scholarly journals Validation of Edwardsiella ictaluri oral vaccination platform in experimental pond trials

2019 ◽  
Vol 51 (2) ◽  
pp. 346-363 ◽  
Author(s):  
David J. Wise ◽  
Terrence E. Greenway ◽  
Todd S. Byars ◽  
Ganesh Kumar ◽  
Matt J. Griffin ◽  
...  
Keyword(s):  
Edwardsiella Ictaluri ◽  
Oral Vaccination ◽  
Experimental Pond

Density-dependent effect on oral vaccination of channel catfish against Edwardsiella ictaluri

Aquaculture ◽  
1994 ◽  
Vol 122 (2-3) ◽  
pp. 91-96 ◽  
Author(s):  
J.A. Plumb ◽  
S. Vinitnantharat ◽  
V. Abe ◽  
R.P. Phelps
Keyword(s):  
Channel Catfish ◽  
Edwardsiella Ictaluri ◽  
Oral Vaccination ◽  
Dependent Effect ◽  
Density Dependent

Methylated N-(4-N,N-dimethylaminocinnamyl) chitosan-coated electrospray OVA-loaded microparticles for oral vaccination

2013 ◽  
Vol 448 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Tittaya Suksamran ◽  
Tanasait Ngawhirunpat ◽  
Theerasak Rojanarata ◽  
Warayuth Sajomsang ◽  
Tasana Pitaksuteepong ◽  
...  
Keyword(s):  
Oral Vaccination

scholarly journals Oral Vaccination of Grass Carp (Ctenopharyngodon idella) with Baculovirus-Expressed Grass Carp Reovirus (GCRV) Proteins Induces Protective Immunity against GCRV Infection

Vaccines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 41
Author(s):  
Changyong Mu ◽  
Qiwang Zhong ◽  
Yan Meng ◽  
Yong Zhou ◽  
Nan Jiang ◽  
...  
Keyword(s):  
Survival Rate ◽  
Grass Carp ◽  
Oral Vaccine ◽  
Serum Antibody ◽  
Ctenopharyngodon Idella ◽  
Control Group ◽  
Oral Vaccination ◽  
Grass Carp Reovirus ◽  
Silkworm Pupae ◽  
Grass Carp Ctenopharyngodon Idella

The grass carp reovirus (GCRV) causes severe hemorrhagic disease with high mortality and leads to serious economic losses in the grass carp (Ctenopharyngodon idella) industry in China. Oral vaccine has been proven to be an effective method to provide protection against fish viruses. In this study, a recombinant baculovirus BmNPV-VP35-VP4 was generated to express VP35 and VP4 proteins from GCRV type Ⅱ via Bac-to-Bac baculovirus expression system. The expression of recombinant VP35-VP4 protein (rVP35-VP4) in Bombyx mori embryo cells (BmE) and silkworm pupae was confirmed by Western blotting and immunofluorescence assay (IFA) after infection with BmNPV-VP35-VP4. To vaccinate the grass carp by oral route, the silkworm pupae expressing the rVP35-VP4 proteins were converted into a powder after freeze-drying, added to artificial feed at 5% and fed to grass carp (18 ± 1.5 g) for six weeks, and the immune response and protective efficacy in grass carp after oral vaccination trial was thoroughly investigated. This included blood cell counting and classification, serum antibody titer detection, immune-related gene expression and the relative percent survival rate in immunized grass carp. The results of blood cell counts show that the number of white blood cells in the peripheral blood of immunized grass carp increased significantly from 14 to 28 days post-immunization (dpi). The differential leukocyte count of neutrophils and monocytes were significantly higher than those in the control group at 14 dpi. Additionally, the number of lymphocytes increased significantly and reached a peak at 28 dpi. The serum antibody levels were significantly increased at Day 14 and continued until 42 days post-vaccination. The mRNA expression levels of immune-related genes (IFN-1, TLR22, IL-1β, MHC I, Mx and IgM) were significantly upregulated in liver, spleen, kidney and hindgut after immunization. Four weeks post-immunization, fish were challenged with virulent GCRV by intraperitoneal injection. The results of this challenge study show that orally immunized group exhibited a survival rate of 60% and relative percent survival (RPS) of 56%, whereas the control group had a survival rate of 13% and RPS of 4%. Taken together, our results demonstrate that the silkworm pupae powder containing baculovirus-expressed VP35-VP4 proteins could induce both non-specific and specific immune responses and protect grass carp against GCRV infection, suggesting it could be used as an oral vaccine.

