Comparative Characterization of Sequence Structure, Gene Expression, and Enzyme Activity of Alpha-2-macroglobulin betweenStichopus monotuberculatus(Stichopodidae) andHolothuria atra(Holothuriidae)

2016 ◽  
Vol 48 (2) ◽  
pp. 360-373 ◽  
Author(s):  
Jing Qian ◽  
Chunhua Ren ◽  
Jianjun Xia ◽  
Zonghe Yu ◽  
Chaoqun Hu
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Sequence Structure ◽  
Comparative Characterization

scholarly journals ISOLASI KARAKTERISASI DAN UJI EKSPRESI GEN ALP1 DI ESCHERICHIA COLI DH5A

1999 ◽  
Vol 4 (2) ◽  
pp. 61-67
Author(s):  
Ni Nyoman Tri Puspaningsih ◽  
Akhmaloka Akhmaloka ◽  
Sofyan Hadi ◽  
Y. Sri Wulan Manuhara ◽  
Bambang Irawan
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Enzyme Activity ◽  
Nucleotide Sequence ◽  
Recombinant Dna ◽  
Restriction Enzymes ◽  
Cloning Vector ◽  
Amylase Gene

Amylase gene (ALP1) insertion in YCp 50 cloning vector was detected by Direct Screening PCR. Result of Direct Screening PCR showed one transformants which assumed have amylase gene insert. Characterization of the recombinant DNA by Stu1 and EcoR1 restriction enzymes indicated nucleotide sequence of insert gene. Digestion by BamHI, Cla1, and EcoRV restriction enzymes showed only one band that assumed size of insert gene is about 1500 bp. Gene expression showed that amylase enzyme activity by using Somogyi-Nelson method was 88.0265 Unit. This activity was 10 percent higher than transformant control (Escherichia coli DH5a which content YCp50).

Characterization of THLE-Cytochrome P450 (P450) Cell Lines: Gene Expression Background and Relationship to P450-Enzyme Activity

2012 ◽  
Vol 40 (11) ◽  
pp. 2054-2058 ◽  
Author(s):  
Yalda Soltanpour ◽  
Constanze Hilgendorf ◽  
Marie M. Ahlström ◽  
Alison J. Foster ◽  
J. Gerry Kenna ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Cytochrome P450 ◽  
Cell Lines ◽  
P450 Enzyme

A Comprehensive Comparative Characterization of Keratinocytes; The Significant Wound Healing Agents

2016 ◽  
Vol 2 (2) ◽  
pp. 3
Author(s):  
Muhammad Irfan-maqsood ◽  
Hojjat Naderi-Meshkin ◽  
Asieh Heirani-Tabasi ◽  
Monireh Bahrami ◽  
Mahdi Mirahmadi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Comparative Characterization

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

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