scholarly journals Advantage of MALDI ‐ TOF ‐ MS over biochemical‐based phenotyping for microbial identification illustrated on industrial applications

2016 ◽  
Vol 62 (2) ◽  
pp. 130-137 ◽  
Author(s):  
S.K. Urwyler ◽  
J. Glaubitz
Keyword(s):  
Industrial Applications ◽  
Maldi Tof Ms ◽  
Microbial Identification ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Performances and Reliability of Bruker Microflex LT and VITEK MS MALDI-TOF Mass Spectrometry Systems for the Identification of Clinical Microorganisms

2015 ◽  
Vol 2015 ◽  
pp. 1-18 ◽  
Author(s):  
Kivanc Bilecen ◽  
Gorkem Yaman ◽  
Ugur Ciftci ◽  
Yahya Rauf Laleli
Keyword(s):  
Mass Spectrometry ◽  
Clinical Microbiology ◽  
Identification Accuracy ◽  
Clinical Microbiology Laboratory ◽  
Maldi Tof Ms ◽  
Microbial Identification ◽  
Phenotypic Identification ◽  
Maldi Tof ◽  
Vitek Ms ◽  
Tof Ms

In clinical microbiology laboratories, routine microbial identification is mostly performed using culture based methodologies requiring 24 to 72 hours from culturing to identification. Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) technology has been established as a cost effective, reliable, and faster alternative identification platform. In this study, we evaluated the reliability of the two available MALDI-TOF MS systems for their routine clinical level identification accuracy and efficiency in a clinical microbiology laboratory setting. A total of 1,341 routine phenotypically identified clinical bacterial and fungal isolates were selected and simultaneously analyzed using VITEK MS (bioMérieux, France) and Microflex LT (Bruker Diagnostics, Germany) MALDI-TOF MS systems. For any isolate that could not be identified with either of the systems and for any discordant result, 16S rDNA gene or ITS1/ITS2 sequencing was used. VITEK MS and Microflex LT correctly identified 1,303 (97.17%) and 1,298 (96.79%) isolates to the species level, respectively. In 114 (8.50%) isolates initial phenotypic identification was inaccurate. Both systems showed a similar identification efficiency and workflow robustness, and they were twice as more accurate compared to routine phenotypic identification in our sample pool. MALDITOF systems with their accuracy and robustness offer a good identification platform for routine clinical microbiology laboratories.

scholarly journals Assessment of Reproducibility of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Bacterial and Yeast Identification

2015 ◽  
Vol 53 (7) ◽  
pp. 2349-2352 ◽  
Author(s):  
Lars F. Westblade ◽  
Omai B. Garner ◽  
Karen MacDonald ◽  
Constance Bradford ◽  
David H. Pincus ◽  
...  
Keyword(s):  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Microbial Identification ◽  
Desorption Ionization ◽  
Maldi Tof ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry (MS) has revolutionized the identification of clinical bacterial and yeast isolates. However, data describing the reproducibility of MALDI-TOF MS for microbial identification are scarce. In this study, we show that MALDI-TOF MS-based microbial identification is highly reproducible and can tolerate numerous variables, including differences in testing environments, instruments, operators, reagent lots, and sample positioning patterns. Finally, we reveal that samples of bacterial and yeast isolates prepared for MALDI-TOF MS identification can be repeatedly analyzed without compromising organism identification.

Improvement of a rapid direct blood culture microbial identification protocol using MALDI-TOF MS and performance comparison with SepsiTyper kit

2018 ◽  
Vol 155 ◽  
pp. 1-7 ◽  
Author(s):  
Francesca Di Gaudio ◽  
Serena Indelicato ◽  
Sergio Indelicato ◽  
Maria Rita Tricoli ◽  
Giuseppe Stampone ◽  
...  
Keyword(s):  
Blood Culture ◽  
Performance Comparison ◽  
Maldi Tof Ms ◽  
Microbial Identification ◽  
Maldi Tof ◽  
And Performance ◽  
Tof Ms

scholarly journals Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Matthew Sorensen ◽  
Courtney E. Chandler ◽  
Francesca M. Gardner ◽  
Salma Ramadan ◽  
Prasanna D. Khot ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Lipid Extraction ◽  
Fungal Species ◽  
Steel Matrix ◽  
Maldi Tof Ms ◽  
Microbial Identification ◽  
Maldi Tof ◽  
Lipid Biomarker ◽  
Biomarker Analysis ◽  
Tof Ms

