Development of quinolone resistance and prevalence of different virulence genes among Shigella flexneri and Shigella dysenteriae in environmental water samples

2020 ◽  
Vol 71 (1) ◽  
pp. 86-93 ◽  
Author(s):  
B. Roy ◽  
S.K. Tousif Ahamed ◽  
B. Bandyopadhyay ◽  
N. Giri
Keyword(s):  
Water Samples ◽  
Virulence Genes ◽  
Shigella Flexneri ◽  
Environmental Water ◽  
Quinolone Resistance ◽  
Shigella Dysenteriae ◽  
Environmental Water Samples

scholarly journals Real-Time PCR Assays for Quantification ofqnrGenes in Environmental Water Samples and Chicken Feces

2012 ◽  
Vol 79 (5) ◽  
pp. 1743-1745 ◽  
Author(s):  
Elisabet Marti ◽  
José Luis Balcázar
Keyword(s):  
Real Time ◽  
Water Samples ◽  
Real Time Pcr ◽  
Environmental Water ◽  
Quinolone Resistance ◽  
Environmental Water Samples ◽  
Complex Samples ◽  
Chicken Feces ◽  
Pcr Assays

ABSTRACTReal-time PCR assays were developed for the enumeration of plasmid-mediated quinolone resistance (PMQR) determinants, such as theqnrA,qnrB, andqnrSgenes, in different water samples and chicken feces. The results indicate that the developed assays are specific and sensitive for the quantification ofqnrgenes in complex samples.

Improving Mycobacterium spp. detection in water: a new methodological proposal

2004 ◽  
Vol 4 (2) ◽  
pp. 103-106
Author(s):  
R. Santos ◽  
S. Gonçalves ◽  
F. Macieira ◽  
F. Oliveira ◽  
R. Rodrigues ◽  
...  
Keyword(s):  
Water Samples ◽  
Recovery Rate ◽  
Opportunistic Infections ◽  
Environmental Water ◽  
Environmental Water Samples ◽  
Sample Treatment ◽  
Detection Time ◽  
Fast Detection ◽  
Enrichment Step ◽  
Mycobacterium Spp

In recent years, non-tuberculous mycobacteria (NTM), once considered merely environmental saprophytes, have emerged as a major cause of opportunistic infections. There is no evidence of human-to-human transmission but they have been found in several environmental water samples. It is, therefore, of the utmost importance to develop methods of rapidly and accurately detecting non-tuberculous mycobacteria in water samples. To obtain a maximum recovery rate and a reduction of Mycobacterium spp. detection time in water samples, different decontamination, enrichment procedures and antibiotics supplements were tested before the inoculation into the Bactec® system. The proposed method of sample treatment (decrease in the decontamination time, followed for a peptone pre-enrichment step and an aztreonam and cefepime supplement) before the inoculation into the Bactec® system proved to be a good option for reliable and fast detection of Mycobacterium spp. in water samples.

The Occurrence of Male-Specific and Somatic Bacteriophages in Polluted South African Waters

1991 ◽  
Vol 24 (2) ◽  
pp. 251-254 ◽  
Author(s):  
R. Kfir ◽  
P. Coubrough ◽  
W. O. K. Grabow
Keyword(s):  
South African ◽  
Water Samples ◽  
Biological Treatment ◽  
Environmental Water ◽  
Environmental Water Samples ◽  
Resistance Pattern ◽  
Male Specific

The occurrence of somatic (F') and male-specific (F') coliphages and Salmonella phages in a variety of environmental water samples was studied using different bacterial hosts. The number of plaque-forming units (pfu) of the different bacteriophages were compared and their resistance pattern to a biological treatment (humus tank) and chlorination was evaluated. The presence of the bacteriophages in shellfish was also studied. The morphology of isolate bacteriophages was examined as well as the visibility of the different plaques formed. Coliphages were found to produce larger and clearer plaques than all other bacteriophages studied. In most of the environmental water samples coliphages outnumbered all other bacteriophages, with the exception of dam water in which higher levels of F' Salmonella phages were detected. The majority of the F' Salmonella phages were shown to be RNA bacteriophages.

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