scholarly journals Isolation and identification of Streptomyces sp. Act4Zk, a good producer of Staurosporine and some derivatives

2020 ◽  
Author(s):  
Z. Khosravi Babadi ◽  
G. Ebrahimipour ◽  
J. Wink ◽  
A. Narmani ◽  
C. Risdian
Keyword(s):  
Isolation And Identification ◽  
Streptomyces Sp ◽  
Good Producer

scholarly journals The result of effective microorganisms isolated from wastewater and soil

2021 ◽  
Vol 32 (1) ◽  
pp. 38-44
Author(s):  
Oyunbileg Natsagdorj ◽  
Indra Nyamjav ◽  
Byambasuren Davaasambuu ◽  
Ariunzaya Jargalsaikhan ◽  
Batsukh Chultem
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Heavy Metal Contamination ◽  
Biologically Active ◽  
Molecular Characteristics ◽  
Isolation And Identification ◽  
Effective Microorganisms ◽  
Streptomyces Sp ◽  
Streptomyces Spp ◽  
Rdna Sequencing ◽  
Effective Microorganism

Increasing urbanization and population concentrations are causing environmental problems such as soil and water pollution. In this study, we were present isolation and identification of Streptomyces sp and Rhodobacteria sp. Those effective microorganisms isolated from the wastewater and soil sample of Anand-Khujirt spring resort located in Khujirt soum, Uvurkhangai province in Mongolia. Based on morphological, cultural, and molecular characteristics (16S rDNA sequencing), those strain were identified as Rhodobacter sphaeroides (99% similarity) and Streptomyces cf.griseus (99% similarity). Rhodobacter sphaeroides could be used to reduce heavy metal contamination from soil. Furthermore, Streptomyces cf.griseus is an effective  microorganism, which produces 32 types of biologically active compounds for pharmaceutical or agricultural purposes. Хаягдал ус, хөрснөөс ашигтай бичил биетний цэвэр өсгөвөр ялгасан судалгааны дүнгээс Хотжилт, хүн амын төвлөрөл нэмэгдэж байгаа нь хөрс, усны бохирдол зэрэг хүрээлэн буй орчны асуудлуудыг үүсгэж байна. Бид энэхүү судалгааны ажлаар Өвөрхангай аймгийн Хужирт сумын нутагт орших “Ананд-Хужирт” рашаан сувилал,  аялал жуулчлалын баазын хаягдал ус, хөрснөөс ялгасан ашигтай бичил биетний нутгийн омгууд болох Streptomyces sp, Rhodobacteria sp-ийн нуклеотидын дарааллыг Genetyx software програмыг ашиглан боловсруулалт хийн АНУ-ын Биотехнологийн Мэдээллийн Үндэсний Төвийн мэдээллийн сангаас BLAST хайлт хийж хамгийн ойролцоох зүйлийг тодорхойлсон. Rhodobacter sphaeroides (99% ижил нуклеотидтэй)-ийг хүнд металлын бохирдлыг бууруулахад өргөн ашигладаг бол Streptomyces cf.griseus (99% ижил нуклеотидтэй) нь 32 төрлийн биологийн идэвхит нэгдлийг нийлэгжүүлэгч ашигтай бичил биетэн юм.    Түлхүүр үг: Streptomyces spp, Rhodobacteria spp, хаягдал ус, хөрс,

scholarly journals Isolation and Identification of Streptomyces spp. from Desert and Savanna Soils in Sudan

2020 ◽  
Vol 17 (23) ◽  
pp. 8749
Author(s):  
Mohamed E. Hamid ◽  
Adil Mahgoub ◽  
Abdulrhman J. O. Babiker ◽  
Hussein A. E. Babiker ◽  
Mohammed A. I. Holie ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Antibiotic Production ◽  
Rrna Gene ◽  
Isolation And Identification ◽  
Streptomyces Sp ◽  
Ecological Zones ◽  
Streptomyces Spp ◽  
Gene Sequence Analysis ◽  
Unique Species

