scholarly journals Evolutionary trade‐offs of insecticide resistance — The fitness costs associated with target‐site mutations in the nAChR of Drosophila melanogaster

2020 ◽  
Vol 29 (14) ◽  
pp. 2661-2675 ◽  
Author(s):  
Rafael A. Homem ◽  
Bliss Buttery ◽  
Ewan Richardson ◽  
Yao Tan ◽  
Linda M. Field ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Insecticide Resistance ◽  
Target Site ◽  
Fitness Costs ◽  
Trade Offs

Fitness costs associated with spinetoram resistance in Spodoptera frugiperda is driven by host plants

2021 ◽  
Author(s):  
Rubens Hideo Kanno ◽  
Aline Guidolin ◽  
Fernando Padovez ◽  
Juliana Rodrigues ◽  
Celso Omoto
Keyword(s):  
Insecticide Resistance ◽  
Spodoptera Frugiperda ◽  
Host Plants ◽  
Agricultural Landscape ◽  
Growth Parameters ◽  
Resistance Management ◽  
Population Increase ◽  
Relative Fitness ◽  
Fitness Costs ◽  
Cotton Plants

Insecticide resistance is usually associated with fitness costs. The magnitude of fitness costs is affected by environmental and ecological factors. Here, we explored how host plants could affect fitness costs associated with insecticide resistance. Initially, spinetoram-resistant (RR) and susceptible (SS) strains of Spodoptera frugiperda were selected using F2 screen from a population collected in Sao Desiderio, Bahia State, Brazil in 2018. Besides de RR and SS strains, fitness costs were also assessed for a heterozygous strain (RS). Life-history traits were evaluated to estimate population growth parameters of neonate larvae of each strain fed on corn, soybean and cotton plants. Compared to the SS strain, the relative fitness of the RR strain, based on intrinsic rate of population increase, was 1.06, 0.84 and 0.67 on plants of corn, soybean and cotton respectively. The relative fitness of the RS strain was similar to the SS strain regardless the host plant, suggesting a recessive fitness cost. No differences were found between the strains fed on corn plants. The larval development time was greater for RR strain fed on soybean and cotton plants compared to RS and SS strain. Low survival rate and fecundity of the RR strain were found when larvae fed on plants of soybean and cotton. The results of this study demonstrated that fitness costs of spinetoram resistance in S. frugiperda depend strongly on the host plants that S. frugiperda larvae fed on. Such information can be used to design resistance management strategies considering the host plants of the agricultural landscape. Keywords: fall armyworm; spinosyns; insect resistance management; relative fitness.

scholarly journals Transcriptomic analysis of insecticide resistance in the lymphatic filariasis vectorCulex quinquefasciatus

2019 ◽  
Author(s):  
Walter Fabricio Silva Martins ◽  
Craig Stephen Wilding ◽  
Alison Taylor Isaacs ◽  
Emily Joy Rippon ◽  
Karine Megy ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Lymphatic Filariasis ◽  
Fold Increase ◽  
Target Site ◽  
Detoxification Enzymes ◽  
Site Mutation ◽  
Public Health Importance ◽  
Metabolic Resistance ◽  
Vector Borne Diseases ◽  
Bendiocarb Resistance

ABSTRACTCulex quinquefasciatusplays an important role in transmission of vector-borne diseases of public health importance, including lymphatic filariasis (LF), as well as many arboviral diseases. Currently, efforts to tackleC. quinquefasciatusvectored diseases are based on either mass drug administration (MDA) for LF, or insecticide-based interventions. Widespread and intensive insecticide usage has resulted in increased resistance in mosquito vectors, includingC. quinquefasciatus. Herein, the transcriptome profile of Ugandan bendiocarb-resistantC. quinquefasciatuswas explored to identify candidate genes associated with insecticide resistance. Resistance to bendiocarb in exposed mosquitoes was marked, with 2.04% mortality following 1h exposure and 58.02% after 4h. Genotyping of the G119SAce-1target site mutation detected a highly significant association (p<0.0001; OR=25) between resistance andAce1-119S. However, synergist assays using the P450 inhibitor PBO or the esterase inhibitor TPP resulted in markedly increased mortality (to ≈80%), suggesting a role of metabolic resistance in the resistance phenotype. Using a novel, custom 60K whole-transcriptome microarray 16 genes significantly overexpressed in resistant mosquitoes were detected, with the P450Cyp6z18showing the highest differential gene expression (>8-fold increase vs unexposed controls). These results provide evidence that bendiocarb-resistance in UgandanC. quinquefasciatusis mediated by both target-site mechanisms and over-expression of detoxification enzymes.