AbstractRapid infection diagnosis is critical to improving patient treatment and outcome. Recent studies have shown microbial lipids to be sensitive and selective biomarkers for identifying bacterial and fungal species and antimicrobial resistance. Practical procedures for microbial lipid biomarker analysis will therefore improve patient outcomes and antimicrobial stewardship. However, current lipid extraction methods require significant hands-on time and are thus not suited for direct adoption as a clinical assay for microbial identification. Here, we have developed a method for lipid extraction directly on the surface of stainless-steel matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) plates, termed fast lipid analysis technique or FLAT, which facilitates the identification of bacterial and fungal species using a sub-60-minute workflow. Additionally, our method detects lipid A modifications in Gram-negative bacteria that are associated with antimicrobial resistance, including to colistin.

MALDI-TOF MS: A return to phenotyping in microbial identification?

2011 ◽  
Vol 34 (1) ◽  
pp. 1 ◽  
Author(s):  
Edward R.B. Moore ◽  
Ramon Rosselló-Móra
Keyword(s):  
Maldi Tof Ms ◽  
Microbial Identification ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Conserved mass peaks in MALDI-TOF mass spectra of bacterial species at the genus and species levels

2018 ◽  
Author(s):  
Wenfa Ng
Keyword(s):  
Mass Spectrum ◽  
Mass Spectra ◽  
Mass Peak ◽  
Biological Basis ◽  
Maldi Tof Ms ◽  
Microbial Identification ◽  
Genus Level ◽  
Maldi Tof ◽  
Maldi Tof Mass Spectra ◽  
Tof Ms

Microbes are identified based on their distinguishing characteristics such as gene sequence or metabolic profile. Nucleic acid approaches such as 16S rRNA gene sequencing provide the gold standard method for microbial identification in the contemporary era. However, mass spectrometry-based microbial identification is gaining credence through ease of use, speed, and reliability. Specifically, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used in identifying bacteria, fungus, molds and archaea to the species level with high accuracy. The approach relies on the existence of unique mass spectrum fingerprint for individual microbial species. By comparing the mass spectrum of an unknown microbe with that catalogued in a reference database of known microorganisms, microbes could be identified through mass spectrum fingerprinting. However, the approach lacks fundamental biological basis given the relative difficulty in assigning specific protein to particular mass peak in the profiled mass spectrum, which hampers a deeper understanding of the mass spectrum obtained. This study seeks to examine the existence of conserved mass peaks in MALDI-TOF mass spectra of bacteria at the species and genus levels using open access data from SpectraBank. Results revealed that conserved mass peaks existed for all bacterial species examined. Large number of conserved mass peaks such as that of Escherichia coli and Morganella morganii suggested more closely-related strains of a species though functional annotation of the mass peaks is required to provide a deeper understanding of the mechanisms underlying the conservation of specific proteins. On the other hand, strains of Staphylococcus aureus and Pseudomonas putida had the least number of conserved mass peaks. Presence of conserved mass peaks in many genus provided further evidence that MALDI-TOF MS microbial identification had a biological basis in identification of microbial species to the genus level. In addition, it also highlighted that a subset of proteins could define the taxonomical boundary between the species and genus level. Finally, existence of only one conserved mass peak in Bacillus genus corroborated the difficulty of discriminating Bacillus species based on MALDI-TOF mass spectra. Similarly, no conserved mass peak at the genus level could be found for the Staphylococcus genus. Overall, existence of conserved mass peaks of bacteria at the species and genus levels provided evidence of a firm biological basis in the mass spectrum fingerprinting approach of MALDI-TOF MS microbial identification. This could help identify specific species in mass spectrum of single or multiple microbial species. Further functional annotation of the conserved mass peaks could illuminate in greater detail the biological mysteries of why certain proteins are conserved in specific genus and species.

MALDI-TOF MS for Microbial Identification: Years of Experimental Development to an Established Protocol

2010 ◽  
pp. 255-276 ◽  
Author(s):  
Wibke Kallow ◽  
Marcel Erhard ◽  
Haroun N. Shah ◽  
Emmanuel Raptakis ◽  
Martin Welker
Keyword(s):  
Experimental Development ◽  
Maldi Tof Ms ◽  
Microbial Identification ◽  
Maldi Tof ◽  
Tof Ms
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