scholarly journals MoNSTR-seq, a restriction site-associated DNA sequencing technique to characterize Agrobacterium -mediated transfer-DNA insertions in Phomopsis longicolla

2017 ◽  
Vol 66 (1) ◽  
pp. 19-24 ◽  
Author(s):  
M. Zaccaron ◽  
S. Sharma ◽  
B.H. Bluhm
Keyword(s):  
Dna Sequencing ◽  
Restriction Site ◽  
Phomopsis Longicolla ◽  
Sequencing Technique

IDENTIFYING THE C677T POLYMORPHISM OF MTHFR GENE BY PCR-RFLP TECHNIQUE

2011 ◽  
pp. 28-35
Author(s):  
Keyword(s):  
Internal Control ◽  
Restriction Site ◽  
Mthfr Gene ◽  
C677t Polymorphism ◽  
Mthfr Genotype ◽  
Restriction Digest ◽  
Genotype Frequencies ◽  
Pcr Rflp ◽  
Sequencing Technique ◽  
Volunteer Group

Background: The C677T polymorphism of MTHFR gene is a risk factor of many diseases. This study is aimed at: (1) Improving a PCR-RFLP process with the own designed primers to identify the C677T polymorphism of MTHFR gene. (2) Evaluating the prevalence of the C677T polymorphism of MTHFR gene in volunteer group. Materials and method: DNA samples was extracted from peripheral blood of 60 volunteers. Designing primers by using FastPCR software, then improving PCR technique. Standardizing the optimal conditions of restriction digest by HinfI. Confirming the results of polymorphism by DNA sequencing technique. Results: We designed successfully primers to amplify fragment of MTHFR gene including C677T polymorphism and an obligatory restriction site of HinfI (as internal control). 0.5 µl of HinfI enzyme (10 U/µl) is enough for restriction digest. The MTHFR genotype frequencies were: 71.67 % (677CC); 25% (677CT); and 3.33 % (677TT). Conclusion: We standardized successfully PCR-RFLP technique to identifying C677T polymorphism of MTHFR gene. Keywords: C677T polymorphism, MTHFR gene, PCR-RFLP

scholarly journals Construction of High-Density Linkage Maps of Populus deltoides × P. simonii Using Restriction-Site Associated DNA Sequencing

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0150692 ◽  
Author(s):  
Chunfa Tong ◽  
Huogen Li ◽  
Ying Wang ◽  
Xuran Li ◽  
Jiajia Ou ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Restriction Site ◽  
Populus Deltoides ◽  
High Density ◽  
Linkage Maps

scholarly journals Construction of a highly saturated Genetic Map for Vitis by Next-generation Restriction Site-associated DNA Sequencing

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Junchi Zhu ◽  
Yinshan Guo ◽  
Kai Su ◽  
Zhendong Liu ◽  
Zhihua Ren ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Genetic Map ◽  
Restriction Site ◽  
Next Generation

scholarly journals Restriction-Site-Associated DNA Sequencing Reveals a CrypticViburnumSpecies on the North American Coastal Plain

2018 ◽  
Vol 68 (2) ◽  
pp. 187-203 ◽  
Author(s):  
Elizabeth L Spriggs ◽  
Deren A R Eaton ◽  
Patrick W Sweeney ◽  
Caroline Schlutius ◽  
Erika J Edwards ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Coastal Plain ◽  
North American ◽  
Restriction Site ◽  
The North ◽  
North American Coastal Plain

Novel OTOF gene mutations identified using a massively parallel DNA sequencing technique in DFNB9 deafness

2018 ◽  
Vol 138 (10) ◽  
pp. 865-870 ◽  
Author(s):  
Yanfei Wang ◽  
Yu Lu ◽  
Jing Cheng ◽  
Lei Zhang ◽  
Dongyi Han ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Gene Mutations ◽  
Massively Parallel ◽  
Sequencing Technique ◽  
Massively Parallel Dna Sequencing

scholarly journals SNP discovery of Korean short day onion inbred lines using double digest restriction site-associated DNA sequencing

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0201229 ◽  
Author(s):  
Ji-Hee Lee ◽  
Sathishkumar Natarajan ◽  
Manosh Kumar Biswas ◽  
Kenta Shirasawa ◽  
Sachiko Isobe ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Restriction Site ◽  
Inbred Lines ◽  
Snp Discovery ◽  
Short Day
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