scholarly journals Bacillusspore wet heat resistance and evidence for the role of an expanded osmoregulatory spore cortex

2016 ◽  
Vol 63 (4) ◽  
pp. 247-253 ◽  
Author(s):  
L. Rao ◽  
X. Liao ◽  
P. Setlow
Keyword(s):  
Heat Resistance ◽  
Wet Heat

Resistance Properties and the Role of the Inner Membrane and Coat of Bacillus subtilis Spores with Extreme Wet Heat Resistance

2021 ◽  
Author(s):  
Julia Kanaan ◽  
Jillian Murray ◽  
Ryan Higgins ◽  
Mishil Nana ◽  
Angela M. DeMarco ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Heat Resistance ◽  
Inner Membrane ◽  
Wet Heat

Properties of spores of Bacillus subtilis with or without a transposon that decreases spore germination and increases spore wet heat resistance

2021 ◽  
Author(s):  
Yannong Luo ◽  
George Korza ◽  
Angela M. DeMarco ◽  
Oscar P. Kuipers ◽  
Yong‐qing Li ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Heat Resistance ◽  
Spore Germination ◽  
Wet Heat

scholarly journals Molecular Physiological Characterization of a High Heat Resistant Spore Forming Bacillus subtilis Food Isolate

2021 ◽  
Vol 9 (3) ◽  
pp. 667
Author(s):  
Zhiwei Tu ◽  
Peter Setlow ◽  
Stanley Brul ◽  
Gertjan Kramer
Keyword(s):  
Bacillus Subtilis ◽  
Heat Resistance ◽  
Dipicolinic Acid ◽  
Laboratory Strain ◽  
High Heat ◽  
High Heat Resistance ◽  
Vegetative Cells ◽  
Heat Resistant ◽  
Food Isolate ◽  
Wet Heat

Bacterial endospores (spores) are among the most resistant living forms on earth. Spores of Bacillus subtilis A163 show extremely high resistance to wet heat compared to spores of laboratory strains. In this study, we found that spores of B. subtilis A163 were indeed very wet heat resistant and released dipicolinic acid (DPA) very slowly during heat treatment. We also determined the proteome of vegetative cells and spores of B. subtilis A163 and the differences in these proteomes from those of the laboratory strain PY79, spores of which are much less heat resistant. This proteomic characterization identified 2011 proteins in spores and 1901 proteins in vegetative cells of B. subtilis A163. Surprisingly, spore morphogenic protein SpoVM had no homologs in B. subtilis A163. Comparing protein expression between these two strains uncovered 108 proteins that were differentially present in spores and 93 proteins differentially present in cells. In addition, five of the seven proteins on an operon in strain A163, which is thought to be primarily responsible for this strain’s spores high heat resistance, were also identified. These findings reveal proteomic differences of the two strains exhibiting different resistance to heat and form a basis for further mechanistic analysis of the high heat resistance of B. subtilis A163 spores.

Role of spore coats in the germinative response of Bacillus cereus to adenosine and its analogues

1992 ◽  
Vol 38 (1) ◽  
pp. 38-44 ◽  
Author(s):  
Sonia Senesi ◽  
Giulia Freer ◽  
Giovanna Batoni ◽  
Simona Barnini ◽  
Anna Capaccioli ◽  
...  
Keyword(s):  
Heat Resistance ◽  
Bacillus Cereus ◽  
Dipicolinic Acid ◽  
Germination Rate ◽  
Sodium Dodecyl ◽  
Rapid Evolution ◽  
Key Words Adenosine ◽  
Germination Process ◽  
Specific Activities

Spores of the strain NCIB 8122 of Bacillus cereus have been depleted of coats by treatment with 0.1% sodium dodecyl sulfate – 200 mM 2-mercaptoethanol – 0.5 M NaCl (pH 9.6). The coat-depleted spores did not show any decrease in viability, heat resistance, refractility, dipicolinic acid content, or specific activities of several protoplastic enzymes. The germinative response of the coat-depleted spores to adenosine and several analogues thereof was found qualitatively similar to that obtained with intact spores. However, germination kinetics appeared to be affected by coat removal, since germination rate measured as loss of refractility was eight times slower even at inducer concentrations 10-fold higher than those required to promote optimal germination response of intact spores. Loss of heat resistance, on the other hand, was hardly affected by coat removal. These results suggest that, even though spore coats are not essential for the triggering reaction, they are required for a rapid evolution of the later events in the germination process. Key words: adenosine analogues, germination-triggering reaction, spore coats, coat-depleted spores, Bacillus cereus.

scholarly journals Heat Shock Proteins Do Not Influence Wet Heat Resistance of Bacillus subtilis Spores

2001 ◽  
Vol 183 (2) ◽  
pp. 779-784 ◽  
Author(s):  
Elizabeth Melly ◽  
Peter Setlow
Keyword(s):  
Bacillus Subtilis ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Heat Resistance ◽  
Significant Role ◽  
Vegetative Cell ◽  
Heat Shock Genes ◽  
Wet Heat ◽  
Shock Proteins

ABSTRACT Spores of Bacillus subtilis are significantly more resistant to wet heat than are their vegetative cell counterparts. Analysis of the effects of mutations in and the expression of fusions of a coding gene for a thermostable β-galactosidase to a number of heat shock genes has shown that heat shock proteins play no significant role in the wet heat resistance of B. subtilis spores.

Research Progress of Heat Hv Insulation Resistance of Macromolecular Composite Materials

2011 ◽  
Vol 391-392 ◽  
pp. 332-335
Author(s):  
Yong Peng Yu
Keyword(s):  
Composite Materials ◽  
Heat Resistance ◽  
Flame Retardant ◽  
Epoxy Resin ◽  
High Performance ◽  
Research Progress ◽  
Insulation Resistance ◽  
Excellent Performance ◽  
Wet Heat

Epoxy resin (EP) with excellent performance was widely used as electronic encapsulation materials, but the traditional EP can not meet require of nowadays electronic encapsulation materials in wet-heat resistance, flame retardant, insulation and other performance. So the current research progress of EP with wet-heat resistance and high-performance was summarized in the field of electronic encapsulation.

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