The impact of hepatic steatosis on liver regeneration after partial hepatectomy

2013 ◽  
Vol 33 (3) ◽  
pp. 469-475 ◽  
Author(s):  
Petra G. Kele ◽  
Eric J. van der Jagt ◽  
Annette S. H. Gouw ◽  
Ton Lisman ◽  
Robert J. Porte ◽  
...  
Keyword(s):  
Liver Regeneration ◽  
Hepatic Steatosis ◽  
Partial Hepatectomy ◽  
The Impact

scholarly journals Participation of 5-lipoxygenase and LTB4 in liver regeneration after partial hepatectomy

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Florencia Lorenzetti ◽  
Marina Cecilia Vera ◽  
María Paula Ceballos ◽  
María Teresa Ronco ◽  
Gerardo Bruno Pisani ◽  
...  
Keyword(s):  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Time Course ◽  
Specific Inhibitor ◽  
Ph Control ◽  
Initial Time ◽  
Male Wistar Rats ◽  
The Moment ◽  
Parenchymal Cells ◽  
The Impact

AbstractRegeneration is the unmatched liver ability for recovering its functional mass after tissue lost. Leukotrienes (LT) are a family of eicosanoids with the capacity of signaling to promote proliferation. We analyzed the impact of blocking LT synthesis during liver regeneration after partial hepatectomy (PH). Male Wistar rats were subjected to two-third PH and treated with zileuton, a specific inhibitor of 5-lipoxygenase (5-LOX). Our first find was a significant increment of intrahepatic LTB4 during the first hour after PH together with an increase in 5-LOX expression. Zileuton reduced hepatic LTB4 levels at the moment of hepatectomy and also inhibited the increase in hepatic LTB4. This inhibition produced a delay in liver proliferation as seen by decreased PCNA and cyclin D1 nuclear expression 24 h post-PH. Results also showed that hepatic LTB4 diminution by zileuton was associated with a decrease in NF-ĸB activity. Additionally, decreased hepatic LTB4 levels by zileuton affected the recruitment of neutrophils and macrophages. Non-parenchymal cells (NPCs) from zileuton-treated PH-rats displayed higher apoptosis than NPCs from PH control rats. In conclusion, the present work provides evidences that 5-LOX activation and its product LTB4 are involved in the initial signaling events for liver regeneration after PH and the pharmacological inhibition of this enzyme can delay the initial time course of the phenomenon.

scholarly journals Parenteral Nutrition in Liver Resection

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Carlo Chiarla ◽  
Ivo Giovannini ◽  
Felice Giuliante ◽  
Francesco Ardito ◽  
Maria Vellone ◽  
...  
Keyword(s):  
Liver Resection ◽  
Parenteral Nutrition ◽  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Clinical Studies ◽  
Short Review ◽  
Artificial Nutrition ◽  
Postoperative Problems ◽  
The Impact

Albeit a very large number of experiments have assessed the impact of various substrates on liver regeneration after partial hepatectomy, a limited number of clinical studies have evaluated artificial nutrition in liver resection patients. This is a peculiar topic because many patients do not need artificial nutrition, while several patients need it because of malnutrition and/or prolonged inability to feeding caused by complications. The optimal nutritional regimen to support liver regeneration, within other postoperative problems or complications, is not yet exactly defined. This short review addresses relevant aspects and potential developments in the issue of postoperative parenteral nutrition after liver resection.

scholarly journals Lack of Multidrug Resistance-associated Protein 4 Prolongs Partial Hepatectomy-induced Hepatic Steatosis

2020 ◽  
Vol 175 (2) ◽  
pp. 301-311
Author(s):  
Ajay C Donepudi ◽  
Gregory J Smith ◽  
Oladimeji Aladelokun ◽  
Yoojin Lee ◽  
Steven J Toro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Multidrug Resistance ◽  
Liver Injury ◽  
Liver Regeneration ◽  
Hepatic Steatosis ◽  
Partial Hepatectomy ◽  
Knockout Mice ◽  
Tissue Loss ◽  
Hepatocyte Proliferation ◽  
Hepatic Lipid

