Effects of Bacillus subtilis and its metabolites on corn silage quality

2021 ◽  
Author(s):  
Daiane Silva Bonaldi ◽  
Beatriz Ferreira Carvalho ◽  
Carla Luiza da Silva Ávila ◽  
Cristina Ferreira Silva
Keyword(s):  
Bacillus Subtilis ◽  
Corn Silage ◽  
Silage Quality

scholarly journals Can soybean biomass addition optimize corn silage quality?

2020 ◽  
Vol 15 (3) ◽  
pp. 1-7
Author(s):  
V.V. Batista ◽  
P.F. Adami ◽  
E.S. Lemes ◽  
K.F. Oligini ◽  
C.L. Giacomel ◽  
...  
Keyword(s):  
Corn Silage ◽  
Silage Quality

scholarly journals SILAGE QUALITY OF CORN AND SORGHUM ADDED WITH FORAGE PEANUTS

2016 ◽  
Vol 29 (2) ◽  
pp. 465-472 ◽  
Author(s):  
WALKÍRIA GUIMARÃES CARVALHO ◽  
KÁTIA APARECIDA DE PINHO COSTA ◽  
PATRÍCIA SOARES EPIFANIO ◽  
ROZANA CASTRO PERIM ◽  
DANIEL AUGUSTO ALVES TEIXEIRA ◽  
...  
Keyword(s):  
Zea Mays ◽  
Experimental Design ◽  
Sorghum Bicolor ◽  
Crude Protein ◽  
Corn Silage ◽  
Efficient Technique ◽  
Arachis Pintoi ◽  
Silage Quality ◽  
Sorghum Silage

ABSTRACT: Corn and sorghum are standard silage crops because of their fermentative characteristics. While corn and sorghum silages have lower crude protein (CP) contents than other crops, intercropping with legumes can increase CP content. Furthermore, one way to increase CP content is the addition of legumes to silage. Consequently, the research objective was to evaluate the fermentative and bromatological characteristics of corn (Zea mays) and sorghum (Sorghum bicolor) silages added with forage peanuts (Arachis pintoi). The experimental design was completely randomized with four replicates. The treatments consisted of corn silage, sorghum silage, forage peanut silage, corn silage with 30% forage peanut, and sorghum silage with 30% forage peanut. The results showed that the corn and sorghum added with peanut helped to improve the silage fermentative and bromatological characteristics, proving to be an efficient technique for silage quality. The forage peanut silage had lower fermentative characteristics than the corn and sorghum silages. However, the forage peanut silage had a greater CP content, which increased the protein contents of the corn and sorghum silages when intercropped with forage peanuts.

scholarly journals Changes in the nutritive value and aerobic stability of corn silages inoculated with Bacillus subtilis alone or combined with Lactobacillus plantarum

2016 ◽  
Vol 56 (11) ◽  
pp. 1867 ◽  
Author(s):  
Erika C. Lara ◽  
Fernanda C. Basso ◽  
Flávia B. de Assis ◽  
Fernando A. Souza ◽  
Telma T. Berchielli ◽  
...  
Keyword(s):  
Organic Matter ◽  
Bacillus Subtilis ◽  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Corn Silage ◽  
In Vitro Digestibility ◽  
Aerobic Stability ◽  
Organic Matter Digestibility ◽  
Corn Plants

