Chromosomal analysis of eight species of dragonflies (Anisoptera) and damselflies (Zygoptera) using conventional cytogenetics and fluorescence in situ hybridization: Insights into the karyotype evolution of the ancient insect order Odonata

2020 ◽  
Author(s):  
Valentina Kuznetsova ◽  
Anna Maryańska‐Nadachowska ◽  
Boris Anokhin ◽  
Nazar Shapoval ◽  
Anatoly Shapoval
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Karyotype Evolution ◽  
Chromosomal Analysis ◽  
Insect Order ◽  
Conventional Cytogenetics

The usefulness of interphase fluorescence in situ hybridization at diagnosis of myeloma in addition to metaphase cytogenetics

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e19558-e19558
Author(s):  
S. Park ◽  
C. Kim ◽  
H. Kim ◽  
D. Hong ◽  
S. Lee ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Plasma Cells ◽  
Chromosomal Abnormalities ◽  
Chromosome Abnormalities ◽  
Interphase Fish ◽  
Conventional Cytogenetics ◽  
Igh Rearrangement

e19558 Background: Multiple myeloma is characterized by the accumulation of malignant plasma cells within the bone marrow and regarded as incurable, but remissions may be induced with steroids, chemotherapy, thalidomide and stem cell transplants. The clinical heterogeneity of myeloma is dictated by the cytogenetic aberrations present in the clonal plasma cells. Fluorescence in situ hybridization (FISH) overcomes the limitations of standard cytogenetics and allows for the detection of numerical and structural chromosomal abnormalities in both metaphase spreads and interphase nuclei. Methods: We evaluated the chromosome abnormalities in 34 MM patients using conventional cytogenetics and interphase FISH with 6 probes such as IGH/CCND1, IGH/FGFR3, IGH/MAF, DS13S319/LAMP1, IGH/BAP, and p53/CEP17. Results: Cytogenetic abnormalities were found in 24 (70.6%) of the 28 MM patients. 10 (35.7%) patients had abnormal metaphases by conventional cytogenetics. Interphase FISH results were abnormal in 21 (61.8%) patients and 11 (52.3%) patients had abnormal interphase FISH but normal metaphases. The evidence of the loss of D13S319 with or without loss of LAMP1 was found in 6 (21.4%) patients, and loss of p53±CEP17 for 2 patients, IGH-BAP for 9 (26.5%) patients, IGH/FGFR3 for 2 patients, and IGH/CCND1 for 7 (20.6%) patients, respectively. However, there were none positive for IGH/MAF. Chromosome 13 abnormalities and IGH rearrangement is correlated with poor clinical outcome. Conclusions: Interphase FISH can provide useful information to evaluate the presence of prognostic chromosome abnormalities in addition to metaphase cytogenetics. And it should be used in the routine evaluation of multiple myeloma. No significant financial relationships to disclose.

Refining the Breakpoints of Three New Translocations Identified in Myelodysplastic Syndromes

2015 ◽  
Vol 135 (2) ◽  
pp. 94-100
Author(s):  
Dolors Costa ◽  
Concha Muñoz ◽  
Ana Carrió ◽  
Amparo Arias ◽  
Cándida Gómez ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Myelodysplastic Syndromes ◽  
Tp53 Gene ◽  
Neoplastic Process ◽  
Conventional Cytogenetics

Recurrent translocations are uncommon in myelodysplastic syndromes (MDS). Three new recurrent translocations, namely der(12)t(3;12)(q13;p13), t(11;13;22)(q13;q14;q12) and der(17)t(13;17)(q21;p13), identified by conventional cytogenetics (CC) in 4 MDS patients, were further characterized using a panel of commercial and homemade fluorescence in situ hybridization (FISH) probes. The goal of this study was to determine the precise breakpoints and to identify genes that could be related with the neoplastic process. Half of the breakpoints (4/8) were precisely identified and in the remaining half they were narrowed to a region ranging from 14 to 926 kb. All the studied breakpoints had interstitial or terminal deletions ranging from 536 kb to 89 Mb, and only those 7 Mb were detected by CC. The genes located in or around the breakpoints described in our study have not been previously related to MDS. The deleted regions include the ETV6 and RB1 genes, among others, and exclude the TP53 gene. FISH studies were useful to refine the breakpoints of the translocations, but further studies are needed to determine the role of the involved genes in the neoplastic process.

Practical Detection of t(14;18)(IgH/BCL2) in Follicular Lymphoma

2008 ◽  
Vol 132 (8) ◽  
pp. 1355-1361
Author(s):  
Keni Gu ◽  
Wing C. Chan ◽  
Robert C. Hawley
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Follicular Lymphoma ◽  
Fluorescence In Situ Hybridization ◽  
Residual Disease ◽  
Response To Therapy ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Conventional Cytogenetics

Abstract The t(14;18)(q32;q21) translocation is the genetic hallmark of follicular lymphoma. Detection of this translocation can facilitate the diagnosis of follicular lymphoma and can be used to monitor response to therapy and level of residual disease. We herein review and compare practical techniques for detecting t(14;18)(q32;q21), including conventional cytogenetics, fluorescence in situ hybridization, Southern blot analysis, and polymerase chain reaction–based assay. Emphasis is placed on fluorescence in situ hybridization and polymerase chain reaction–based assay, given the applicability of these techniques to fixed, paraffin-embedded tissue.

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