scholarly journals TheLeishmania donovanichaperone cyclophilin 40 is essential for intracellular infection independent of its stage-specific phosphorylation status

2014 ◽  
Vol 93 (1) ◽  
pp. 80-97 ◽  
Author(s):  
Wai-Lok Yau ◽  
Pascale Pescher ◽  
Andrea MacDonald ◽  
Sonia Hem ◽  
Dorothea Zander ◽  
...  
Keyword(s):  
Intracellular Infection ◽  
Cyclophilin 40

scholarly journals Development of a Fluorescent Tool for Studying Legionella bozemanae Intracellular Infection

2021 ◽  
Vol 9 (2) ◽  
pp. 379
Author(s):  
Breanne M. Head ◽  
Christopher I. Graham ◽  
Teassa MacMartin ◽  
Yoav Keynan ◽  
Ann Karen C. Brassinga
Keyword(s):  
Growth Kinetics ◽  
Legionella Pneumophila ◽  
Fluorescent Protein ◽  
Disease Incidence ◽  
Acanthamoeba Castellanii ◽  
Infection Model ◽  
Intracellular Infection ◽  
Green Fluorescent

Legionnaires’ disease incidence is on the rise, with the majority of cases attributed to the intracellular pathogen, Legionella pneumophila. Nominally a parasite of protozoa, L. pneumophila can also infect alveolar macrophages when bacteria-laden aerosols enter the lungs of immunocompromised individuals. L. pneumophila pathogenesis has been well characterized; however, little is known about the >25 different Legionella spp. that can cause disease in humans. Here, we report for the first time a study demonstrating the intracellular infection of an L. bozemanae clinical isolate using approaches previously established for L. pneumophila investigations. Specifically, we report on the modification and use of a green fluorescent protein (GFP)-expressing plasmid as a tool to monitor the L. bozemanae presence in the Acanthamoeba castellanii protozoan infection model. As comparative controls, L. pneumophila strains were also transformed with the GFP-expressing plasmid. In vitro and in vivo growth kinetics of the Legionella parental and GFP-expressing strains were conducted followed by confocal microscopy. Results suggest that the metabolic burden imposed by GFP expression did not impact cell viability, as growth kinetics were similar between the GFP-expressing Legionella spp. and their parental strains. This study demonstrates that the use of a GFP-expressing plasmid can serve as a viable approach for investigating Legionella non-pneumophila spp. in real time.

scholarly journals 010 Polycytotoxic T cells protect against intracellular infection

2016 ◽  
Vol 136 (5) ◽  
pp. S2
Author(s):  
S.J. Balin ◽  
S. Stenger ◽  
M. Pellegrni ◽  
M. Ochoa ◽  
M. Schenk ◽  
...  
Keyword(s):  
T Cells ◽  
Intracellular Infection

scholarly journals Cell-specific Activation of Nuclear Factor-κB by the ParasiteTrypanosoma cruziPromotes Resistance to Intracellular Infection

2000 ◽  
Vol 11 (1) ◽  
pp. 153-160 ◽  
Author(s):  
Belinda S. Hall ◽  
Winnie Tam ◽  
Ranjan Sen ◽  
Miercio E. A. Pereira
Keyword(s):  
Mammalian Cells ◽  
Nuclear Translocation ◽  
Nuclear Factor Κb ◽  
Microbial Pathogens ◽  
Tissue Tropism ◽  
Nuclear Factor ◽  
Infection Level ◽  
Direct Role ◽  
Intracellular Infection

The transcription factor nuclear factor-κB (NF-κB) is central to the innate and acquired immune response to microbial pathogens, coordinating cellular responses to the presence of infection. Here we demonstrate a direct role for NF-κB activation in controlling intracellular infection in nonimmune cells. Trypanosoma cruzi is an intracellular parasite of mammalian cells with a marked preference for infection of myocytes. The molecular basis for this tissue tropism is unknown. Trypomastigotes, the infectious stage of T. cruzi, activate nuclear translocation and DNA binding of NF-κB p65 subunit and NF-κB-dependent gene expression in epithelial cells, endothelial cells, and fibroblasts. Inactivation of epithelial cell NF-κB signaling by inducible expression of the inhibitory mutant IκBaM significantly enhances parasite invasion.T. cruzi do not activate NF-κB in cells derived from skeletal, smooth, or cardiac muscle, despite the ability of these cells to respond to tumor necrosis factor-α with NF-κB activation. The in vitro infection level in these muscle-derived cells is more than double that seen in the other cell types tested. Therefore, the ability of T. cruzi to activate NF-κB correlates inversely with susceptibility to infection, suggesting that NF-κB activation is a determinant of the intracellular survival and tissue tropism ofT. cruzi.

scholarly journals Human cyclophilin 40 is a heat shock protein that exhibits altered intracellular localization following heat shock

2001 ◽  
Vol 6 (1) ◽  
pp. 59 ◽  
Author(s):  
Peter J. Mark ◽  
Bryan K. Ward ◽  
Premlata Kumar ◽  
Hooshang Lahooti ◽  
Rodney F. Minchin ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Intracellular Localization ◽  
Cyclophilin 40

scholarly journals Tryptophan Catabolism During Intracellular Infection

2012 ◽  
Vol 205 (10) ◽  
pp. 1617-1618
Author(s):  
Sebastian Schroecksnadel ◽  
Katharina Kurz ◽  
Guenter Weiss ◽  
Dietmar Fuchs
Keyword(s):  
Intracellular Infection ◽  
Tryptophan Catabolism

scholarly journals Systematic, multiparametric analysis of Mycobacterium tuberculosis intracellular infection offers insight into coordinated virulence

2017 ◽  
Vol 13 (5) ◽  
pp. e1006363 ◽  
Author(s):  
Amy K. Barczak ◽  
Roi Avraham ◽  
Shantanu Singh ◽  
Samantha S. Luo ◽  
Wei Ran Zhang ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Intracellular Infection ◽  
Multiparametric Analysis ◽  
Insight Into
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