Application of Computer-assisted Sperm Analysis in Selecting the Suitable Solution for Common Carp,Cyprinus carpioL., Sperm Motility

2013 ◽  
Vol 44 (3) ◽  
pp. 466-472 ◽  
Author(s):  
Beata Irena Cejko ◽  
Beata Sarosiek ◽  
Radosław Kajetan Kowalski ◽  
Sławomir Krejszeff ◽  
Dariusz Kucharczyk
Keyword(s):  
Common Carp ◽  
Sperm Motility ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Suitable Solution ◽  
Computer Assisted Sperm Analysis

Monthly variation in sperm motility in common carp assessed using computer-assisted sperm analysis (CASA)

1996 ◽  
Vol 48 (6) ◽  
pp. 1210-1222 ◽  
Author(s):  
S. A. Christ ◽  
G. P. Toth ◽  
H. W. McCarthy ◽  
J. A. Torsella ◽  
M. K. Smith
Keyword(s):  
Common Carp ◽  
Sperm Motility ◽  
Computer Assisted ◽  
Monthly Variation ◽  
Sperm Analysis ◽  
Computer Assisted Sperm Analysis

scholarly journals Effects of chilled storage and pH of activating solution on different motility parameters in burbot (Lota lota) sperm

2018 ◽  
Vol 63 (No. 11) ◽  
pp. 429-434
Author(s):  
Zoltán Bokor ◽  
Balázs Csorbai ◽  
Levente Várkonyi ◽  
Zsolt Szári ◽  
Ferenc Fodor ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Chilled Storage ◽  
Straight Line ◽  
H 26 ◽  
Computer Assisted Sperm Analysis ◽  
Motility Parameters ◽  
Activating Solution

The effects of a simple saline solution prepared using two different pH (4.4 and 8.5) on sperm motility in burbot were investigated. Results were recorded during a 96-hour chilled storage (4°C) in 24-hour intervals. Measurements were focused on the detailed characteristics of motility using 12 parameters obtained from the Computer-assisted Sperm Analysis (CASA). Significantly higher progressive motility (pMOT), distance average path (DAP), distance curved line, distance straight line (DSL), average path velocity (VAP), curvilinear velocity, straight line velocity, and beat cross frequency (BCF) were observed with the activating solution buffered at pH 8.5 in comparison with pH 4.4. Already after 24 h a significant reduction was measured in pMOT (0 h: 49 ± 24%, 24 h: 12 ± 7%). Similar decreasing tendency was recorded only after 72 h in DAP (0 h: 26 ± 4 µm/s, 72 h: 19 ± 9 µm/s), DSL (0 h: 21 ± 5 µm/s, 72 h: 17 ± 8 µm/s), VAP (0 h: 59 ± 9 µm/s, 72 h: 43 ± 21 µm/s), and BCF (0 h: 28 ± 2 Hz, 72 h: 18 ± 10 Hz). The response of different investigated CASA parameters to different treatments varied in our experiments. According to our studies, numerous burbot sperm motility parameters are sensitive to chilled storage and to low pH of the activating solution. Our results could support the effective sperm quality assessment and successful artificial propagation process in burbot.

Effects of permethrin, cypermethrin and 3-phenoxybenzoic acid on rat sperm motility in vitro evaluated with computer-assisted sperm analysis

2010 ◽  
Vol 24 (2) ◽  
pp. 382-386 ◽  
Author(s):  
Chen Yuan ◽  
Cheng Wang ◽  
Shu-Qin Gao ◽  
Tian-Tian Kong ◽  
Lei Chen ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Computer Assisted Sperm Analysis

scholarly journals Evaluation of outer dense fiber-1 and -2 protein expression in asthenozoospermic infertile men

2015 ◽  
Vol 24 (2) ◽  
pp. 79-83
Author(s):  
Silvia W. Lestari ◽  
Dwi A. Pujianto ◽  
Purnomo Soeharso ◽  
Evelyn Loanda
Keyword(s):  
Protein Expression ◽  
Sperm Motility ◽  
Computer Assisted ◽  
Fibrous Sheath ◽  
Sperm Analysis ◽  
Straight Line ◽  
The Mean ◽  
Computer Assisted Sperm Analysis ◽  
And Control ◽  
Infertile Patients