237 Stable Performance with Reduced Antibiotic Use in Piglets Vaccinated with an E. coli F4/F18 Vaccine for the Prevention of F18-ETEC Post-weaning Diarrhea

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 139-140
Author(s):  
Frédéric A Vangroenweghe
Keyword(s):  
Antibiotic Use ◽  
Watery Diarrhea ◽  
Economic Losses ◽  
Standard Group ◽  
Performance Parameters ◽  
Oral Vaccination ◽  
E Coli ◽  
Technical Performance ◽  
Stable Performance ◽  
The Impact

Abstract Post-weaning Escherichia coli diarrhea (PWD) remains a major cause of economic losses for the pig industry. PWD, caused by enterotoxigenic E. coli (ETEC), typically provokes mild to severe watery diarrhea between 5–10 days after weaning. Recently, an oral live bivalent E. coli F4/F18 vaccine (Coliprotec® F4/F18; Elanco) was approved on the European market, which reduces the impact of PWD provoked by F4-ETEC and F18-ETEC. The objective was to compare technical results and antibiotic use following E. coli F4/F18 vaccination with previous standard therapeutic approach under field conditions. A 1600-sow farm (weaning at 26 days) with diagnosed problems of PWD due to F18-ETEC was selected. Piglets were vaccinated at 21 days with the oral live bivalent E. coli F4/F18 vaccine. At weaning, no standard group medication (ZnO and antibiotics) was applied for prevention of PWD. Several performance parameters were collected: treatment incidence (TI100), mortality and days in nursery. Statistical analysis was performed using JMP 14.0 – comparison of means. Oral E. coli F4/F18 vaccination significantly reduced TI100 (7 ± 2 days to 0 ± 1 days; P < 0.05). Mortality rate remained stable (2.05% in Control to 1.96% in Vaccinated group; P < 0.05). Days in nursery (40 ± 3 days) remained at the same level compared to pre-vaccination. The results show that live E. coli F4/F18 vaccination against PWD has led to similar technical performance parameters and mortality, in combination with a significant reduction in medication use. In conclusion, control of PWD through oral vaccination is a successful option in order to prevent piglets from the negative clinical outcomes of F18-ETEC infection during the post-weaning period.

scholarly journals Maternal transfer of neutralizing antibodies to B. burgdorferi OspA after oral vaccination of the rodent reservoir

Vaccine ◽  
2021 ◽  
Author(s):  
Kathryn Phillip ◽  
Nisha Nair ◽  
Kamalika Samanta ◽  
Jose F. Azevedo ◽  
Grant D. Brown ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Maternal Transfer ◽  
Oral Vaccination

scholarly journals In Vivo Safety Studies With SPBN GASGAS in the Frame of Oral Vaccination of Foxes and Raccoon Dogs Against Rabies

2018 ◽  
Vol 5 ◽  
Author(s):  
Steffen Ortmann ◽  
Antje Kretzschmar ◽  
Christiane Kaiser ◽  
Thomas Lindner ◽  
Conrad Freuling ◽  
...  
Keyword(s):  
Oral Vaccination ◽  
Safety Studies ◽  
Raccoon Dogs

scholarly journals IncA/C Plasmid-Mediated Florfenicol Resistance in the Catfish Pathogen Edwardsiella ictaluri

2008 ◽  
Vol 53 (2) ◽  
pp. 845-846 ◽  
Author(s):  
Timothy J. Welch ◽  
David G. White ◽  
Matt Griffin ◽  
Jason Evenhuis ◽  
Patrick F. McDermott ◽  
...  
Keyword(s):  
Edwardsiella Ictaluri
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