Validation of IRS PCR, a molecular typing method, for the study of the diversity and population dynamics ofLegionellain industrial cooling circuits

2012 ◽  
Vol 56 (2) ◽  
pp. 135-141 ◽  
Author(s):  
D. Jakubek ◽  
M. Le Brun ◽  
G. Leblon ◽  
M. DuBow ◽  
M. Binet
Keyword(s):  
Population Dynamics ◽  
Molecular Typing ◽  
Typing Method ◽  
Molecular Typing Method

scholarly journals Detection of Unsuspected Cases of Nosocomial Transmission of Tuberculosis by Use of a Molecular Typing Method

2001 ◽  
Vol 33 (4) ◽  
pp. 453-459 ◽  
Author(s):  
Griselda Tudó ◽  
Julián González ◽  
Josep M. Gatell ◽  
Joan A. Caylà ◽  
Esteban Martínez ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Nosocomial Transmission ◽  
Typing Method ◽  
Molecular Typing Method

Development of a Molecular Typing Method for Pneumocystis carinii sp.f. hominis

1996 ◽  
Vol 43 (5) ◽  
pp. 34S-34S ◽  
Author(s):  
PHILIPPE M. HAUSER ◽  
DOMINIQUE S. BLANC ◽  
JACQUES BILLE ◽  
AMALIO TELENTI ◽  
PATRICK FRANCIOLI
Keyword(s):  
Molecular Typing ◽  
Pneumocystis Carinii ◽  
Typing Method ◽  
Molecular Typing Method

scholarly journals New Short Tandem Repeat-Based Molecular Typing Method for Pneumocystis jirovecii Reveals Intrahospital Transmission between Patients from Different Wards

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0125763 ◽  
Author(s):  
Maud Gits-Muselli ◽  
Marie-Noelle Peraldi ◽  
Nathalie de Castro ◽  
Véronique Delcey ◽  
Jean Menotti ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Molecular Typing ◽  
Pneumocystis Jirovecii ◽  
Typing Method ◽  
Molecular Typing Method ◽  
Short Tandem

scholarly journals Molecular strategy for ‘serotyping’ of human enteroviruses

2001 ◽  
Vol 82 (1) ◽  
pp. 79-91 ◽  
Author(s):  
Valérie Caro ◽  
Sophie Guillot ◽  
Francis Delpeyroux ◽  
Radu Crainic
Keyword(s):  
Molecular Typing ◽  
Phylogenetic Trees ◽  
Field Isolate ◽  
Human Enterovirus ◽  
Specific Information ◽  
Genomic Fragment ◽  
Coding Region ◽  
Typing Method ◽  
Genome Variability ◽  
Molecular Typing Method

To explore further the phylogenetic relationships between human enteroviruses and to develop new diagnostic approaches, we designed a pair of generic primers in order to study a 1452 bp genomic fragment (relative to the poliovirus Mahoney genome), including the 3′ end of the VP1-coding region, the 2A- and 2B-coding regions, and the 5′ moiety of the 2C-coding region. Fifty-nine of the 64 prototype strains and 45 field isolates of various origins, involving 21 serotypes and 6 strains untypeable by standard immunological techniques, were successfully amplified with these primers. By determining the nucleotide sequence of the genomic fragment encoding the C-terminal third of the VP1 capsid protein we developed a molecular typing method based on RT–PCR and sequencing. If field isolate sequences were compared to human enterovirus VP1 sequences available in databases, nucleotide identity score was, in each case, highest with the homotypic prototype (74.8 to 89.4%). Phylogenetic trees were generated from alignments of partial VP1 sequences with several phylogeny algorithms. In all cases, the new classification of enteroviruses into five identified species was confirmed and strains of the same serotype were always monophyletic. Analysis of the results confirmed that the 3′ third of the VP1-coding sequence contains serotype-specific information and can be used as the basis of an effective and rapid molecular typing method. Furthermore, the amplification of such a long genomic fragment, including non-structural regions, is straightforward and could be used to investigate genome variability and to identify recombination breakpoints or specific attributes of pathogenicity.

