scholarly journals Repair of transposable phage Mu DNA insertions begins only when theE. colireplisome collides with the transpososome

2015 ◽  
Vol 97 (4) ◽  
pp. 746-758 ◽  
Author(s):  
Sooin Jang ◽  
Rasika M. Harshey
Keyword(s):  
Phage Mu

The molecular cloning of the immunity gene of phage Mu

Gene ◽  
1977 ◽  
Vol 2 (5-6) ◽  
pp. 263-271 ◽  
Author(s):  
David Zipser ◽  
Phyllis Moses ◽  
Regine Kahmann ◽  
Dietmar Kamp
Keyword(s):  
Molecular Cloning ◽  
Immunity Gene ◽  
Phage Mu

Mapping of the modification function of temperate phage Mu-1

1980 ◽  
Vol 177 (2) ◽  
pp. 351-353 ◽  
Author(s):  
Ariane Toussaint ◽  
Lucie Desmet ◽  
Michel Faelen
Keyword(s):  
Temperate Phage ◽  
Phage Mu

Phage Mu

1988 ◽  
pp. 193-234 ◽  
Author(s):  
Rasika M. Harshey
Keyword(s):  
Phage Mu

Phage Mu ☆

2017 ◽  
Author(s):  
M.M. Howe ◽  
M.L. Pato
Keyword(s):  
Phage Mu

scholarly journals Baseplate assembly of phage Mu: Defining the conserved core components of contractile-tailed phages and related bacterial systems

2016 ◽  
Vol 113 (36) ◽  
pp. 10174-10179 ◽  
Author(s):  
Carina R. Büttner ◽  
Yingzhou Wu ◽  
Karen L. Maxwell ◽  
Alan R. Davidson
Keyword(s):  
Type Vi Secretion System ◽  
Secretion Systems ◽  
Phage Mu ◽  
Self Defense ◽  
Type Vi Secretion ◽  
Essential Components ◽  
Core Components ◽  
Contractile Tail ◽  
Equivalent Structure ◽  
Conserved Core

Contractile phage tails are powerful cell puncturing nanomachines that have been co-opted by bacteria for self-defense against both bacteria and eukaryotic cells. The tail of phage T4 has long served as the paradigm for understanding contractile tail-like systems despite its greater complexity compared with other contractile-tailed phages. Here, we present a detailed investigation of the assembly of a “simple” contractile-tailed phage baseplate, that of Escherichia coli phage Mu. By coexpressing various combinations of putative Mu baseplate proteins, we defined the required components of this baseplate and delineated its assembly pathway. We show that the Mu baseplate is constructed through the independent assembly of wedges that are organized around a central hub complex. The Mu wedges are comprised of only three protein subunits rather than the seven found in the equivalent structure in T4. Through extensive bioinformatic analyses, we found that homologs of the essential components of the Mu baseplate can be identified in the majority of contractile-tailed phages and prophages. No T4-like prophages were identified. The conserved simple baseplate components were also found in contractile tail-derived bacterial apparatuses, such as type VI secretion systems, Photorhabdus virulence cassettes, and R-type tailocins. Our work highlights the evolutionary connections and similarities in the biochemical behavior of phage Mu wedge components and the TssF and TssG proteins of the type VI secretion system. In addition, we demonstrate the importance of the Mu baseplate as a model system for understanding bacterial phage tail-derived systems.

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