scholarly journals The fascinating biology behind phage display: filamentous phage assembly

2019 ◽  
Vol 111 (5) ◽  
pp. 1132-1138 ◽  
Author(s):  
Belinda Loh ◽  
Andreas Kuhn ◽  
Sebastian Leptihn
Keyword(s):  
Phage Display ◽  
Filamentous Phage ◽  
Phage Assembly

scholarly journals Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display

2016 ◽  
Vol 7 ◽  
Author(s):  
Dragana Gagic ◽  
Milica Ciric ◽  
Wesley X. Wen ◽  
Filomena Ng ◽  
Jasna Rakonjac
Keyword(s):  
Phage Display ◽  
Microbial Communities ◽  
Filamentous Phage

scholarly journals Generation of Lamprey Monoclonal Antibodies (Lampribodies) Using the Phage Display System

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 868 ◽  
Author(s):  
Khan M. A. Hassan ◽  
John D. Hansen ◽  
Brantley R. Herrin ◽  
Chris T. Amemiya
Keyword(s):  
Monoclonal Antibodies ◽  
Phage Display ◽  
Genomic Rearrangements ◽  
Filamentous Phage ◽  
Human Cell Lines ◽  
Display System ◽  
Phage Display Libraries ◽  
Humoral Antibodies ◽  
Leucine Rich Repeats ◽  
Lymphocyte Receptors

The variable lymphocyte receptors (VLRs) consist of leucine rich repeats (LRRs) and comprise the humoral antibodies produced by lampreys and hagfishes. The diversity of the molecules is generated by stepwise genomic rearrangements of LRR cassettes dispersed throughout the VLRB locus. Previously, target-specific monovalent VLRB antibodies were isolated from sea lamprey larvae after immunization with model antigens. Further, the cloned VLR cDNAs from activated lamprey leukocytes were transfected into human cell lines or yeast to select best binders. Here, we expand on the overall utility of the VLRB technology by introducing it into a filamentous phage display system. We first tested the efficacy of isolating phage into which known VLRB molecules were cloned after a series of dilutions. These experiments showed that targeted VLRB clones could easily be recovered even after extensive dilutions (1 to 109). We further utilized the system to isolate target-specific “lampribodies” from phage display libraries from immunized animals and observed an amplification of binders with relative high affinities by competitive binding. The lampribodies can be individually purified and ostensibly utilized for applications for which conventional monoclonal antibodies are employed.

scholarly journals A filamentous phage display system for N- linked glycoproteins

2010 ◽  
Vol 19 (10) ◽  
pp. 2006-2013 ◽  
Author(s):  
Eda Çelik ◽  
Adam C. Fisher ◽  
Cassandra Guarino ◽  
Thomas J. Mansell ◽  
Matthew P. DeLisa
Keyword(s):  
Phage Display ◽  
Filamentous Phage ◽  
Display System

Filamentous phage assembly: variation on a protein export theme

Gene ◽  
1997 ◽  
Vol 192 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Marjorie Russel ◽  
Nora A Linderoth ◽  
Andrej Šali
Keyword(s):  
Protein Export ◽  
Filamentous Phage ◽  
Phage Assembly

Induction of hepatitis B virus-specific cytotoxic T lymphocytes response in vivo by filamentous phage display vaccine

Vaccine ◽  
2001 ◽  
Vol 19 (20-22) ◽  
pp. 2918-2923 ◽  
Author(s):  
Ying Wan ◽  
Yuzhang Wu ◽  
Jiang Bian ◽  
XiangZhi Wang ◽  
Wei Zhou ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
T Lymphocytes ◽  
Hepatitis B ◽  
Phage Display ◽  
Cytotoxic T Lymphocytes ◽  
Filamentous Phage ◽  
B Virus
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