Background: Most of male infertility are caused by defect in sperm motility (asthenozoospermia). The molecular mechanism of low sperm motility in asthenozoospermic patients has not been fully understood. Sperm motility is strongly related to the axoneme structure which is composed of microtubules and supported by outer dense fiber (ODF) and fibrous sheath (FS) protein. The objective of this study was to characterize the ODF (ODF1 and ODF2) expression in asthenozoospermic infertile male and control normozoospermic fertile male.Methods: Asthenozoospermic samples (n=18) were collected from infertile patients at Andrology Lab, Cipto Mangunkusumo Hospital Jakarta and control were taken from normozoospermic fertile donor (n=18). After motility analyses by computer-assisted sperm analysis (CASA), semen were divided into two parts, for Western blot and for immunocytochemistry analysis. Antibody against ODF1 and ODF2 protein were used in both analyses.Results: Analysis of ODF1 protein expression showed bands with molecular weight of ~30 kDa and ODF2 ~85 kDa. The mean band intensity of ODF1 and ODF2 protein were lower in the asthenozoospermic group (AG) compared to normozoospermic group (NG). Moreover, both ODF proteins were less intense and less localized in the AG than NG. Sperm motility was lower in AG, compared to control NG, i.e. average path velocity (VAP) = 32.07 ± 7.03 vs 37.58 ± 8.73 µm/s, p = 0.455; straight line velocity (VSL) = 24.17 ± 6.90 vs 27.61 ± 4.50 µm/s, p = 0.317 and curvilinear velocity (VCL) = 45.68 ± 7.91 vs 55.55 ± 16.40 µm/s, p = 0.099.Conclusion: There is down-regulation of ODF1 and ODF2 protein expression and less-compact localization in AG sperm compared to the NG. These changes might have caused disturbances in the sperm motility as observed in this study.

scholarly journals Effects of zearalenone, α-zearalenol, and genistein on boar sperm motility in vitro

2017 ◽  
Vol 62 (No. 10) ◽  
pp. 435-445 ◽  
Author(s):  
A. Krejcárková ◽  
P. Folková ◽  
O. Šimoník ◽  
M. Šašková ◽  
R. Krejčířová ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Toxicological Research ◽  
Computer Assisted Sperm Analysis ◽  
In Vitro Effects ◽  
Dose Dependent Increase ◽  
Dose Dependent

Genistein (GEN) and zearalenone (ZEA), environmental oestrogens commonly present in feedstuff for pigs, are known for their effects on reproductive functions. The aim was to verify the in vitro effects of 0.5–20 µM concentrations of GEN, ZEA and its metabolite α-zearalenol (α-ZOL) on pig sperm motility. A dose-dependent increase of the immotile sperm amount against fast and medium-fast sperm clusters was observed with all three oestrogens from the lowest concentrations tested. Individual CASA (computer-assisted sperm analysis) parameters of motile sperms seemed to be less sensitive indicators. This should be considered especially in toxicological research on a sperm model. Background of inconsistencies in to date-published papers is discussed. The results shift the effective concentrations of ZEA, α-ZOL, and GEN to values achievable in vivo and raises the questions of risk assessment of these compounds in pig reproduction.

scholarly journals Optimization of CASA-Mot Analysis of Donkey Sperm: Optimum Frame Rate and Values of Kinematic Variables for Different Counting Chamber and Fields

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1993
Author(s):  
Sabrina Gacem ◽  
Jaime Catalán ◽  
Anthony Valverde ◽  
Carles Soler ◽  
Jordi Miró
Keyword(s):  
High Resolution ◽  
Sperm Motility ◽  
Frame Rate ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Head Displacement ◽  
Straight Line ◽  
Counting Chamber ◽  
Computer Assisted Sperm Analysis ◽  
Lateral Head

