Dietary probiotic Pediococcus acidilactici MA18/5M modulates the intestinal microbiota and stimulates intestinal immunity in rainbow trout ( Oncorhynchus mykiss )

2019 ◽  
Vol 50 (6) ◽  
pp. 1133-1151 ◽  
Author(s):  
Ali Al‐Hisnawi ◽  
Ana Rodiles ◽  
Mark D. Rawling ◽  
Mathieu Castex ◽  
Paul Waines ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Intestinal Microbiota ◽  
Pediococcus Acidilactici ◽  
Intestinal Immunity ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals The Effects of Dietary Insect Meal from Hermetia illucens Prepupae on Autochthonous Gut Microbiota of Rainbow Trout (Oncorhynchus mykiss)

Animals ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 143 ◽  
Author(s):  
Simona Rimoldi ◽  
Elisabetta Gini ◽  
Federica Iannini ◽  
Laura Gasco ◽  
Genciana Terova
Keyword(s):  
Rainbow Trout ◽  
Gut Microbiota ◽  
Oncorhynchus Mykiss ◽  
Intestinal Microbiota ◽  
Control Diet ◽  
Illumina Miseq ◽  
Rrna Gene ◽  
Hermetia Illucens ◽  
Operational Taxonomic Units ◽  
Rainbow Trout Oncorhynchus Mykiss

This study evaluated the effects of dietary insect meal from Hermetia illucens larvae on autochthonous gut microbiota of rainbow trout (Oncorhynchus mykiss). Three diets, with increasing levels of insect meal inclusion (10%, 20%, and 30%) and a control diet without insect meal were tested in a 12-week feeding trial. To analyze the resident intestinal microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME pipeline were used. The number of reads taxonomically classified according to the Greengenes database was 1,514,155. Seventy-four Operational Taxonomic Units (OTUs) at 97% identity were identified. The core of adhered intestinal microbiota, i.e., OTUs present in at least 80% of mucosal samples and shared regardless of the diet, was constituted by three OTUs assigned to Propiobacterinae, Shewanella, and Mycoplasma genera, respectively. Fish fed the insect-based diets showed higher bacterial diversity with a reduction in Proteobacteria in comparison to fish fed the fishmeal diet. Insect-meal inclusion in the diet increased the gut abundance of Mycoplasma, which was attributed the ability to produce lactic and acetic acid as final products of its fermentation. We believe that the observed variations on the autochthonous intestinal microbiota composition of trout are principally due to the prebiotic properties of fermentable chitin.

Sign in / Sign up

Export Citation Format

Share Document