scholarly journals Causes and consequences of large clonal assemblies in a poplar hybrid zone

2016 ◽  
Vol 25 (21) ◽  
pp. 5330-5344 ◽  
Author(s):  
David Macaya-Sanz ◽  
Myriam Heuertz ◽  
Dorothea Lindtke ◽  
Giovanni G. Vendramin ◽  
Christian Lexer ◽  
...  
Keyword(s):  
Hybrid Zone ◽  
Poplar Hybrid

scholarly journals Fitness dynamics within a poplar hybrid zone: II . Impact of exotic sex on native poplars in an urban jungle

2014 ◽  
Vol 4 (10) ◽  
pp. 1876-1889 ◽  
Author(s):  
Amanda D. Roe ◽  
Chris J.K. MacQuarrie ◽  
Marie‐Claude Gros‐Louis ◽  
J. Dale Simpson ◽  
Josyanne Lamarche ◽  
...  
Keyword(s):  
Hybrid Zone ◽  
Poplar Hybrid ◽  
Zone Ii

scholarly journals Fitness dynamics within a poplar hybrid zone: I. P rezygotic and postzygotic barriers impacting a native poplar hybrid stand

2014 ◽  
Vol 4 (9) ◽  
pp. 1629-1647 ◽  
Author(s):  
Amanda D. Roe ◽  
Chris J. K. MacQuarrie ◽  
Marie‐Claude Gros‐Louis ◽  
J. Dale Simpson ◽  
Josyanne Lamarche ◽  
...  
Keyword(s):  
Hybrid Zone ◽  
Postzygotic Barriers ◽  
Poplar Hybrid

RELATIVE ABUNDANCE AND THE SPECIES-SPECIFIC REINFORCEMENT OF MALE MATING PREFERENCE IN THE CHRYSOCHUS (COLEOPTERA: CHRYSOMELIDAE) HYBRID ZONE

Evolution ◽  
2005 ◽  
Vol 59 (12) ◽  
pp. 2639 ◽  
Author(s):  
Merrill A. Peterson ◽  
Barbara M. Honchak ◽  
Stefanie E. Locke ◽  
Timothy E. Beeman ◽  
Jessica Mendoza ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Hybrid Zone ◽  
Mating Preference ◽  
Male Mating ◽  
Species Specific

Behavioral Stabilization of a Baboon Hybrid Zone

1975 ◽  
Vol 109 (970) ◽  
pp. 701-712 ◽  
Author(s):  
Stephen A. Gabow
Keyword(s):  
Hybrid Zone

scholarly journals X-Linked Signature of Reproductive Isolation in Humans is Mirrored in a Howler Monkey Hybrid Zone

2020 ◽  
Vol 111 (5) ◽  
pp. 419-428 ◽  
Author(s):  
Marcella D Baiz ◽  
Priscilla K Tucker ◽  
Jacob L Mueller ◽  
Liliana Cortés-Ortiz
Keyword(s):  
Reproductive Isolation ◽  
X Chromosome ◽  
Hybrid Zone ◽  
Sex Chromosome ◽  
Genomic Sequence ◽  
Alouatta Palliata ◽  
Model Systems ◽  
Howler Monkey ◽  
Autosomal Snps ◽  
Outlier Region

Abstract Reproductive isolation is a fundamental step in speciation. While sex chromosomes have been linked to reproductive isolation in many model systems, including hominids, genetic studies of the contribution of sex chromosome loci to speciation for natural populations are relatively sparse. Natural hybrid zones can help identify genomic regions contributing to reproductive isolation, like hybrid incompatibility loci, since these regions exhibit reduced introgression between parental species. Here, we use a primate hybrid zone (Alouatta palliata × Alouatta pigra) to test for reduced introgression of X-linked SNPs compared to autosomal SNPs. To identify X-linked sequence in A. palliata, we used a sex-biased mapping approach with whole-genome re-sequencing data. We then used genomic cline analysis with reduced-representation sequence data for parental A. palliata and A. pigra individuals and hybrids (n = 88) to identify regions with non-neutral introgression. We identified ~26 Mb of non-repetitive, putatively X-linked genomic sequence in A. palliata, most of which mapped collinearly to the marmoset and human X chromosomes. We found that X-linked SNPs had reduced introgression and an excess of ancestry from A. palliata as compared to autosomal SNPs. One outlier region with reduced introgression overlaps a previously described “desert” of archaic hominin ancestry on the human X chromosome. These results are consistent with a large role for the X chromosome in speciation across animal taxa and further, suggest shared features in the genomic basis of the evolution of reproductive isolation in primates.

scholarly journals Evaluating the Efficiency of gRNAs in CRISPR/Cas9 Mediated Genome Editing in Poplars

2019 ◽  
Vol 20 (15) ◽  
pp. 3623 ◽  
Author(s):  
Tobias Bruegmann ◽  
Khira Deecke ◽  
Matthias Fladung
Keyword(s):  
Genome Editing ◽  
Gene Editing ◽  
Constitutive Expression ◽  
Gc Content ◽  
Seed Region ◽  
Research Topics ◽  
Target Region ◽  
Cas9 Nuclease ◽  
Different Types ◽  
Poplar Hybrid

CRISPR/Cas9 has become one of the most promising techniques for genome editing in plants and works very well in poplars with an Agrobacterium-mediated transformation system. We selected twelve genes, including SOC1, FUL, and their paralogous genes, four NFP-like genes and TOZ19 for three different research topics. The gRNAs were designed for editing, and, together with a constitutively expressed Cas9 nuclease, transferred either into the poplar hybrid Populus × canescens or into P. tremula. The regenerated lines showed different types of editing and revealed several homozygous editing events which are of special interest in perennial species because of limited back-cross ability. Through a time series, we could show that despite the constitutive expression of the Cas9 nuclease, no secondary editing of the target region occurred. Thus, constitutive Cas9 expression does not seem to pose any risk to additional editing events. Based on various criteria, we obtained evidence for a relationship between the structure of gRNA and the efficiency of gene editing. In particular, the GC content, purine residues in the gRNA end, and the free accessibility of the seed region seemed to be highly important for genome editing in poplars. Based on our findings on nine different poplar genes, efficient gRNAs can be designed for future efficient editing applications in poplars.

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