scholarly journals Plasma amino acid profiles in dogs with inflammatory bowel disease

2019 ◽  
Vol 33 (4) ◽  
pp. 1602-1607 ◽  
Author(s):  
Yu Tamura ◽  
Hiroshi Ohta ◽  
Yumiko Kagawa ◽  
Tatsuyuki Osuga ◽  
Keitaro Morishita ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Amino Acid ◽  
Bowel Disease ◽  
Plasma Amino Acid ◽  
Amino Acid Profiles ◽  
Inflammatory Bowel

scholarly journals Novel, Objective, Multivariate Biomarkers Composed of Plasma Amino Acid Profiles for the Diagnosis and Assessment of Inflammatory Bowel Disease

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e31131 ◽  
Author(s):  
Tadakazu Hisamatsu ◽  
Susumu Okamoto ◽  
Masaki Hashimoto ◽  
Takahiko Muramatsu ◽  
Ayatoshi Andou ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Amino Acid ◽  
Bowel Disease ◽  
Plasma Amino Acid ◽  
Amino Acid Profiles ◽  
Inflammatory Bowel

scholarly journals Fecal Amino Acid Analysis in Newly Diagnosed Pediatric Inflammatory Bowel Disease: A Multicenter Case-Control Study

2021 ◽  
Author(s):  
Jasmijn Z Jagt ◽  
Eduard A Struys ◽  
Ibrahim Ayada ◽  
Abdellatif Bakkali ◽  
Erwin E W Jansen ◽  
...  
Keyword(s):  
Amino Acids ◽  
Inflammatory Bowel Disease ◽  
Amino Acid ◽  
Bowel Disease ◽  
Newly Diagnosed ◽  
Amino Acid Profiles ◽  
Inflammatory Bowel ◽  
Control Study ◽  
Pediatric Ibd

Abstract Background Fecal metabolomic profiles differ between pediatric inflammatory bowel disease (IBD) patients and controls and may provide new insights in the pathophysiology of IBD. The role of amino acids, however, is not fully elucidated. We aimed to assess fecal amino acid profiles in pediatric IBD. Methods In this case-control study, treatment-naïve, newly diagnosed pediatric IBD patients and a non-IBD control group, matched based on sex and age, were included in 2 tertiary centres. Fecal amino acid profiles were assessed using a targeted high-performance liquid chromatography technique. A random forest classifier method was used to develop a prediction model differentiating IBD from controls and predicting IBD phenotype. The association between IBD localization and amino acid concentrations was tested with ordinal regression models. Results We included 78 newly diagnosed IBD patients (40 Crohn’s disease [CD], 38 ulcerative colitis [UC]) and 105 controls. Patients with IBD could be differentiated from controls with an accuracy of 82% (sensitivity 63%, specificity 97%). Twenty-nine out of the 42 measured unique amino acids were included in the prediction model. Increased levels of tryptophan, taurine, alanine, ornithine, valine, histidine, and leucine were the most differentiating features. Children with CD and UC could be differentiated from the controls with an accuracy of 80% and 90%, respectively. Inflammatory bowel disease phenotype could not be predicted. Tryptophan, valine, and histidine levels were positively associated with more extended disease in UC patients (P < .05). Conclusions Fecal amino acids may enhance understanding of the role of host-microbial interactions in the pathophysiology of IBD and may evolve into biomarkers for pediatric IBD diagnostic and personalized medicine.

scholarly journals Fecal Amino Acid Profiles Exceed Accuracy of Serum Amino Acids in Diagnosing Pediatric Inflammatory Bowel Disease

2020 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Sofie Bosch ◽  
Sofia El Manouni el Hassani ◽  
Marina Brizzio Brentar ◽  
Ibrahim Ayada ◽  
Abdellatif Bakkali ◽  
...  
Keyword(s):  
Amino Acids ◽  
Inflammatory Bowel Disease ◽  
Amino Acid ◽  
Bowel Disease ◽  
Pediatric Inflammatory Bowel Disease ◽  
Serum Amino Acids ◽  
Amino Acid Profiles ◽  
Inflammatory Bowel

scholarly journals Profiling of Amino Acids in Urine Samples of Patients Suffering from Inflammatory Bowel Disease by Capillary Electrophoresis-Mass Spectrometry

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3345 ◽  
Author(s):  
Juraj Piestansky ◽  
Dominika Olesova ◽  
Jaroslav Galba ◽  
Katarina Marakova ◽  
Vojtech Parrak ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Inflammatory Bowel Disease ◽  
Capillary Electrophoresis ◽  
Amino Acid ◽  
Bowel Disease ◽  
Urine Samples ◽  
Simultaneous Action ◽  
Relative Standard ◽  
Inflammatory Bowel

Urine represents a convenient biofluid for metabolomic studies due to its noninvasive collection and richness in metabolites. Here, amino acids are valuable biomarkers for their ability to reflect imbalances of different biochemical pathways. An impact of amino acids on pathology, prognosis and therapy of various diseases, including inflammatory bowel disease (IBD), is therefore the subject of current clinical research. This work is aimed to develop a capillary electrophoresis-tandem mass spectrometry (CE-MS/MS) method for the quantification of the 20 proteinogenic amino acids in human urine samples obtained from patients suffering from IBD and treated with thiopurines. The optimized CE-MS/MS method, with minimum sample preparation (just “dilute and shoot”), exhibited excellent linearity for all the analytes (coefficients of determination were higher than 0.99), with inter-day and intra-day precision yielding relative standard deviations in the range of 0.91–15.12% and with accuracy yielding relative errors in the range of 85.47–112.46%. Total analysis time, an important parameter for the sample throughput demanded in routine practice, was shorter in ca. 17% when compared to established CE-MS methods. Favorable performance of the proposed CE-MS/MS method was also confirmed by the comparison with corresponding ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) method. Consistent data for the investigated amino acid metabolome were obtained using both methods. For the first time, the amino acid profiling by CE-MS approach was applied on the clinical IBD samples. Here, significant differences observed in the concentration levels of some amino acids between IBD patients undergoing thiopurine treatment and healthy volunteers could result from the simultaneous action of the disease and the corresponding therapy. These findings indicate that amino acids analysis could be a valuable tool for the study of mechanism of the IBD treatment by thiopurines.

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