scholarly journals Peripheral phenotype and gene expression profiles of combined liver-kidney transplant patients

2015 ◽  
Vol 36 (3) ◽  
pp. 401-409 ◽  
Author(s):  
Erwan Dumontet ◽  
Richard Danger ◽  
Parsia A. Vagefi ◽  
Maria-Carlota Londoño ◽  
Annaïck Pallier ◽  
...  
Keyword(s):  
Gene Expression ◽  
Kidney Transplant ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Transplant Patients ◽  
Kidney Transplant Patients ◽  
Combined Liver Kidney Transplant

Increasing of the interferon-γ gene expression during polyomavirus BK infection in kidney transplant patients

2019 ◽  
Vol 129 ◽  
pp. 187-194 ◽  
Author(s):  
Neda Zareei ◽  
Hamid Reza Miri ◽  
Mohammad Hossein Karimi ◽  
Afsoon Afshari ◽  
Bita Geramizadeh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Kidney Transplant ◽  
Interferon Γ ◽  
Transplant Patients ◽  
Kidney Transplant Patients ◽  
Polyomavirus Bk

scholarly journals Acute and Chronic Changes in Gene Expression After CMV DNAemia in Kidney Transplant Recipients

2021 ◽  
Vol 12 ◽  
Author(s):  
Richard Ahn ◽  
Joanna Schaenman ◽  
Zachary Qian ◽  
Harry Pickering ◽  
Victoria Groysberg ◽  
...  
Keyword(s):  
Gene Expression ◽  
Kidney Transplant ◽  
Time Course ◽  
Cell Function ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Transplant Patients ◽  
Kidney Transplant Patients ◽  
Cmv Dnaemia

Cytomegalovirus (CMV) viremia continues to cause significant morbidity and mortality in kidney transplant patients with clinical complications including organ rejection and death. Whole blood gene expression dynamics in CMV viremic patients from onset of DNAemia through convalescence has not been well studied to date in humans. To evaluate how CMV infection impacts whole blood leukocyte gene expression over time, we evaluated a matched cohort of 62 kidney transplant recipients with and without CMV DNAemia using blood samples collected at multiple time points during the 12-month period after transplant. While transcriptomic differences were minimal at baseline between DNAemic and non-DNAemic patients, hundreds of genes were differentially expressed at the long-term timepoint, including genes enriching for pathways important for macrophages, interferon, and IL-8 signaling. Amongst patients with CMV DNAemia, the greatest amount of transcriptomic change occurred between baseline and 1-week post-DNAemia, with increase in pathways for interferon signaling and cytotoxic T cell function. Time-course gene set analysis of these differentially expressed genes revealed that most of the enriched pathways had a significant time-trend. While many pathways that were significantly down- or upregulated at 1 week returned to baseline-like levels, we noted that several pathways important in adaptive and innate cell function remained upregulated at the long-term timepoint after resolution of CMV DNAemia. Differential expression analysis and time-course gene set analysis revealed the dynamics of genes and pathways involved in the immune response to CMV DNAemia in kidney transplant patients. Understanding transcriptional changes caused by CMV DNAemia may identify the mechanism behind patient vulnerability to CMV reactivation and increased risk of rejection in transplant recipients and suggest protective strategies to counter the negative immunologic impact of CMV. These findings provide a framework to identify immune correlates for risk assessment and guiding need for extending antiviral prophylaxis.

scholarly journals Recurrent JAK3 Mutations in Post-Transplant Lymphoproliferative Disorder

Blood ◽  
2019 ◽  
Vol 134 (Supplement_1) ◽  
pp. 5226-5226
Author(s):  
Alexandra Butzmann ◽  
Diwash Jangam ◽  
Jyoti Kumar ◽  
Kaushik Sridhar ◽  
Benjamin A Pinsky ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Lymphoid Tissues ◽  
Hematopoietic Stem ◽  
Regulatory Pathways ◽  
Follicular Hyperplasia ◽  
Post Transplant ◽  
Transplant Patients ◽  
Recurrent Mutations

Post-transplant lymphoproliferative disorders (PTLD) are diverse in morphology, immunophenotype and clinical outcomes, occurring in immune compromised patients after hematopoietic stem cell transplantation (HCT) or single organ transplantation (SOT). Predicting the outcome of PTLDs is complex. In most of cases PTLD is driven by Epstein-Barr virus (EBV) infection, though the etiology of EBV negative cases is unknown. There have been only few studies investigating the mutational landscape and gene expression of PTLD. In our study we performed targeted DNA deep sequencing and RNA expression profiling (RNA-Seq) on the hematopoietic lymphoid tissues of 15 post-transplant patients with PTLD and 16 post-transplant patients with follicular hyperplasia (FH) after HCT or SOT. Recurrent mutations in JAK3 were identified in five of 16 PTLD cases and one of 16 follicular hyperplasia cases. We additionally identified mutations in the PIK3CD gene in four PTLD cases and NOTCH1 mutations in 2 PTLD and four FH cases. 17 other genes were commonly mutated in both groups. Gene expression profiles differed between both groups. Genes involved in regulating the immune system were unregulated in PTLD. We also saw a stronger expression of T-cells and a weaker expression of B-cells within the PTLD group when compared with FH. Our findings suggest an important role for immune regulatory pathways in the pathogenesis of PTLD, with the JAK/STAT pathway being a central recurrent key metabolic pathway. Figure Disclosures Ohgami: Agilent technologies: Other: received support/funding.

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