baylyi Starvation-Induced Genes Identified through Incubation in Long-Term Stationary Phase

2010 ◽  
Vol 76 (14) ◽  
pp. 4905-4908 ◽  
Author(s):  
C. Phoebe Lostroh ◽  
Bruce A. Voyles
Keyword(s):  
Gene Expression ◽  
Stationary Phase ◽  
Batch Culture ◽  
Exponential Growth ◽  
Hypothetical Proteins ◽  
Acinetobacter Baylyi ◽  
Metabolic Gene Expression ◽  
Induced Genes ◽  
Dna Maintenance

ABSTRACT Acinetobacter species encounter cycles of feast and famine in nature. We show that populations of A cinetobacter baylyi strain ADP1 remain dynamic for 6 weeks in batch culture. We created a library of lacZ reporters inserted into SalI sites in the genome and then isolated 30 genes with lacZ insertions whose expression was induced by starvation during long-term stationary phase compared with their expression during exponential growth. The genes encode metabolic, gene expression, DNA maintenance, envelope, and conserved hypothetical proteins.

Genetic analysis of cryIIIA gene expression in Bacillus thuringiensis

Microbiology ◽  
1996 ◽  
Vol 142 (8) ◽  
pp. 2049-2055 ◽  
Author(s):  
S. Salamitou ◽  
H. Agaisse ◽  
A. Bravo ◽  
D. Lereclus
Keyword(s):  
Gene Expression ◽  
Bacillus Thuringiensis ◽  
Genetic Analysis

Induction of yeast histone genes by stimulation of stationary-phase cells

1990 ◽  
Vol 10 (12) ◽  
pp. 6356-6361
Author(s):  
M A Drebot ◽  
L M Veinot-Drebot ◽  
R A Singer ◽  
G C Johnston
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stationary Phase ◽  
Mitotic Cell ◽  
Specific Gene ◽  
Mitotic Cell Cycle ◽  
Histone Genes ◽  
Yeast Saccharomyces Cerevisiae ◽  
Alpha Factor ◽  
Stimulation Of

In the cell cycle of the budding yeast Saccharomyces cerevisiae, expression of the histone genes H2A and H2B of the TRT1 and TRT2 loci is regulated by the performance of "start," the step that also regulates the cell cycle. Here we show that histone production is also subject to an additional form of regulation that is unrelated to the mitotic cell cycle. Expression of histone genes, as assessed by Northern (RNA) analysis, was shown to increase promptly after the stimulation, brought about by fresh medium, that activates stationary-phase cells to reenter the mitotic cell cycle. The use of a yeast mutant that is conditionally blocked in the resumption of proliferation at a step that is not part of the mitotic cell cycle (M.A. Drebot, G.C. Johnston, and R.A. Singer, Proc. Natl. Acad. Sci. 84:7948, 1987) showed that this increased gene expression that occurs upon stimulation of stationary-phase cells took place in the absence of DNA synthesis and without the performance of start. This stimulation-specific gene expression was blocked by the mating pheromone alpha-factor, indicating that alpha-factor directly inhibits expression of these histone genes, independently of start.

scholarly journals Unraveling the regulation of sophorolipid biosynthesis in Starmerella bombicola

2020 ◽  
Vol 20 (3) ◽  
Author(s):  
Sofie Lodens ◽  
Sophie L K W Roelants ◽  
Goedele Luyten ◽  
Robin Geys ◽  
Pieter Coussement ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stationary Phase ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Exponential Phase ◽  
Biosynthetic Gene ◽  
Reporter System ◽  
Gfp Expression ◽  
Qpcr Analysis ◽  
Starmerella Bombicola

ABSTRACT Starmerella bombicola very efficiently produces the secondary metabolites sophorolipids (SLs). Their biosynthesis is not-growth associated and highly upregulated in the stationary phase. Despite high industrial and academic interest, the underlying regulation of SL biosynthesis remains unknown. In this paper, potential regulation of SL biosynthesis through the telomere positioning effect (TPE) was investigated, as the SL gene cluster is located adjacent to a telomere. An additional copy of this gene cluster was introduced elsewhere in the genome to investigate if this results in a decoy of regulation. Indeed, for the new strain, the onset of SL production was shifted to the exponential phase. This result was confirmed by RT-qPCR analysis. The TPE effect was further investigated by developing and applying a suitable reporter system for this non-conventional yeast, enabling non-biased comparison of gene expression between the subtelomeric CYP52M1- and the URA3 locus. This was done with a constitutive endogenous promotor (pGAPD) and one of the endogenous promotors of the SL biosynthetic gene cluster (pCYP52M1). A clear positioning effect was observed for both promotors with significantly higher GFP expression levels at the URA3 locus. No clear GFP upregulation was observed in the stationary phase for any of the new strains.

