scholarly journals Mechanism of type-III protein secretion: Regulation of FlhA conformation by a functionally critical charged-residue cluster

2017 ◽  
Vol 104 (2) ◽  
pp. 234-249 ◽  
Author(s):  
Marc Erhardt ◽  
Paige Wheatley ◽  
Eun A Kim ◽  
Takanori Hirano ◽  
Yang Zhang ◽  
...  
Keyword(s):  
Protein Secretion ◽  
Charged Residue ◽  
Type Iii ◽  
Secretion Regulation ◽  
Type Iii Protein Secretion

Type III protein secretion and inhibition of NF-κB

2003 ◽  
pp. 279-294
Author(s):  
Klaus Ruckdeschel ◽  
Bruno Rouot ◽  
Jürgen Heesemann
Keyword(s):  
Protein Secretion ◽  
Type Iii ◽  
Type Iii Protein Secretion

scholarly journals Comparison of ATPase-Encoding Type III Secretion System hrcN Genes in Biocontrol Fluorescent Pseudomonads and in Phytopathogenic Proteobacteria

2004 ◽  
Vol 70 (9) ◽  
pp. 5119-5131 ◽  
Author(s):  
Fabio Rezzonico ◽  
Geneviève Défago ◽  
Yvan Moënne-Loccoz
Keyword(s):  
Pseudomonas Syringae ◽  
Protein Secretion ◽  
Type Iii Secretion ◽  
Fluorescent Pseudomonads ◽  
Secretion Systems ◽  
Associated Bacteria ◽  
Type Iii ◽  
Encoding Gene ◽  
Type Iii Protein Secretion ◽  
Protein Secretion Systems

ABSTRACT Type III protein secretion systems play a key role in the virulence of many pathogenic proteobacteria, but they also occur in nonpathogenic, plant-associated bacteria. Certain type III protein secretion genes (e.g., hrcC) have been found in Pseudomonas sp. strain SBW25 (and other biocontrol pseudomonads), but other type III protein secretion genes, such as the ATPase-encoding gene hrcN, have not been found. Using both colony hybridization and a PCR approach, we show here that hrcN is nevertheless present in many biocontrol fluorescent pseudomonads. The phylogeny of biocontrol Pseudomonas strains based on partial hrcN sequences was largely congruent with the phylogenies derived from analyses of rrs (encoding 16S rRNA) and, to a lesser extent, biocontrol genes, such as phlD (for 2,4-diacetylphloroglucinol production) and hcnBC (for HCN production). Most biocontrol pseudomonads clustered separately from phytopathogenic proteobacteria, including pathogenic pseudomonads, in the hrcN tree. The exception was strain KD, which clustered with phytopathogenic pseudomonads, such as Pseudomonas syringae, suggesting that hrcN was acquired from the latter species. Indeed, strain KD (unlike strain SBW25) displayed the same organization of the hrpJ operon, which contains hrcN, as P. syringae. These results indicate that the occurrence of hrcN in most biocontrol pseudomonads is not the result of recent horizontal gene transfer from phytopathogenic bacteria, although such transfer might have occurred for a minority of biocontrol strains.

scholarly journals PopF1 and PopF2, Two Proteins Secreted by the Type III Protein Secretion System of Ralstonia solanacearum, Are Translocators Belonging to the HrpF/NopX Family

2006 ◽  
Vol 188 (13) ◽  
pp. 4903-4917 ◽  
Author(s):  
Damien Meyer ◽  
Sébastien Cunnac ◽  
Mareva Guéneron ◽  
Céline Declercq ◽  
Frédérique Van Gijsegem ◽  
...  
Keyword(s):  
Protein Secretion ◽  
Ralstonia Solanacearum ◽  
Xanthomonas Campestris ◽  
Pathogenic Bacteria ◽  
Secretion System ◽  
Effector Proteins ◽  
Experimental Conditions ◽  
Type Iii ◽  
Protein Secretion System ◽  
Type Iii Protein Secretion

