scholarly journals The postantibiotic effect and post-β-lactamase-inhibitor effect of ceftazidime, ceftaroline and aztreonam in combination with avibactam against target Gram-negative bacteria

2016 ◽  
Vol 63 (2) ◽  
pp. 96-102 ◽  
Author(s):  
C.M. Pillar ◽  
A. Stoneburner ◽  
D.L. Shinabarger ◽  
K.M. Krause ◽  
W.W. Nichols
Keyword(s):  
Gram Negative Bacteria ◽  
Postantibiotic Effect ◽  
Gram Negative ◽  
Lactamase Inhibitor

ETX2514 is a broad-spectrum β-lactamase inhibitor for the treatment of drug-resistant Gram-negative bacteria including Acinetobacter baumannii

2017 ◽  
Vol 2 (9) ◽  
Author(s):  
Thomas F. Durand-Réville ◽  
Satenig Guler ◽  
Janelle Comita-Prevoir ◽  
Brendan Chen ◽  
Neil Bifulco ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Broad Spectrum ◽  
Gram Negative Bacteria ◽  
Drug Resistant ◽  
Gram Negative ◽  
Lactamase Inhibitor

scholarly journals Trends and correlation between antibacterial consumption and carbapenem resistance in Gram-negative bacteria in a tertiary hospital in China from 2012 to 2019

2020 ◽  
Author(s):  
Chunhong Liang ◽  
Xueyan Zhang ◽  
Lijuan Zhou ◽  
Guangyi Meng ◽  
Liqiu Zhong ◽  
...  
Keyword(s):  
Southern China ◽  
Pearson Correlation ◽  
Tertiary Care ◽  
Carbapenem Resistance ◽  
Resistance Rate ◽  
Gram Negative Bacteria ◽  
Effective Measure ◽  
Gram Negative ◽  
Defined Daily Doses ◽  
Lactamase Inhibitor

Abstract Background: To investigate the trends and correlation between antibacterial consumption and carbapenem resistance in Gram-negative bacteria from 2012 to 2019 in a tertiary-care teaching hospital in southern China.Methods: This retrospective study included data from hospital-wide inpatients collected between January 2012 and December 2019. Data on antibacterial consumption were expressed as defined daily doses (DDDs)/1000 patient-days. Antibacterials were classified according to the Anatomical Therapeutic Chemical (ATC) classification system. The trends in antimicrobial usage and resistance were analyzed by linear regression, while Pearson correlation analysis was used for testing the correlation.Results: An increasing trend in the annual consumption of tetracyclines, β-lactam/β-lactamase inhibitor (BL/BLI) combinations, and carbapenems was observed (P < 0.05). The carbapenem resistance of Acinetobacter baumannii (A. baumannii) significantly increased (P < 0.05) from 18% in 2012 to 60% in 2019. Moreover, a significant positive correlation was found between the resistance to carbapenems in A. baumannii (β = 0.745, P < 0.05) and Escherichia coli (E. coli)(β = 0.736, P < 0.05) and the consumption of carbapenems, while the resistance rate of A. baumannii to carbapenems was positively correlated with cephalosporin/β-lactamase inhibitor (C/BLI) combinations (β = 0.910, P < 0.01) and tetracyclines usage (β = 0.745, P < 0.05). We also found that the use of quinolones was positively correlated with the resistance rate of Burkholderia cepacia (B. cepacia) to carbapenems (β = 0.717, P < 0.05), and the increasing use of carbapenems (β=-0.869, P < 0.01) and penicillin/β-Lactamase inhibitor (P/BLI) combinations (β=-0.871, P < 0.01) were significantly correlated with reduced resistance of Enterobacter cloacae (E. cloacae) to carbapenems.Conclusion: Our results revealed significant correlations between the consumption of antibiotics and rates of carbapenem resistance in Gram-negative bacteria. Implementing proper management strategies and reducing the unreasonable use of antibacterial drugs may be an effective measure to reduce the spread of carbapenem-resistant Gram-negative bacteria (CRGN), which should be further confirmed by more studies.

scholarly journals Convenient Test for Screening Metallo-β-Lactamase-Producing Gram-Negative Bacteria by Using Thiol Compounds