Chemical composition, fermentation characteristics, in vitro digestibility and aerobic stability were evaluated in corn silage inoculated with microbial additives in two different experiments. Inoculant treatments (untreated, Bacillus subtilis and B. subtilis combined with Lactobacillus plantarum) were applied to fresh forages. Chopped corn plants (2B655 Hx) were ensiled in laboratory silos for periods of 7, 14, 21 and 63 days to evaluate the fermentation parameters. The experimental silos were weighed to determine gas losses. After the ensiling period, the silage was sampled to determine chemical composition and in vitro organic matter digestibility. To evaluate aerobic stability, chopped corn plants (AG‐1051) were ensiled in laboratory silos that were opened after 96 days of ensiling. The silage was placed in different buckets containing data loggers. The silage was sampled after 0, 4, 8 and 12 days of exposure to air to evaluate the microbial populations and pH. The data were analysed as a completely randomised design using a mixed repeated-measures model in the MIXED procedure of SAS. To evaluate each treatment relative to the fermentation times, a regression analysis using the PROC REG procedure of SAS was applied. A significance level of P < 0.05 was used. Inoculation with both strains increased lactic acid concentration, whereas the use of B. subtilis alone or combined with L. plantarum improved in vitro apparent organic matter digestibility. In the B. subtilis and B. subtilis combined with L. plantarum silages, moulds and yeasts decreased, and aerobic stability was improved. Inoculation with B. subtilis alone or combined with L. plantarum improved the nutritional value and aerobic stability of corn silage.

scholarly journals Determination of the Use ofLactobacillus plantarumandPropionibacterium freudenreichiiApplication on Fermentation Profile and Chemical Composition of Corn Silage

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Norafizah Abdul Rahman ◽  
Mohd Ridzwan Abd Halim ◽  
Noraniza Mahawi ◽  
Hazira Hasnudin ◽  
Jameel R. Al-Obaidi ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Bacterial Culture ◽  
Corn Silage ◽  
Propionibacterium Freudenreichii ◽  
Fermentation Profile ◽  
Silage Quality ◽  
Better Than

Corn was inoculated withLactobacillus plantarumandPropionibacterium freudenreichiisubsp.shermaniieither independently or as a mixture at ensiling, in order to determine the effect of bacterial additives on corn silage quality. Grain corn was harvested at 32–37% of dry matter and ensiled in a 4 L laboratory silo. Forage was treated as follows: bacterial types: B0 (without bacteria-control), B1(L. plantarum), B2 (P. freudenreichiisubsp.shermanii), and B3 (combination ofL. plantarumandP. freudenreichiisubsp.shermanii). Each 2 kg of chopped forage was treated with 10 mL of bacterial culture and allowed to ferment for 27 days. The first experiment determined the most suitable wavelength for detection of bacteria (490 nm and 419 nm for B1 and B2, resp.) and the preferable inoculation size (1 × 105 cfu/g). The second experiment analysed the effect of B1 and B2 applied singly or as a mixture on the fermentation characteristics and quality of corn silage.L. plantarumalone increased crude protein (CP) and reduced pH rapidly. In a mixture withP. freudenreichii, the final pH was the lowest compared to other treatments. As a mixture, inclusion of bacteria resulted in silage with lower digestibility than control. Corn silage treated withL. plantarumorP. freudenreichiieither alone or mixed together produced desirable silage properties; however, this was not significantly better than untreated silage.

Evaluation of two biodegradable coatings on corn silage quality

2005 ◽  
Vol 86 (3) ◽  
pp. 392-400 ◽  
Author(s):  
Patrick Denoncourt ◽  
André Amyot ◽  
Monique Lacroix
Keyword(s):  
Corn Silage ◽  
Silage Quality ◽  
Biodegradable Coatings

scholarly journals Effect of additives and filling methods on whole plant corn silage quality, fermentation characteristics and in situ digestibility

2021 ◽  
Author(s):  
Ting Jiao ◽  
Zhaomin Lei ◽  
Jianping Wu ◽  
Fei Li ◽  
David P Casper ◽  
...  
Keyword(s):  
Corn Silage ◽  
Effect Of Additives ◽  
Whole Plant ◽  
Silage Quality

scholarly journals Lentilactobacillus hilgardii Inoculum, Dry Matter Contents at Harvest and Length of Conservation Affect Fermentation Characteristics and Aerobic Stability of Corn Silage

2021 ◽  
Vol 12 ◽  
Author(s):  
Francesco Ferrero ◽  
Ernesto Tabacco ◽  
Giorgio Borreani
Keyword(s):  
Acetic Acid ◽  
Dry Matter ◽  
Corn Silage ◽  
Microbial Count ◽  
Dependent Manner ◽  
Aerobic Stability ◽  
Fermentation Quality ◽  
Silage Quality ◽  
Silage Inoculant ◽  
Positive Effect