scholarly journals Analysis of methicillin-resistant and methicillin-susceptibleStaphylococcus aureusby a molecular typing method based on coagulase gene polymorphisms

1995 ◽  
Vol 115 (3) ◽  
pp. 419-426 ◽  
Author(s):  
N. Kobayashi ◽  
K. Taniguchi ◽  
K. Kojima ◽  
S. Urasawa ◽  
N. Uehara ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Gene Polymorphisms ◽  
Fragment Length Polymorphism ◽  
Restriction Enzyme Digestion ◽  
Rflp Pattern ◽  
Typing Method ◽  
Methicillin Resistant ◽  
Serological Typing ◽  
Molecular Typing Method ◽  
Rflp Typing

SummaryA molecular typing method forStaphylococcus aureusbased on coagulase gene polymorphisms (coagulase gene typing) was evaluated by examining a total of 240 isolates which comprised 210 methicillin-resistantS. aureus(MRSA) and 30 methicillin-susceptibleS. aureus(MSSA) collected from a single hospital. ByAlulrestriction enzyme digestion of the PCR-amplified 3′-end region of the coagulase gene including 81-bp repeated units, the MRSA and MSSA isolates examined were divided into 6 and 12 restriction fragment length polymorphism (RFLP) patterns, respectively, whereas five patterns were commonly detected in MRSA and MSSA. MRSA isolates that showed a particular RFLP pattern were considered to be predominant in the hospital. Coagulase typing with type-specific antisera was also performed for allS. aureusisolates for comparison. Coagulase types II and VII were most frequently detected and included isolates with four and five differentAluIRFLP patterns, respectively, whereas each of the other coagulase types corresponded to a single RFLP pattern. These results indicated that RFLP typing was more discriminatory than serological typing, for typingS. aureusand demonstrated its utility in epidemiologic investigation ofS. aureusinfection in hospitals.

Development of a molecular typing method for Pneumocystis carinii sp.f. hominis

Apmis ◽  
1997 ◽  
Vol 105 (S77) ◽  
pp. 7-10 ◽  
Author(s):  
PHILIPPE M. HAUSER ◽  
DOMINIQUE S. BLANC ◽  
JACQUES BILLE ◽  
AMALIO TELENTI ◽  
PATRICK FRANCIOLI
Keyword(s):  
Molecular Typing ◽  
Pneumocystis Carinii ◽  
Typing Method ◽  
Molecular Typing Method

PCR detection of aminoglycoside resistance genes: a rapid molecular typing method for Acinetobacter baumannii

1999 ◽  
Vol 150 (5) ◽  
pp. 317-322 ◽  
Author(s):  
Isabelle Noppe-Leclercq ◽  
Frédéric Wallet ◽  
Stephanie Haentjens ◽  
René Courcol ◽  
Michel Simonet
Keyword(s):  
Acinetobacter Baumannii ◽  
Resistance Genes ◽  
Molecular Typing ◽  
Pcr Detection ◽  
Aminoglycoside Resistance ◽  
Typing Method ◽  
Molecular Typing Method ◽  
Aminoglycoside Resistance Genes

scholarly journals Simplification of a Locus-Specific DNA Typing Method (Vir Typing) for Streptococcus pyogenes

1998 ◽  
Vol 36 (5) ◽  
pp. 1428-1429 ◽  
Author(s):  
J. Hartas ◽  
M. Hibble ◽  
K. S. Sriprakash
Keyword(s):  
Primary Culture ◽  
Streptococcus Pyogenes ◽  
Molecular Typing ◽  
Dna Typing ◽  
Typing Method ◽  
Molecular Typing Method

We describe a simplification of a highly discriminatory molecular typing method, called Vir typing, for Streptococcus pyogenes (D. Gardiner, J. Hartas, B. Currie, J. D. Mathews, D. J. Kemp, and K. S. Sriprakash, PCR Methods Appl. 4:288–293, 1995). The procedure can be completed within a day, is reproducible, and can be applied directly to colonies growing on primary culture plates, allowing rapid establishment of strain identity in an outbreak.

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