Thioredoxin deficiency exacerbates vascular dysfunction during diet‐induced obesity in small mesenteric artery in mice

2020 ◽  
Author(s):  
Shannon Dunn ◽  
Robert H. Hilgers ◽  
Kumuda C. Das
Keyword(s):  
Mesenteric Artery ◽  
Vascular Dysfunction ◽  
Diet Induced Obesity ◽  
Small Mesenteric Artery

Depletion of microglia mitigates cerebrovascular dysfunction in diet-induced obesity mice

2021 ◽  
Author(s):  
Qing Shen ◽  
Guo Zhang
Keyword(s):  
Basilar Artery ◽  
Vascular Dysfunction ◽  
Underlying Mechanism ◽  
Diet Induced Obesity ◽  
Cerebrovascular Function ◽  
Cerebrovascular Dysfunction ◽  
Normal Body Weight ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide ◽  
The Brain

Obesity is frequently associated with cerebrovascular dysfunction, however, the underlying mechanism remains less well understood. In this study, by using pharmacological approaches, we show that neuroinflammation involving microglia plays an important role in obesity-related cerebrovascular dysfunction. PLX3397 treatment, which leads to depletion of microglia, reduced the wall thickness and collagen deposition in the basilar artery of diet-induced obesity (DIO) mice. Besides, the phosphorylation of endothelial nitric oxide synthase (eNOS) at Ser1177 was enhanced, suggesting improved endothelial function of the basilar artery. The wire myography data show that acetylcholine-elicited relaxation of basilar artery isolated from DIO mice was improved after the treatment with PLX3397. Moreover, our data demonstrate that brain administration of IL-18 impaired cerebrovascular function in mice with normal body weight. Together, these data suggest that neuroinflammation involving microglia is important in obesity-related vascular dysfunction in the brain.

Nanomolar ouabain augments caffeine-evoked contractions in rat arteries

1993 ◽  
Vol 265 (5) ◽  
pp. C1443-C1448 ◽  
Author(s):  
D. N. Weiss ◽  
D. J. Podberesky ◽  
J. Heidrich ◽  
M. P. Blaustein
Keyword(s):  
Dose Response ◽  
Mesenteric Artery ◽  
Response Curve ◽  
Dose Response Curve ◽  
Response Curves ◽  
Low Concentrations ◽  
Vascular Responsiveness ◽  
Subunit Isoforms ◽  
Evoked Contractions ◽  
Small Mesenteric Artery

Chronic parenteral administration of ouabain to normal rats raises plasma ouabain concentrations to low nanomolar levels and induces hypertension [C. M. Yuan, P. Manunta, J. M. Hamlyn, S. W. Chen, E. Bohen, J. Yeun, F. J. Haddy, and M. B. Pamnani. Hypertension 22: 178-187, 1993 and see also M. P. Blaustein. Am. J. Physiol. 264 (Cell Physiol. 33): C1367-C1387, 1993]. To determine whether rat arteries are sensitive to these low ouabain levels, we tested the effects of various ouabain concentrations on caffeine-evoked contractions (CEC) in rat aortic and small mesenteric artery rings. CEC amplitude was used as a measure of the sarcoplasmic reticulum (SR) Ca2+ content. Ouabain increased CEC in aortic as well as mesenteric artery rings, but the effects in the aorta were difficult to quantitate because the CEC were often oscillatory. Mesenteric artery, under control conditions and after sensitization with 10-30 nM phenylephrine (PE), exhibited biphasic ouabain dose-CEC response curves. Low concentrations of ouabain (0.1-10 nM) caused small significant increases in CEC, but a further effect was observed only with > or = 10 microM ouabain. PE shifted the ouabain dose-response curve toward lower ouabain concentrations; conversely, ouabain shifted the PE dose-response curve toward lower PE concentrations. It appears that nanomolar concentrations of ouabain can influence vascular responsiveness to vasoconstrictors. We conclude that rat vascular smooth muscle contains both high- and low-affinity ouabain receptors, possibly corresponding to Na+ pumps with alpha 3- and alpha 1-subunit isoforms, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Catalase has negligible inhibitory effects on endothelium-dependent relaxations in mouse isolated aorta and small mesenteric artery

2003 ◽  
Vol 140 (7) ◽  
pp. 1193-1200 ◽  
Author(s):  
Anthie Ellis ◽  
Malarvannan Pannirselvam ◽  
Todd J Anderson ◽  
Chris R Triggle
Keyword(s):  
Mesenteric Artery ◽  
Inhibitory Effects ◽  
Isolated Aorta ◽  
Small Mesenteric Artery
Sign in / Sign up

Export Citation Format

Share Document