scholarly journals The environmental stress response causes ribosome loss in aneuploid yeast cells

2020 ◽  
Vol 117 (29) ◽  
pp. 17031-17040 ◽  
Author(s):  
Allegra Terhorst ◽  
Arzu Sandikci ◽  
Abigail Keller ◽  
Charles A. Whittaker ◽  
Maitreya J. Dunham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stress Response ◽  
Stationary Phase ◽  
Environmental Stress ◽  
Budding Yeast ◽  
Expression Patterns ◽  
Yeast Cells ◽  
Specific Gene ◽  
Environmental Stress Response ◽  
Cellular Stresses

Aneuploidy, a condition characterized by whole chromosome gains and losses, is often associated with significant cellular stress and decreased fitness. However, how cells respond to the aneuploid state has remained controversial. In aneuploid budding yeast, two opposing gene-expression patterns have been reported: the “environmental stress response” (ESR) and the “common aneuploidy gene-expression” (CAGE) signature, in which many ESR genes are oppositely regulated. Here, we investigate this controversy. We show that the CAGE signature is not an aneuploidy-specific gene-expression signature but the result of normalizing the gene-expression profile of actively proliferating aneuploid cells to that of euploid cells grown into stationary phase. Because growth into stationary phase is among the strongest inducers of the ESR, the ESR in aneuploid cells was masked when stationary phase euploid cells were used for normalization in transcriptomic studies. When exponentially growing euploid cells are used in gene-expression comparisons with aneuploid cells, the CAGE signature is no longer evident in aneuploid cells. Instead, aneuploid cells exhibit the ESR. We further show that the ESR causes selective ribosome loss in aneuploid cells, providing an explanation for the decreased cellular density of aneuploid cells. We conclude that aneuploid budding yeast cells mount the ESR, rather than the CAGE signature, in response to aneuploidy-induced cellular stresses, resulting in selective ribosome loss. We propose that the ESR serves two purposes in aneuploid cells: protecting cells from aneuploidy-induced cellular stresses and preventing excessive cellular enlargement during slowed cell cycles by down-regulating translation capacity.

Monitoring dynamics of gene expression in yeast during stationary phase

Gene ◽  
1999 ◽  
Vol 236 (1) ◽  
pp. 33-42 ◽  
Author(s):  
Irit Paz ◽  
Jean-Roch Meunier ◽  
Mordechai Choder
Keyword(s):  
Gene Expression ◽  
Stationary Phase

scholarly journals Characterization of a Legionella pneumophila relA Insertion Mutant and Roles of RelA and RpoS in Virulence Gene Expression

2002 ◽  
Vol 184 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Tal Zusman ◽  
Ohad Gal-Mor ◽  
Gil Segal
Keyword(s):  
Gene Expression ◽  
Stationary Phase ◽  
Gene Product ◽  
Legionella Pneumophila ◽  
Type Strain ◽  
Wild Type Strain ◽  
Insertion Mutant ◽  
Wild Type ◽  
Intracellular Growth ◽  
Virulence Gene Expression

ABSTRACT To investigate the involvement of RelA in the regulation of Legionella pneumophila virulence, a deletion substitution was constructed in the relA gene. The relA knockout resulted in an undetectable level of ppGpp in the cells during the stationary phase, but the original level was restored when the relA gene product was supplied on a plasmid. The effect of the relA mutation was examined with two systems that are known to be expressed during the stationary phase in L. pneumophila. Pigment production was found to be dependent on the relA gene product, and only one-half as much pigment was produced by the relA mutant as by the wild-type strain. Flagellum gene expression was also found to be dependent on the relA gene product, as determined with a flaA::lacZ fusion. However, the relA gene product was found to be dispensable for intracellular growth both in HL-60-derived human macrophages and in the protozoan host Acanthamoeba castellanii. To determine the involvement of the relA gene product in expression of L. pneumophila genes required for intracellular growth (icm/dot genes), nine icm::lacZ fusions were constructed, and expression of these fusions in the wild-type strain was compared with their expression in relA mutant strains. Expression of only one of the icm::lacZ fusions was moderately reduced in the relA mutant strain. Expression of the nine icm::lacZ fusions was also examined in a strain containing an insertion in the gene that codes for the stationary-phase sigma factor RpoS, and similar results were obtained. We concluded that RelA is dispensable for intracellular growth of L. pneumophila in the two hosts examined and that both RelA and RpoS play minor roles in L. pneumophila icm/dot gene expression.

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