The purpose of this study was to investigate streptomycete populations in desert and savanna ecozones in Sudan and to identify species based on 16S rRNA gene sequences. A total of 49 different Streptomyces phenotypes (22 from sites representing the desert and semi-desert ecozone; 27 representing the savanna ecozone) have been included in the study. The isolates were characterized phenotypically and confirmed using 16S rRNA gene sequence analysis. The two ecozones showed both similarities and uniqueness in the types of isolates. The shared species were in cluster 1 (Streptomyces (S.) werraensis), cluster 2 (Streptomyces sp.), cluster 3 (S. griseomycini-like), and cluster 7 (S. rochei). The desert ecozone revealed unique species in cluster 9 (Streptomyces sp.) and cluster 10 (S. griseomycini). Whereas, the savanna ecozone revealed unique species in cluster 4 (Streptomyces sp.), cluster 5 (S. albogriseolus/ S. griseoincarnatus), cluster 6 (S. djakartensis), and cluster 8 (Streptomyces sp.). Streptomycetes are widely distributed in both desert and the savanna ecozones and many of these require full descriptions. Extending knowledge on Streptomyces communities and their dynamics in different ecological zones and their potential antibiotic production is needed.

scholarly journals ISOLATION AND IDENTIFICATION OF Streptomyces spp. PRODUCING CHITINASE ENZYME FROM SEWAGE MUD

2018 ◽  
Vol 5 (1) ◽  
pp. 99
Author(s):  
Putu Ayu Parwati ◽  
Retno Kawuri ◽  
Ni Luh Watiniasih
Keyword(s):  
Isolation And Identification ◽  
Streptomyces Sp ◽  
Streptomyces Spp

Streptomyces merupakan bakteri dari kelompok Actinomycetes. Genus ini diketahui dapat memproduksi berbagai senyawa aktif diantaranya antibiotik, antiviral, dan enzim. Enzim yang dapat ditemukan pada bakteri dan jamur yaitu salah satunya enzim kitinase. Enzim kitinase yang diproduksi oleh bakteri lebih baik dibandingkan kitinase dari sumber lain karena dengan waktu yang relatif singkat dapat melakukan proses perkembangbiakan. Bakteri kitinolitik merupakan agen pengendalian hayati terhadap jamur patogen maupun serangga hama yang sangat potensial karena aktivitas kitinase yang dimiliki. Penelitian ini bertujuan untuk mengisolasi, mengetahui karakteristik Streptomyces spp. dari lumpur selokan dan uji aktivitas enzim kitinase. Aktivitas enzim kitinase diketahui melalui zona bening yang terbentuk pada media uji. Hasil penelitian diperoleh lima spesies Streptomyces spp. dari  sampel lumpur selokan yang diisolasi dengan karakteristik yang berbeda-beda. Hasil penelitian juga menunjukkan bahwa dari lima spesies Streptomyces spp., dua spesies yaitu Streptomyces sp.3 dan Streptomyces sp.5 mampu memproduksi enzim kitinase, sementara tiga spesies Streptomyces lainnya tidak memproduksi enzim kitinase.  Streptomyces sp.3 memproduksi enzim kitinase dengan terbentuknya zona bening sebesar 1,85±0,07 cm dan pada Streptomyces sp.5 terbentuk zona bening sebesar 0,65 ±0,14 cm . Berdasarkan data tersebut Streptomyces sp.3 dan Streptomyces sp.5 memiliki potensi sebagai bakteri kitinolitik yang memiliki manfaat sebagai agen pengendalian hayati.

Isolation and identification of anticandidal compound from Streptomyces sp. VITPK9

2014 ◽  
Vol 50 (5) ◽  
pp. 492-499 ◽  
Author(s):  
P. Sanjenbam ◽  
J. Vinay Gopal ◽  
K. Kannabiran
Keyword(s):  
Isolation And Identification ◽  
Streptomyces Sp

scholarly journals Isolation and identification of microorganisms for polyurethane degradation

2019 ◽  
pp. 57-66
Author(s):  
Julie Tan ◽  
Takuji Ohwada
Keyword(s):  
Bacillus Pumilus ◽  
Environmental Problem ◽  
Soil Samples ◽  
Base Sequence ◽  
Pseudomonas Sp ◽  
Isolation And Identification ◽  
Streptomyces Sp ◽  
Streptomyces Spp ◽  
Sequence Patterns ◽  
Polyurethane Degradation