In order to optimize the donkey sperm motility analysis by the CASA (Computer Assisted Sperm Analysis)-Mot system, twelve ejaculates were collected from six jackasses. Capillary loaded chamber (CLC), ISAS®D4C depths 10 and 20 µm, ISAS®D4C Leja 20 and drop displacement chamber (DDC), Spermtrack® (Spk) depths 10 and 20 µm were used. Sperm kinematic variables were evaluated using each chamber and a high-resolution camera capable of capturing a maximum of 500 frames/second (fps). The optimum frame rate (OFR) (defined according to curvilinear velocity—VCL) was dependent on chamber type. The highest OFR obtained was 278.46 fps by Spk20. Values for VCL, straight-line velocity (VSL), straightness (STR), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) were high in DDC and 10 µm depth. In both DDC 10 and 20 µm, the sperm velocities (VCL, VSL, VAP) and ALH values decreased significantly from the centre to the edges, while Wobble and BCF increased. No defined behavior was observed along the CLC. However, all the kinematic variables had a higher value in a highly concentrated sample, in both chamber types. In conclusion, analyzing a minimum of nine fields at 250 fps from the centre to the edges in Spk10 chamber using a dilution of 30 × 106 sperm/mL offers the best choice for donkey computerised sperm motility analysis.

16 COMPUTER-ASSISTED SPERM ANALYSIS SPERM PARAMETERS OF BOS TAURUS CRIOLLOS BULL SEMEN v. THOSE OF OTHER BOS TAURUS BREEDS IN ARGENTINA

2008 ◽  
Vol 20 (1) ◽  
pp. 88
Author(s):  
M. G. Lüssenhoff ◽  
G. Larraburu ◽  
R. Cavia ◽  
A. Garcia Guerra ◽  
G. M. Brogliatti
Keyword(s):  
Sperm Motility ◽  
Sperm Morphology ◽  
Bos Taurus ◽  
Hybrid Vigor ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Bull Semen ◽  
Significant Difference ◽  
Computer Assisted Sperm Analysis ◽  
Motility Parameters

Criollos is the oldest cattle breed in America and the world at large. The origin dates back to the first cattle brought by C. Columbus. They are well known for their toughness and longevity. Their genetic variability is another advantage to be taken into account in crossbreeding because it ensures high hybrid vigor. The objectives of the present study were to determine computer-assisted sperm analysis (CASA) motility parameters and sperm morphology of Criollos bull semen (brought from Parque Nacional Los Glaciares, Patagonia, Argentina) v. other Bos taurus semen. A total of 29 ejaculates of different adult bulls (Angus and Hereford) and 25 ejaculates of Criollos bulls were evaluated in an artificial insemination center between December 2004 and December 2005. Semen collection was done by electroejaculation (EE) in all breeds. Fresh semen was diluted in a semi-defined semen extender (Andromed; Minitüb, Tiefenbach, Germany) and frozen in an automatic freezer (Digicool, IMV, Paillette Crista, France). Stain morphology was done using eosin/nigrosin, and evaluated in an optic microscope with phase contrast. Parameters of volume, total concentration (CONC), motility %, motile progressive sperm %, velocity average path (VAP, μm s–1), velocity straight line (VSL, μm s–1), curvilinear velocity (VCL, μm s–1), and linearity (LIN, %) were determined by CASA (HTM-ceros 12.1, Berkley, CA, USA). The results obtained were analyzed statistically with a one-way ANOVA test and are summarized in Table 1. Results from the sperm motility analysis indicate that there were no significant differences (P > 0.05) in CONC, motility %, motile progressive %, or VSL between Criollos and other B. taurus bull semen. VAP and VCL rates were significantly higher in Criollos bull semen than in other B. taurus semen, and LIN was lower. Also, there was a significant difference in volume collected between both breeds. Regarding sperm morphology, Criollos bull semen is at the maximum limit acceptable for head defects (detached heads: 15%). These results suggest that Criollos bull semen is not different from other Bos taurus semen; however, it does show different velocity and linearity rates. Table 1. CASA sperm motility parameters for semen from Criollos v. other Bos taurus bulls This research was supported by Centro Genetico Bovino Eolia S.A.

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