Abstract Multidrug resistance-associated protein 4 (Mrp4) is an efflux transporter involved in the active transport of several endogenous and exogenous chemicals. Previously, we have shown that hepatic Mrp4 expression increases following acetaminophen overdose. In mice, these increases in Mrp4 expression are observed specifically in hepatocytes undergoing active proliferation. From this, we hypothesized that Mrp4 plays a key role in hepatocyte proliferation and that lack of Mrp4 impedes liver regeneration following liver injury and/or tissue loss. To evaluate the role of Mrp4 in these processes, we employed two-third partial hepatectomy (PH) as an experimental liver regeneration model. In this study, we performed PH-surgery on male wildtype (C57BL/6J) and Mrp4 knockout mice. Plasma and liver tissues were collected at 24, 48, and 72 h postsurgery and evaluated for liver injury and liver regeneration endpoints, and for PH-induced hepatic lipid accumulation. Our results show that lack of Mrp4 did not alter hepatocyte proliferation and liver injury following PH as evaluated by Ki-67 antigen staining and plasma alanine aminotransferase levels. To our surprise, Mrp4 knockout mice exhibited increased hepatic lipid content, in particular, di- and triglyceride levels. Gene expression analysis showed that lack of Mrp4 upregulated hepatic lipin1 and diacylglycerol O-acyltransferase 1 and 2 gene expression, which are involved in the synthesis of di- and triglycerides. Our observations indicate that lack of Mrp4 prolonged PH-induced hepatic steatosis in mice and suggest that Mrp4 may be a novel genetic factor in the development of hepatic steatosis.

The Impact of the Intra-Abdominal Space on Liver Regeneration After a Partial Hepatectomy in Rats

2011 ◽  
Vol 171 (1) ◽  
pp. 259-265 ◽  
Author(s):  
Kensuke Miyazaki ◽  
Susumu Eguchi ◽  
Tetsuo Tomonaga ◽  
Takamitsu Inokuma ◽  
Koji Hamasaki ◽  
...  
Keyword(s):  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
The Impact

scholarly journals Impact of hypertension in liver regeneration in rats

2012 ◽  
Vol 27 (7) ◽  
pp. 460-464 ◽  
Author(s):  
Maria de Lourdes Pessole Biondo-Simões ◽  
Camila Gadens Zamboni ◽  
Evelise Martins ◽  
Luka David Lechinewski ◽  
Sérgio Ossamu Ioshii ◽  
...  
Keyword(s):  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Stellate Cell ◽  
Mass Gain ◽  
Reduction Factor ◽  
Control Group ◽  
Histopathological Analysis ◽  
Liver Mass ◽  
The Difference ◽  
The Impact

PURPOSE: To determine the impact of hypertension in liver regeneration, in rats by examining gain in liver mass and the replication of hepatocytes and stellate cells. METHODS: Forty Wistar rats were allocated into two groups of twenty, the control and experiment group. The experiment group animals were submitted to induction of renovascular hypertension. A week later, all the animals underwent a partial hepatectomy. Measurements were taken after 24 hours and seven days, when ten animals in each group were euthanized. Thus, four subgroups were obtained. The livers were excised and sent for histopathological analysis. RESULTS: The control group had a greater gain in liver mass than the experiment group seven days after partial hepatectomy (p=0.0051). The difference in the activate stellate cell count was not statistically significant following analysis after both 24 hours and seven days (p=1.0). A higher number of dividing hepatocytes was observed in the control group seven days after partial hepatectomy (p=0.0014). CONCLUSION: In rats, hypertension had no direct influence on stellate cell replication, but led to a delay in liver mass gain and were shown to be a reduction factor on hepatocyte replication seven7 days after partial hepatectomy.

The impact of hepatic steatosis on portal hypertension

2018 ◽  
Author(s):  
G Semmler ◽  
B Scheiner ◽  
P Schwabl ◽  
T Bucsics ◽  
R Paternostro ◽  
...  
Keyword(s):  
Portal Hypertension ◽  
Hepatic Steatosis ◽  
The Impact
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