ABSTRACT Ralstonia solanacearum GMI1000 is a gram-negative plant pathogen which contains an hrp gene cluster which codes for a type III protein secretion system (TTSS). We identified two novel Hrp-secreted proteins, called PopF1 and PopF2, which display similarity to one another and to putative TTSS translocators, HrpF and NopX, from Xanthomonas spp. and rhizobia, respectively. They also show similarities with TTSS translocators of the YopB family from animal-pathogenic bacteria. Both popF1 and popF2 belong to the HrpB regulon and are required for the interaction with plants, but PopF1 seems to play a more important role in virulence and hypersensitive response (HR) elicitation than PopF2 under our experimental conditions. PopF1 and PopF2 are not necessary for the secretion of effector proteins, but they are required for the translocation of AvrA avirulence protein into tobacco cells. We conclude that PopF1 and PopF2 are type III translocators belonging to the HrpF/NopX family. The hrpF gene of Xanthomonas campestris pv. campestris partially restored HR-inducing ability to popF1 popF2 mutants of R. solanacearum, suggesting that translocators of R. solanacearum and Xanthomonas are functionally conserved. Finally, R. solanacearum strain UW551, which does not belong to the same phylotype as GMI1000, also possesses two putative translocator proteins. However, although one of these proteins is clearly related to PopF1 and PopF2, the other seems to be different and related to NopX proteins, thus showing that translocators might be variable in R. solanacearum.

scholarly journals ClpXP Protease Controls Expression of the Type III Protein Secretion System through Regulation of RpoS and GrlR Levels in Enterohemorrhagic Escherichia coli

2005 ◽  
Vol 187 (12) ◽  
pp. 4086-4094 ◽  
Author(s):  
Sunao Iyoda ◽  
Haruo Watanabe
Keyword(s):  
Escherichia Coli ◽  
Protein Secretion ◽  
Deletion Mutant ◽  
Regulatory Gene ◽  
Secretion System ◽  
Enterohemorrhagic Escherichia Coli ◽  
Multicopy Plasmid ◽  
Type Iii ◽  
Protein Secretion System ◽  
Type Iii Protein Secretion

ABSTRACT Expression of the type III protein secretion system (TTSS), encoded in the locus of enterocyte effacement (LEE) of enterohemorrhagic Escherichia coli (EHEC), has been shown to be controlled by various regulators. In a search for additional regulatory genes, we identified a DNA fragment containing clpX and clpP that has a positive regulatory effect on LEE expression in EHEC O157. The expression of LEE-encoded Esp proteins was significantly reduced in a clpXP deletion mutant. Deletion of grlR, a negative regulatory gene within LEE, markedly increased LEE expression even in the clpXP mutant. To verify the regulatory mechanism of GrlR expression, a chromosomal epitope-tagged allele of grlR (grlR-FLAG) was constructed. GrlR-FLAG expression was increased significantly in the clpXP deletion mutant, suggesting that the GrlR level is under the control of ClpXP, and this regulation is critical for the ClpXP-dependent expression of LEE in EHEC. Deletion of rpoS, the gene encoding a stationary-phase-inducing sigma factor that is a substrate for ClpXP protease, partially restored LEE expression in the clpXP mutant. A multicopy plasmid carrying rpoS strongly repressed expression of Esp proteins, suggesting that positive regulation by ClpXP is partially mediated through a negative effect of RpoS on LEE expression. We also found that rpoS deletion induces transcription of pchA, which encodes one of the positive regulators for LEE expression in EHEC. These results suggest that ClpXP controls expression of LEE through the regulation of RpoS and GrlR levels in EHEC.

scholarly journals Type III Protein Secretion Is Associated with Death in Lower Respiratory and SystemicPseudomonas aeruginosaInfections

2001 ◽  
Vol 183 (12) ◽  
pp. 1767-1774 ◽  
Author(s):  
Arup Roy‐Burman ◽  
Richard H. Savel ◽  
Sara Racine ◽  
Britta L. Swanson ◽  
Neelambika S. Revadigar ◽  
...  
Keyword(s):  
Protein Secretion ◽  
Type Iii ◽  
Type Iii Protein Secretion

scholarly journals The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation

2014 ◽  
Vol 14 (1) ◽  
pp. 96 ◽  
Author(s):  
Tamara Zimaro ◽  
Ludivine Thomas ◽  
Claudius Marondedze ◽  
Germán G Sgro ◽  
Cecilia G Garofalo ◽  
...  
Keyword(s):  
Protein Secretion ◽  
Biofilm Formation ◽  
Secretion System ◽  
Xanthomonas Citri ◽  
Type Iii ◽  
Protein Secretion System ◽  
Type Iii Protein Secretion
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