scholarly journals Global regulatory roles of the c AMP/PKA pathway revealed by phenotypic, transcriptomic and phosphoproteomic analyses in a null mutant of the PKA catalytic subunit in C andida albicans

2017 ◽  
Vol 105 (1) ◽  
pp. 46-64 ◽  
Author(s):  
Chengjun Cao ◽  
Mei Wu ◽  
Jian Bing ◽  
Li Tao ◽  
Xuefen Ding ◽  
...  
Keyword(s):  
Catalytic Subunit ◽  
Null Mutant ◽  
Pka Pathway ◽  
Pka Catalytic Subunit

scholarly journals Protein Kinase A Distribution in Meningioma

Cancers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1686 ◽  
Author(s):  
Caretta ◽  
Denaro ◽  
D’Avella ◽  
Mucignat-Caretta
Keyword(s):  
Brain Tissue ◽  
Catalytic Subunit ◽  
Therapeutic Interventions ◽  
Normal Brain ◽  
Local Concentration ◽  
Correlation Pattern ◽  
Catalytic Subunits ◽  
Tissue Specimens ◽  
Pka Catalytic Subunit

Deregulation of intracellular signal transduction pathways is a hallmark of cancer cells, clearly differentiating them from healthy cells. Differential intracellular distribution of the cAMP-dependent protein kinases (PKA) was previously detected in cell cultures and in vivo in glioblastoma and medulloblastoma. Our goal is to extend this observation to meningioma, to explore possible differences among tumors of different origins and prospective outcomes. The distribution of regulatory and catalytic subunits of PKA has been examined in tissue specimens obtained during surgery from meningioma patients. PKA RI subunit appeared more evenly distributed throughout the cytoplasm, but it was clearly detectable only in some tumors. RII was present in discrete spots, presumably at high local concentration; these aggregates could also be visualized under equilibrium binding conditions with fluorescent 8-substituted cAMP analogues, at variance with normal brain tissue and other brain tumors. The PKA catalytic subunit showed exactly overlapping pattern to RII and in fixed sections could be visualized by fluorescent cAMP analogues. Gene expression analysis showed that the PKA catalytic subunit revealed a significant correlation pattern with genes involved in meningioma. Hence, meningioma patients show a distinctive distribution pattern of PKA regulatory and catalytic subunits, different from glioblastoma, medulloblastoma, and healthy brain tissue. These observations raise the possibility of exploiting the PKA intracellular pathway as a diagnostic tool and possible therapeutic interventions.

scholarly journals Activity, expression and function of a second Drosophila protein kinase A catalytic subunit gene.

Genetics ◽  
1995 ◽  
Vol 141 (4) ◽  
pp. 1507-1520 ◽  
Author(s):  
A Meléndez ◽  
W Li ◽  
D Kalderon
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Essential Gene ◽  
Sequence Similarity ◽  
Catalytic Subunit ◽  
Subunit Gene ◽  
Drosophila Protein ◽  
Pka Catalytic Subunit ◽  
Kinase A

Abstract The DC2 gene was isolated previously on the basis of sequence similarity to DC0, the major Drosophila protein kinase A (PKA) catalytic subunit gene. We show here that the 67-kD Drosophila DC2 protein behaves as a PKA catalytic subunit in vitro. DC2 is transcribed in mesodermal anlagen of early embryos. This expression depends on dorsal but on neither twist nor snail activity. DC2 transcriptional fusions mimic this embryonic expression and are also expressed in subsets of cells in the optic lamina, wing disc and leg discs of third instar larvae. A saturation screen of a small deficiency interval containing DC2 for recessive lethal mutations yielded no DC2 alleles. We therefore isolated new deficiencies to generate deficiency trans-heterozygotes that lacked DC2 activity. These animals were viable and fertile. The absence of DC2 did not affect the viability or phenotype of imaginal disc cells lacking DC0 activity or embryonic hatching of animals with reduced DC0 activity. Furthermore, transgenes expressing DC2 from a DC0 promoter did not efficiently rescue a variety of DC0 mutant phenotypes. These observations indicate that DC2 is not an essential gene and is unlikely to be functionally redundant with DC0, which has multiple unique functions during development.

Stimulation of c-Rel transcriptional activity by PKA catalytic subunit ?

2004 ◽  
Vol 82 (9) ◽  
Author(s):  
Shih-Hung Yu ◽  
Wei-Chung Chiang ◽  
Hsiu-Ming Shih ◽  
Kou-Juey Wu
Keyword(s):  
Transcriptional Activity ◽  
Catalytic Subunit ◽  
Pka Catalytic Subunit ◽  
Stimulation Of

scholarly journals PKA catalytic subunit compartmentation regulates contractile and hypertrophic responses to β-adrenergic signaling

2014 ◽  
Vol 66 ◽  
pp. 83-93 ◽  
Author(s):  
Jason H. Yang ◽  
Renata K. Polanowska-Grabowska ◽  
Jeffrey S. Smith ◽  
Charles W. Shields ◽  
Jeffrey J. Saucerman
Keyword(s):  
Catalytic Subunit ◽  
Adrenergic Signaling ◽  
Pka Catalytic Subunit

Inhibition of CREB Phosphorylation by Conjugates of Adenosine Analogues and Arginine-Rich Peptides, Inhibitors of PKA Catalytic Subunit

ChemBioChem ◽  
2014 ◽  
Vol 16 (2) ◽  
pp. 312-319 ◽  
Author(s):  
Marie Kriisa ◽  
Hedi Sinijärv ◽  
Angela Vaasa ◽  
Erki Enkvist ◽  
Sergiy Kostenko ◽  
...  
Keyword(s):  
Catalytic Subunit ◽  
Adenosine Analogues ◽  
Creb Phosphorylation ◽  
Pka Catalytic Subunit

TSH Control of PKA Catalytic Subunit Activity in Thyroid Cell Cultures

1999 ◽  
Vol 266 (1) ◽  
pp. 15-18 ◽  
Author(s):  
M. Ben Abdelkhalek ◽  
A. Mamoune ◽  
P. Crisanti ◽  
B. Omri ◽  
B. Haye ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Catalytic Subunit ◽  
Thyroid Cell ◽  
Pka Catalytic Subunit

scholarly journals PKA catalytic subunit mutations in adrenocortical Cushing’s adenoma impair association with the regulatory subunit

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Davide Calebiro ◽  
Annette Hannawacker ◽  
Sandra Lyga ◽  
Kerstin Bathon ◽  
Ulrike Zabel ◽  
...  
Keyword(s):  
Catalytic Subunit ◽  
Regulatory Subunit ◽  
Pka Catalytic Subunit
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