2000 ◽  
Vol 38 (1) ◽  
pp. 40-43
Author(s):  
Yoshichika Arakawa ◽  
Naohiro Shibata ◽  
Keigo Shibayama ◽  
Hiroshi Kurokawa ◽  
Tetsuya Yagi ◽  
...  
Keyword(s):  
Mercaptoacetic Acid ◽  
Gram Negative Bacteria ◽  
Inhibitory Zone ◽  
Thiol Compounds ◽  
Disk Diffusion Test ◽  
Gram Negative ◽  
Mercaptopropionic Acid ◽  
Growth Inhibitory ◽  
Resistant Strains ◽  
Lactamase Inhibitor

ABSTRACT A simple disk diffusion test was constructed for detection of IMP-1-type metallo-β-lactamase-producing gram-negative bacteria. Two Kirby-Bauer disks containing ceftazidime (CAZ) and a filter disk containing a metallo-β-lactamase inhibitor were used in this test. Several IMP-1 inhibitors such as thiol compounds including 2-mercaptopropionic acid, heavy metal salts, and EDTA were evaluated for this test. Two CAZ disks were placed on a Mueller-Hinton agar plate on which a bacterial suspension was spread according to the method recommended by the National Committee for Clinical Laboratory Standards. The distance between the disks was kept to about 4 to 5 cm, and a filter disk containing a metallo-β-lactamase inhibitor was placed near one of the CAZ disks within a center-to-center distance of 1.0 to 2.5 cm. For IMP-1-producing strains, the growth-inhibitory zone between the two disks expanded, while no evident change in the shape of the growth-inhibitory zone was observed for CAZ-resistant strains producing serine β-lactamases such as AmpC or SHV-12. As a result, 2 to 3 μl of undiluted 2-mercaptopropionic acid or mercaptoacetic acid able to block IMP-1 activity gave the most reproducible and clearest results, and CAZ-resistant strains producing AmpC or extended-spectrum β-lactamases were distinguishable from IMP-1 producers by this test. A similar observation was made with IMP-1-producing clinical isolates such as Serratia marcescens , Klebsiella pneumoniae , Escherichia coli , Enterobacter cloacae , Enterobacter aerogenes , Citrobacter freundii , Proteus vulgaris , Pseudomonas aeruginosa , Pseudomonas putida , Acinetobacter spp., and Alcaligenes xylosoxidans . The specificity and sensitivity of this test were comparable to those of PCR analysis using bla IMP -specific primers. Therefore, this convenient test would be valuable for daily use in clinical laboratories.

Postantibiotic effect of aminoglycosides on Gram-negative bacteria evaluated by a new method

1988 ◽  
Vol 22 (1) ◽  
pp. 23-33 ◽  
Author(s):  
Barbro Isaksson ◽  
Lennart Nilsson ◽  
Rolf Maller ◽  
Lars Sör´n
Keyword(s):  
New Method ◽  
Gram Negative Bacteria ◽  
Postantibiotic Effect ◽  
Gram Negative

scholarly journals Ceftazidime-avibactam: new rules for the game against multidrug-resistant gram-negative bacteria

2018 ◽  
Vol 20 (1) ◽  
pp. 24-34 ◽  
Author(s):  
Roman S. Kozlov ◽  
Olga U. Stetsiouk ◽  
Irina V. Andreeva
Keyword(s):  
Multidrug Resistant ◽  
Gram Negative Bacteria ◽  
Beta Lactamase ◽  
Efficacy And Safety ◽  
Gram Negative ◽  
Rapid Spread ◽  
The World ◽  
Insight Into ◽  
Lactamase Inhibitor ◽  
Real Clinical Practice

The rapid spread of multidrug-resistant Gram-negative bacteria in hospital settings all over the world makes a demand for the new options to overcome antimicrobial resistance. Ceftazidime-avibactam is the first approved antibiotic that contains a new beta-lactamase inhibitor with unique properties. This review provides insight into the spectrum of activity, pharmacological characteristics, data on efficacy and safety of ceftazidime-avibactam obtained from the clinical trials and real clinical practice, as well as prospects for further studies and clinical application of this new antimicrobial agent.

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