Heterofermentative Lentilactobacillus hilgardii isolated from sugarcane silage, has recently been proposed as a silage inoculant to increase aerobic stability. Various conditions can influence the activity of LAB and their ability to alter silage quality (e.g., DM content and length of conservation). The aim of this study has been to evaluate the effect of L. hilgardii on the fermentation quality and aerobic stability of whole crop corn silage with different DM contents (from 26 to 45%), conserved for various conservation lengths (13–272 days). The silages were analyzed for their DM content, pH, fermentative profile, microbial count, and aerobic stability. L. hilgardii showed a positive effect on improving the aerobic stability of silages, due its ability to produce acetic acid, and reduced the yeast count. The acetic acid content increased as the conservation period increased and decreased as the DM content increased. The yeast count was reduced during conservation in a DM dependent manner and the inoculation with LH determined a reduction in the count of 0.48 log cfu/g. The aerobic stability increased as the conservation period increased, and the treatment with LH on average increased the aerobic stability by 19 h. The results of this experiment suggest that higher aerobic stability could be achieved in corn silages by ensiling at medium or low DM contents, or by increasing the length of conservation if a higher DM content at ensiling is needed. The inoculation with LH helps to improve the aerobic stability of corn silages by reducing the yeast count.

scholarly journals Effect of Bacillus amyloliquefaciens and Bacillus subtilis on fermentation, dynamics of bacterial community and their functional shifts of whole-plant corn silage

2022 ◽  
Vol 13 (1) ◽  
Author(s):  
Jie Bai ◽  
Marcia Franco ◽  
Zitong Ding ◽  
Lin Hao ◽  
Wencan Ke ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacterial Community ◽  
Bacillus Amyloliquefaciens ◽  
Corn Silage ◽  
Water Soluble ◽  
Soluble Carbohydrate ◽  
Protein Nitrogen ◽  
Positive Effects ◽  
Whole Plant ◽  
Relative Abundances

Abstract Background Bacillus amyloliquefaciens (BA) and Bacillus subtilis (BS) are usually used as feed supplements directly or bacterial inoculants in biological feeds for animals. However, few research have reported the effects of BA and BS on fermentation characteristics and bacterial community successions of whole-plant corn silage during ensiling. If the BA and BS inoculants have positive effects on silages, then they could not only improve fermentation characteristics, but also deliver BA or BS viable cells to ruminants, which would play its probiotic effect. Therefore, the objectives of this study were to investigate the effects of BA and BS on the fermentation, chemical characteristics, bacterial community and their metabolic pathway of whole-plant corn silage. Results Freshly chopped whole-plant corn was inoculated without or with BA and BS, respectively, and ensiled for 1, 3, 7, 14 and 60 d. Results showed that BA and BS inoculations increased lactic acid concentrations of whole-plant corn silages compared with control, and BA inoculation decreased acetic acid concentrations, whereas BS inoculation decreased fiber contents and increased crude protein (CP) content. Higher water-soluble carbohydrate contents and lower starch contents were observed in BA- and BS-inoculated silages compared with that in control. The decreased CP content and increased non-protein nitrogen content were observed in BA-inoculated silage, which was consistent with the higher amino acid metabolism abundances observed in BA-inoculated silage. In addition, it was noteworthy that BA and BS inoculations increased the metabolism of cofactors and vitamins, and decreased the relative abundances of drug resistance: antimicrobial pathways. We also found that the bacterial metabolism pathways were clearly separated into three clusters based on the ensiling times of whole-plant corn silage in the present study. There were no significant differences in bacterial community compositions among the three groups during ensiling. However, BA and BS inoculations decreased the relative abundances of undesirable bacteria such as Acetobacter and Acinetobacter. Conclusion Our findings suggested that the BS strain was more suitable as silage inoculants than the BA strain in whole-plant corn silage in this study.

Sign in / Sign up

Export Citation Format

Share Document