scholarly journals Streptococcus pneumoniaeR6 interspecies transformation: genetic analysis of penicillin resistance determinants and genome-wide recombination events

2012 ◽  
Vol 86 (3) ◽  
pp. 692-706 ◽  
Author(s):  
Julia Sauerbier ◽  
Patrick Maurer ◽  
Martin Rieger ◽  
Regine Hakenbeck
Keyword(s):  
Genetic Analysis ◽  
Penicillin Resistance ◽  
Resistance Determinants ◽  
Genome Wide

scholarly journals Population genetic analysis and phylogeny reconstruction in Eucalyptus (Myrtaceae) using high-throughput, genome-wide genotyping

2011 ◽  
Vol 59 (1) ◽  
pp. 206-224 ◽  
Author(s):  
Dorothy A. Steane ◽  
Dean Nicolle ◽  
Carolina P. Sansaloni ◽  
César D. Petroli ◽  
Jason Carling ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
High Throughput ◽  
Population Genetic ◽  
Phylogeny Reconstruction ◽  
Population Genetic Analysis ◽  
Genome Wide

SNP discovery by amplicon sequencing and multiplex SNP genotyping in the allopolyploid species Brassica napusThis article is one of a selection of papers from the conference “Exploiting Genome-wide Association in Oilseed Brassicas: a model for genetic improvement of major OECD crops for sustainable farming”.

Genome ◽  
2010 ◽  
Vol 53 (11) ◽  
pp. 948-956 ◽  
Author(s):  
G. Durstewitz ◽  
A. Polley ◽  
J. Plieske ◽  
H. Luerssen ◽  
E. M. Graner ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Oilseed Rape ◽  
Expressed Sequence Tag ◽  
Diploid Species ◽  
Amplicon Sequencing ◽  
Snp Markers ◽  
Snp Analysis ◽  
Genome Wide ◽  
A Genome ◽  
Illumina Goldengate

Oilseed rape ( Brassica napus ) is an allotetraploid species consisting of two genomes, derived from B. rapa (A genome) and B. oleracea (C genome). The presence of these two genomes makes single nucleotide polymorphism (SNP) marker identification and SNP analysis more challenging than in diploid species, as for a given locus usually two versions of a DNA sequence (based on the two ancestral genomes) have to be analyzed simultaneously during SNP identification and analysis. One hundred amplicons derived from expressed sequence tag (ESTs) were analyzed to identify SNPs in a panel of oilseed rape varieties and within two sister species representing the ancestral genomes. A total of 604 SNPs were identified, averaging one SNP in every 42 bp. It was possible to clearly discriminate SNPs that are polymorphic between different plant varieties from SNPs differentiating the two ancestral genomes. To validate the identified SNPs for their use in genetic analysis, we have developed Illumina GoldenGate assays for some of the identified SNPs. Through the analysis of a number of oilseed rape varieties and mapping populations with GoldenGate assays, we were able to identify a number of different segregation patterns in allotetraploid oilseed rape. The majority of the identified SNP markers can be readily used for genetic mapping, showing that amplicon sequencing and Illumina GoldenGate assays can be used to reliably identify SNP markers in tetraploid oilseed rape and to convert them into successful SNP assays that can be used for genetic analysis.

scholarly journals Revisiting demographic processes in cattle with genome-wide population genetic analysis

2015 ◽  
Vol 6 ◽  
Author(s):  
Pablo Orozco-terWengel ◽  
Mario Barbato ◽  
Ezequiel Nicolazzi ◽  
Filippo Biscarini ◽  
Marco Milanesi ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Population Genetic ◽  
Population Genetic Analysis ◽  
Genome Wide ◽  
Demographic Processes ◽  
Wide Population

Transduction of penicillinase production in Staphylococcus epidermidis and nature of the genetic determinant

1979 ◽  
Vol 25 (4) ◽  
pp. 508-511 ◽  
Author(s):  
W. C. Olson Jr. ◽  
J. T. Parisi ◽  
P. A. Totten ◽  
J. N. Baldwin
Keyword(s):  
Ethidium Bromide ◽  
Staphylococcus Epidermidis ◽  
Tetracycline Resistance ◽  
Recipient Strain ◽  
Penicillin Resistance ◽  
Agarose Gel ◽  
Donor Strain ◽  
Genetic Determinant ◽  
Resistance Determinants ◽  
Curing Agents

Four strains of Staphylococcus epidermidis from clinical sources were capable of serving as donors for the transduction of either penicillinase production, ethidium bromide resistance, or tetracycline resistance. Three typing phages served as transducing phages and, depending upon the combination of transducing phage, donor strain, and recipient strain, the rates of transduction ranged between 10−5 and 10−9. In one strain, cotransduction of penicillinase production and ethidium bromide resistance was observed. Although ultraviolet irradiation kinetics indicated that both the tetracycline resistance and the penicillin resistance determinants were located on plasmids, only resistance to tetracycline could be eliminated by growth in the presence of curing agents or at elevated temperature. However, evidence was obtained by agarose gel electrophoretic studies that both the tetracycline resistance and the penicillin resistance determinants are located on separate plasmids in this organism.

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