Comparative landscape genetics of two river frog species occurring at different elevations on Mount Kilimanjaro

2014 ◽  
Vol 23 (20) ◽  
pp. 4989-5002 ◽  
Author(s):  
Giulia Zancolli ◽  
Mark-Oliver Rödel ◽  
Ingolf Steffan-Dewenter ◽  
Andrew Storfer
2017 ◽  
Vol 11 (1) ◽  

The Taal Volcano Protected Landscape (TVPL) encompasses a prehistoric volcano caldera that caters to many documented endemic species. Although regarded as a unique area with the potential to house a diverse ecological community, biodiversity research in TVPL is still found wanting. The present paper aims to provide baseline information and increase research interests on the herpetofaunal diversity of TVPL, in light of its many undocumented terrestrial faunal species. Twelve study sites within the municipalities of Tanauan, Mataasnakahoy, and Balete were visited during survey trips from May to November 2015. A combination of transect and opportunistic sampling techniques were utilized, with morphometric data and sexual maturity recorded for each specimen collected. This preliminary survey provided 24 newly documented species of amphibians and reptiles occurring within TVPL. A total 10 frog species (from families Bufonidae, Ceratobatrachidae, Microhylidae, Dicroglossidae, Ranidae, and Rhacophoridae) and 14 reptile species (from families Agamidae, Gekkonidae, Scincidae, Varanidae, Acrochordidae, Colubridae, Elapidae, and Tryonychidae) were documented. Of the reptiles recorded, 3 are endemic species and widespread throughout the Philippines: Gekko mindorensis, Hydrosaurus pustulatus, and Draco spilopterus. Also recorded were the Philippine endemic frogs Kaloula picta and Limnonectes woodworthi along with the Luzon endemics Platymantis mimulus and Varanus marmoratus. The species-effort curve of amphibians showed a distinct plateau whereas the species-effort curve of reptiles has shown an increasing trend suggesting that additional sampling efforts should be done in the area to further increase knowledge of the TVPL herpetofaunal diversity.


2021 ◽  
Vol 207 (1) ◽  
pp. 87-98
Author(s):  
Hiromitsu Awano ◽  
Masahiro Shirasaka ◽  
Takeshi Mizumoto ◽  
Hiroshi G. Okuno ◽  
Ikkyu Aihara

2014 ◽  
Vol 15 (3) ◽  
pp. 512-525 ◽  
Author(s):  
E. M. Kierepka ◽  
E. K. Latch
Keyword(s):  

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Zhongying Wang ◽  
Qixuan Wang ◽  
Hao Wu ◽  
Zhiwu Huang

Abstract Background Prestin (SLC26A5) is responsible for acute sensitivity and frequency selectivity in the vertebrate auditory system. Limited knowledge of prestin is from experiments using site-directed mutagenesis or domain-swapping techniques after the amino acid residues were identified by comparing the sequence of prestin to those of its paralogs and orthologs. Frog prestin is the only representative in amphibian lineage and the studies of it were quite rare with only one species identified. Results Here we report a new coding sequence of SLC26A5 for a frog species, Rana catesbeiana (the American bullfrog). In our study, the SLC26A5 gene of Rana has been mapped, sequenced and cloned successively using RNA-Seq. We measured the nonlinear capacitance (NLC) of prestin both in the hair cells of Rana’s inner ear and HEK293T cells transfected with this new coding gene. HEK293T cells expressing Rana prestin showed electrophysiological features similar to that of hair cells from its inner ear. Comparative studies of zebrafish, chick, Rana and an ancient frog species showed that chick and zebrafish prestin lacked NLC. Ancient frog’s prestin was functionally different from Rana. Conclusions We mapped and sequenced the SLC26A5 of the Rana catesbeiana from its inner ear cDNA using RNA-Seq. The Rana SLC26A5 cDNA was 2292 bp long, encoding a polypeptide of 763 amino acid residues, with 40% identity to mammals. This new coding gene could encode a functionally active protein conferring NLC to both frog HCs and the mammalian cell line. While comparing to its orthologs, the amphibian prestin has been evolutionarily changing its function and becomes more advanced than avian and teleost prestin.


PLoS ONE ◽  
2011 ◽  
Vol 6 (10) ◽  
pp. e25359 ◽  
Author(s):  
Patrick M. A. James ◽  
Dave W. Coltman ◽  
Brent W. Murray ◽  
Richard C. Hamelin ◽  
Felix A. H. Sperling

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