scholarly journals Dual priming oligonucleotide (DPO)-based multiplex PCR assay for specific detection of four diarrhoeagenicEscherichia coliin food

2015 ◽  
Vol 61 (2) ◽  
pp. 146-152 ◽  
Author(s):  
Y.-G. Xu ◽  
Z.-M. Liu ◽  
X.-T. Guan ◽  
L.-C. Cui ◽  
S.-L. Li
Keyword(s):  
Multiplex Pcr ◽  
Specific Detection ◽  
Pcr Assay ◽  
Dual Priming Oligonucleotide ◽  
Multiplex Pcr Assay

scholarly journals Outcome of Colonization of Apis mellifera by Nosema ceranae

2007 ◽  
Vol 73 (20) ◽  
pp. 6331-6338 ◽  
Author(s):  
Raquel Martín-Hernández ◽  
Aránzazu Meana ◽  
Lourdes Prieto ◽  
Amparo Martínez Salvador ◽  
Encarna Garrido-Bailón ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Multiplex Pcr ◽  
Small Subunit ◽  
Nosema Ceranae ◽  
Small Subunit Rrna ◽  
Specific Detection ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Year 2000 ◽  
Single Reaction

ABSTRACT A multiplex PCR-based method, in which two small-subunit rRNA regions are simultaneously amplified in a single reaction, was designed for parallel detection of honeybee microsporidians (Nosema apis and Nosema ceranae). Each of two pairs of primers exclusively amplified the 16S rRNA targeted gene of a specific microsporidian. The multiplex PCR assay was useful for specific detection of the two species of microsporidians related to bee nosemosis, not only in purified spores but also in honeybee homogenates and in naturally infected bees. The multiplex PCR assay was also able to detect coinfections by the two species. Screening of bee samples from Spain, Switzerland, France, and Germany using the PCR technique revealed a greater presence of N. ceranae than of N. apis in Europe, although both species are widely distributed. From the year 2000 onward, statistically significant differences have been found in the proportions of Nosema spp. spore-positive samples collected between and within years. In the first period examined (1999 to 2002), the smallest number of samples diagnosed as Nosema positive was found during the summer months, showing clear seasonality in the diagnosis, which is characteristic of N. apis. From 2003 onward a change in the tendency resulted in an increase in Nosema-positive samples in all months until 2005, when a total absence of seasonality was detected. A significant causative association between the presence of N. ceranae and hive depopulation clearly indicates that the colonization of Apis mellifera by N. ceranae is related to bee losses.

Biosafety evaluation and detection of shrimp viruses on field samples using dual priming oligonucleotide (DPO) system based multiplex PCR assay

Gene Reports ◽  
2021 ◽  
Vol 23 ◽  
pp. 101158
Author(s):  
SiouNing Aileen See ◽  
Zee Hong Goh ◽  
Yen Yew Chan ◽  
Khai En Chong ◽  
Geok Yuan Annie Tan ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Pcr Assay ◽  
Dual Priming Oligonucleotide ◽  
Multiplex Pcr Assay ◽  
Field Samples ◽  
Biosafety Evaluation

Molecular diagnosis of bloodstream infections with a new dual-priming oligonucleotide-based multiplex PCR assay

2013 ◽  
Vol 62 (11) ◽  
pp. 1673-1679 ◽  
Author(s):  
Lucrecia Carrara ◽  
Ferran Navarro ◽  
Miquel Turbau ◽  
Montse Seres ◽  
Indalecio Morán ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Clinical Utility ◽  
Diagnostic Delay ◽  
Bloodstream Infections ◽  
High Specificity ◽  
Pcr Assay ◽  
Dual Priming Oligonucleotide ◽  
Clinical Laboratories ◽  
Multiplex Pcr Assay ◽  
Inappropriate Treatment

Mortality from bloodstream infections (BSIs) correlates with diagnostic delay and the use of inappropriate empirical treatment. Early PCR-based diagnosis could decrease inappropriate treatment, improving patient outcome. The aim of the present study was to assess the clinical utility of this molecular technology to diagnose BSIs. We assessed a new dual-priming oligonucleotide-based multiplex PCR assay, the Magicplex Sepsis Test (MST) (Seegene), along with blood culture (BC). A total of 267 patients from the intensive care unit and haematology and emergency departments were enrolled. Clinical data were also used by physicians to determine the likelihood of infection. Ninety-eight (37 %) specimens were positive: 29 (11 %) by both the MST and BC, 29 (11 %) by the MST only, and 40 (15 %) by BC only. The proportion of agreement between the two methods was 73 % (Cohen’s κ: 0.45; 0.28–0.6; indicating fair to moderate agreement). According to clinical assessment, 63 (64 %) positive specimens were considered BSIs: 23 (36 %) were positive by both the MST and BC, 22 (35 %) were positive only by BC, and 18 (29 %) were positive only by the MST. Thirty-eight (14 %) positive specimens by the MST and/or BC were considered as contaminants. Of 101 specimens collected from patients receiving antibiotics, 20 (20 %) were positive by the MST and 32 (32 %) by BC. Sensitivity and specificity were 65 % and 92 %, respectively, for the MST and 71 % and 88 %, respectively for BC. We concluded that the MST shows a high specificity but changes in design are needed to increase bacteraemia detection. For viability in clinical laboratories, technical improvements are also required to further automate the process.

scholarly journals A multiplex PCR assay for the detection of five influenza viruses using a dual priming oligonucleotide system

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Xuezheng Ma ◽  
Huanzhou Xu ◽  
Lei Shi ◽  
Pengfei Yang ◽  
Liping Zhang ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Influenza Viruses ◽  
Pcr Assay ◽  
Dual Priming Oligonucleotide ◽  
Multiplex Pcr Assay
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