Differentiation ofClavibacter michiganensissubsp.sepedonicususing PCR melting profile and variable number of tandem repeat methods

2018 ◽  
Vol 68 (1) ◽  
pp. 24-30 ◽  
Author(s):  
A. Żaczek ◽  
K. Struś ◽  
A. Sokołowska ◽  
P. Parniewski ◽  
A. Wojtasik ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Variable Number ◽  
Melting Profile

scholarly journals Multiple-Locus Variable-Number Tandem Repeat Analysis of Dutch Bordetella pertussis Strains Reveals Rapid Genetic Changes with Clonal Expansion during the Late 1990s

2004 ◽  
Vol 186 (16) ◽  
pp. 5496-5505 ◽  
Author(s):  
Leo M. Schouls ◽  
Han G. J. van der Heide ◽  
Luc Vauterin ◽  
Paul Vauterin ◽  
Frits R. Mooi
Keyword(s):  
The Netherlands ◽  
Tandem Repeat ◽  
Bordetella Pertussis ◽  
Minimum Spanning Tree ◽  
Whooping Cough ◽  
Variable Number Tandem Repeat ◽  
Clonal Expansion ◽  
Variable Number ◽  
Multiple Locus ◽  
Repeat Analysis

ABSTRACT Bordetella pertussis, the causative agent of whooping cough, has remained endemic in The Netherlands despite extensive nationwide vaccination since 1953. In the 1990s, several epidemic periods have resulted in many cases of pertussis. We have proposed that strain variation has played a major role in the upsurges of this disease in The Netherlands. Therefore, molecular characterization of strains is important in identifying the causes of pertussis epidemiology. For this reason, we have developed a multiple-locus variable-number tandem repeat analysis (MLVA) typing system for B. pertussis. By combining the MLVA profile with the allelic profile based on multiple-antigen sequence typing, we were able to further differentiate strains. The relationships between the various genotypes were visualized by constructing a minimum spanning tree. MLVA of Dutch strains of B. pertussis revealed that the genotypes of the strains isolated in the prevaccination period were diverse and clearly distinct from the strains isolated in the 1990s. Furthermore, there was a decrease in diversity in the strains from the late 1990s, with a remarkable clonal expansion that coincided with the epidemic periods. Using this genotyping, we have been able to show that B. pertussis is much more dynamic than expected.

scholarly journals Multi-Locus Variable Number of Tandem Repeat Analysis for Rapid and Accurate Typing of Virulent Multidrug Resistant Escherichia coli Clones

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e41232 ◽  
Author(s):  
Umaer Naseer ◽  
Barbro E. Olsson-Liljequist ◽  
Neil Woodford ◽  
Hiran Dhanji ◽  
Rafael Cantón ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Tandem Repeat ◽  
Multidrug Resistant ◽  
Variable Number ◽  
Repeat Analysis
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