Plastic wastes decomposition has been a pressing environmental problem worldwide. In this study, polyurethane (PUR), a thermoset plastic was tested for biodegradation by polyurethane-degrading microorganisms that were isolated from a dumpsite at Kamishihoro, Tokachi, Obihiro, Japan. Actinomycetes were the most abundant microorganisms from soil samples. From the 65 isolated microbial species, 16 possessed polyurethane-degrading ability. These isolates exhibited clearing zones on Yeast-extract salts + Agar and gelatin with polyurethane (YES-AG + PUR). The PUR-degrading isolates were characterized and identified based on their DNA sequence patterns. Some isolates belong to the same genus or species. They were Bacillus chitinolyticus (B03, B04, B07), Streptomyces spp. (B13, B19, C13a, C15, C17a, C17b), Pseudomonas sp. (B20), Bacillus pumilus(B21), Streptomyces cuspidosporus (C10b, C18, C19) and Pseudallesscheria baydii (F04, F07). Streptomyces sp. coded as C13a, with base sequence homology of 99.7% with Streptomyces albogriseolus, was believed to produce the highest amount of both exo- and endo polyurethanases. This was demonstrated by the widest clearing zones when broth and cell-bound supernatants were inoculated into the YES-AG + PUR plate.

scholarly journals Isolation and Identification of Secondary Metabolites FromStreptomycessp. SP301

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986179 ◽  
Author(s):  
Mengyujie Liu ◽  
Peng Shi ◽  
Chunhua Lu ◽  
Lihong Zhong
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Secondary Metabolites ◽  
Fermentation Medium ◽  
Aminobenzoic Acid ◽  
Isolation And Identification ◽  
Streptomyces Sp ◽  
Starter Unit ◽  
New Compounds

Seven compounds, namely aminobenzoates A to D (1-4), naphthalenecarboxylates A and B (5-6), and glycosylatelactone A (7), were isolated from the fermentation medium of Streptomyces sp. SP301. Of these, aminobenzoates C and D (3-4), naphthalenecarboxylate B (6), and glycosylatelactone A (7) are new compounds. Aminobenzoates A to D (1-4) shared a common aromatic starter unit, para-aminobenzoic acid , and biosynthesis involving a different pathway. The structures were elucidated on the basis of 1D- and 2D-Nuclear Magnetic Resonance (NMR) spectroscopy and HR-ESIMS analysis.

scholarly journals Isolation and Identification of Pentalenolactone Analogs from Streptomyces sp. NRRL S-4

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7377
Author(s):  
Huanhuan Li ◽  
Hongji Li ◽  
Shuo Chen ◽  
Wenhui Wu ◽  
Peng Sun
Keyword(s):  
Density Functional ◽  
Genome Mining ◽  
Biosynthetic Gene Cluster ◽  
Antimicrobial Activities ◽  
Gram Negative Bacteria ◽  
Genomic Information ◽  
Isolation And Identification ◽  
Terpene Synthases ◽  
Functional Theory ◽  
Streptomyces Sp

Terpene synthases are widely distributed in Actinobacteria. Genome sequencing of Streptomyces sp. NRRL S-4 uncovered a biosynthetic gene cluster (BGC) that putatively synthesizes pentalenolactone type terpenes. Guided by genomic information, the S-4 strain was chemically investigated, resulting in the isolation of two new sesquiterpenoids, 1-deoxy-8α-hydroxypentalenic acid (1) and 1-deoxy-9β-hydroxy-11-oxopentalenic acid (2), as shunt metabolites of the pentalenolactone (3) biosynthesis pathway. Their structures and absolute configurations were elucidated by analyses of HRESIMS and NMR spectroscopic data as well as time-dependent density functional theory/electronic circular dichroism (TDDFT/ECD) calculations. Compounds 1 and 2 exhibited moderate antimicrobial activities against Gram-positive and Gram-negative bacteria. These results confirmed that the pentalenolactone pathway was functional in this organism and will facilitate efforts for exploring Actinobacteria using further genome mining strategies.

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