scholarly journals Development of a novel multiplex PCR assay for rapid detection of virulence associated genes ofPasteurella multocidafrom pigs

2015 ◽  
Vol 61 (3) ◽  
pp. 293-298 ◽  
Author(s):  
S. Rajkhowa
Keyword(s):  
Multiplex Pcr ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

scholarly journals Multiplex PCR Assay for Rapid Detection and Genotyping of Helicobacter pylori Directly from Biopsy Specimens

2004 ◽  
Vol 42 (6) ◽  
pp. 2821-2824 ◽  
Author(s):  
S. Chattopadhyay ◽  
R. Patra ◽  
T. Ramamurthy ◽  
A. Chowdhury ◽  
A. Santra ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Biopsy Specimens ◽  
Multiplex Pcr Assay

Development of multiplex PCR assay for rapid detection Listeria monocytogenes in clinical samples

2021 ◽  
Vol 30 (4) ◽  
pp. 20-26
Author(s):  
Le Thanh Huong ◽  
Ha Thi Phuong Mai ◽  
Hoang Thi Thu Ha ◽  
Nguyen Dong Tu ◽  
Bui Tien Sy ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Pathogenic Bacteria ◽  
Vulnerable Groups ◽  
Clinical Samples ◽  
Specific Gene ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Specificity And Sensitivity

Listeria monocytogenes is widely present in the natural environment. This bacteria can cause infections in both humans and animals. In humans, the most vulnerable groups to be infected with L. monocytogenes are the elderly, people with an impaired immune system and chronically illness, pregnant women, and newborn babies. The aim of this study was to develop a multiplex PCR assay for the rapid detection of L. monocytogenes in mock clinical samples. A pair of primers were designed for detection of L. monocytogenes based on prs, a Listeria genus specific gene, and hly, a hemolysin gene. The specificity of the primers were tested by using different L. monocytogenes strains and other common pathogenic bacteria. The results showed that L. monocytogenes strains were positive in the detection and other tested strains were negative in mock (spiked) clinical samples. The sensitivity of multiplex PCR assay was 102 CFU/ml per reaction. The specificity and sensitivity of multiplex PCR technology for detecting L. monocytogenes in mock (spiked) clinical samples were high, and the assay could be completed within 1.5 hours. Therefore, this established multiplex PCR provides a rapid and reliable method and will be useful for the detection of L. monocytogenes in mock clinical samples.

Rapid detection of Listeria monocytogenes sequence type 121 strains using a novel multiplex PCR assay

LWT ◽  
2019 ◽  
Vol 116 ◽  
pp. 108474 ◽  
Author(s):  
Moutong Chen ◽  
Jianheng Cheng ◽  
Rui Pang ◽  
Jumei Zhang ◽  
Yuetao Chen ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Sequence Type ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Development of multiplex PCR for rapid detection of metallo-β-lactamase genes in clinical isolates of Acinetobacter baumannii

2020 ◽  
Author(s):  
Reza Ranjbar ◽  
Shahin Zayeri ◽  
Amir Mirzaie
Keyword(s):  
Acinetobacter Baumannii ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Simultaneous Detection ◽  
Cross Reactivity ◽  
Clinical Isolates ◽  
Clinical Samples ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Background and Objectives: Acinetobacter baumannii has been known as a major pathogen causing nosocomial infec- tions. The aim of this study was to develop multiplex PCR for rapid and simultaneous detection of metallo-β-lactamase (MBL) genes in clinical isolates of A. baumannii. Materials and Methods: In this study, we used three sets of primers to amplify the MBL genes including bla        ,     bla   and bla   OXA-48 . The multiplex PCR assay was optimized for rapid and simultaneous detection of MBL genes in A. bau-   OXA-23   NDM   mannii strains recovered from clinical samples. Results: A. baumannii strains recovered from clinical samples were subjected to the study. The multiplex PCR produced 3   OXA-48   OXA-23   bands of 501 bp for bla        , 744 bp for bla observed in multiplex PCR.   OXA-48   and 623 bp for bla   NDM   genes. In addition to, no any cross-reactivity was   Conclusion: Based on obtained data, the multiplex PCR had a good specificity without any cross reactivity and it appears that the multiplex PCR is reliable assay for simultaneous detection of MBL genes in A. baumannii strains.  

scholarly journals Rapid Detection of Methicillin-Resistant Staphylococci from Blood Culture Bottles by Using a Multiplex PCR Assay

2002 ◽  
Vol 40 (8) ◽  
pp. 2786-2790 ◽  
Author(s):  
L. Louie ◽  
J. Goodfellow ◽  
P. Mathieu ◽  
A. Glatt ◽  
M. Louie ◽  
...  
Keyword(s):  
Blood Culture ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Methicillin Resistant ◽  
Multiplex Pcr Assay

scholarly journals Dual Panel Multiplex PCR Assay for Rapid Detection of Medically Important Fungi and Resistant Species of Candida and Aspergillus

2018 ◽  
Vol 47 (3) ◽  
pp. 489-498
Author(s):  
Mohd Hanif Jainlabdin ◽  
Ang Lim Chua ◽  
Tzar Mohd Nizam ◽  
Jacinta Santhanam
Keyword(s):  
Multiplex Pcr ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Resistant Species ◽  
Multiplex Pcr Assay

A novel multiplex PCR assay for rapid detection of virulent Aeromonas in cultured eels

2019 ◽  
Vol 127 (2) ◽  
pp. 418-428 ◽  
Author(s):  
J. Xiong ◽  
B. Huang ◽  
S.-L. Guo ◽  
J.-S. Xu ◽  
W. Huang
Keyword(s):  
Multiplex Pcr ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

scholarly journals Development and application of a multiplex PCR assay for rapid detection of 4 major bacterial pathogens in ducks

2013 ◽  
Vol 92 (5) ◽  
pp. 1164-1170 ◽  
Author(s):  
B. Wei ◽  
S.-Y. Cha ◽  
M. Kang ◽  
I.-J. Park ◽  
O.-K. Moon ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Rapid Detection ◽  
Bacterial Pathogens ◽  
Pcr Assay ◽  
Multiplex Pcr Assay
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