Complex Trade-Offs and the Evolution of Starvation Resistance in Drosophila melanogaster

Evolution ◽  
1996 ◽  
Vol 50 (2) ◽  
pp. 753 ◽  
Author(s):  
Adam K. Chippindale ◽  
Terence J. F. Chu ◽  
Michael R. Rose
Keyword(s):  
Drosophila Melanogaster ◽  
Starvation Resistance ◽  
Trade Offs

scholarly journals Multiple insecticide resistance target sites in adult field strains of An. gambiae (s.l.) from southeastern Senegal

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
El hadji Diouf ◽  
El hadji Amadou Niang ◽  
Badara Samb ◽  
Cheikh Tidiane Diagne ◽  
Mbaye Diouf ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Indoor Residual Spraying ◽  
National Malaria Control Programme ◽  
Control Programme ◽  
Molecular Techniques ◽  
Target Site ◽  
Malaria Control Programme ◽  
Insecticide Susceptibility ◽  
High Coverage ◽  
Pyrethroid Insecticides

Abstract Background High coverage of long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) are the cornerstones of vector control strategy in Senegal where insecticide resistance by the target vectors species is a great of concern. This study explores insecticide susceptibility profile and target-site mutations mechanisms within the Anophelesgambiae complex in southeastern Senegal. Methods Larvae of Anopheles spp. were collected in two sites from southeastern Senegal Kedougou and Wassadou/Badi in October and November 2014, and reared until adult emergence. Wild F0 adult mosquitoes were morphologically identified to species. Susceptibility of 3–5-day-old An. gambiae (s.l.) samples to 11 insecticides belonging to the four insecticide classes was assessed using the WHO insecticide susceptibility bioassays. Tested samples were identified using molecular techniques and insecticide resistance target-site mutations (kdr, ace-1 and rdl) were determined. Results A total of 3742 An.gambiae (s.l.) were exposed to insecticides (2439 from Kedougou and 1303 from Wassadou-Badi). Tests with pyrethroid insecticides and DDT showed high level of resistance in both Kedougou and Wassadou/Badi. Resistance to pirimiphos-methyl and malathion was not detected while resistance to bendoicarb and fenitrothion was confirmed in Kedougou. Of the 745 specimens of An.gambiae (s.l.) genotyped, An.gambiae (s.s.) (71.6%) was the predominant species, followed by An.arabiensis (21.7%), An.coluzzii (6.3%) and hybrids (An. gambiae (s.s.)/An.coluzzii; 0.4%). All target site mutations investigated (Vgsc-1014F, Vgsc-1014S, Ace-1 and Rdl) were found at different frequencies in the species of the Anophelesgambiae complex. Vgsc-1014F mutation was more frequent in An.gambiae (s.s.) and An.coluzzii than An.arabiensis. Vgsc-1014S was present in An.gambiae (s.l.) populations in Wassadou but not in Kedougou. Ace-1 and rdl mutations were more frequent in An.gambiae (s.s.) in comparison to An.arabiensis and An.coluzzii. Conclusions Resistance to all the four insecticide classes tested was detected in southeastern Senegal as well as all target site mutations investigated were found. Data will be used by the national Malaria Control Programme.

Sign in / Sign up

Export Citation Format

Share Document