Viral load and clinical manifestations of hepatitis E virus genotype 3 infections

2019 ◽  
Author(s):  
Sebastien Lhomme ◽  
Pierre Gallian ◽  
Chloé Dimeglio ◽  
Azzedine Assal ◽  
Florence Abravanel ◽  
...  
Keyword(s):  
Viral Load ◽  
Hepatitis E Virus ◽  
Clinical Manifestations ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype

scholarly journals Hepatitis E Virus Genotype 3 Strains in Domestic Pigs, Cameroon

2013 ◽  
Vol 19 (4) ◽  
pp. 686-688 ◽  
Author(s):  
Vanessa Salete de Paula ◽  
Matthias Wiele ◽  
Afegenwi H. Mbunkah ◽  
Achukwi M. Daniel ◽  
Manchang T. Kingsley ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype ◽  
Domestic Pigs

DETECTION AND PHYLOGENETIC CHARACTERIZATION OF HEPATITIS E VIRUS GENOTYPE 3 IN A CAPTIVE WILD BOAR IN THAILAND

2012 ◽  
Vol 43 (3) ◽  
pp. 640-644 ◽  
Author(s):  
Anuwat Wiratsudakul ◽  
Ladawan Sariya ◽  
Phirom Prompiram ◽  
Siriporn Tantawet ◽  
Duangkhamol Suraruangchai ◽  
...  
Keyword(s):  
Wild Boar ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype ◽  
Phylogenetic Characterization

scholarly journals No Evidence for Hepatitis E Virus Genotype 3 Susceptibility in Rats

2013 ◽  
Vol 19 (8) ◽  
pp. 1343-1345 ◽  
Author(s):  
Tian-Cheng Li ◽  
Yasushi Ami ◽  
Yuriko Suzaki ◽  
Naokazu Takeda ◽  
Wakita Takaji
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype

scholarly journals Comprehensive Molecular Approach for Characterization of Hepatitis E Virus Genotype 3 Variants

2018 ◽  
Vol 56 (5) ◽  
Author(s):  
Bo Wang ◽  
Dominik Harms ◽  
C. Patrick Papp ◽  
Sandra Niendorf ◽  
Sonja Jacobsen ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis E Virus ◽  
Phylogenetic Analyses ◽  
Hepatitis E ◽  
Full Length ◽  
Molecular Approach ◽  
Genotype 3 ◽  
Genome Sequences ◽  
Virus Genotype ◽  
Pcr Targeting

ABSTRACT Autochthonous hepatitis E virus genotype 3 (HEV-3) infections in industrialized countries are more frequent than previously assumed. HEV-3 is zoonotic and the causal pathogen of chronic hepatitis E. According to the latest classification of the family Hepeviridae , 10 designated HEV-3 subtypes (HEV-3a to HEV-3j) and 7 unassigned HEV-3 subtypes are proposed. In order to identify and characterize the HEV-3 variants in circulation, we developed a molecular approach combining a sensitive HEV-specific real-time reverse transcription-PCR (RT-PCR) targeting the overlapping region of HEV ORF2 and ORF3 (the ORF2/3 region) and two newly designed consensus nested RT-PCRs targeting the HEV ORF1 and ORF2 genes, respectively. Since complete genome sequences are required for new HEV-3 subtype assignment, we implemented a straightforward approach for full-length HEV-3 genome amplification. Twenty-nine human serum samples and six human feces samples from chronic hepatitis E patients were selected for evaluation of the system. Viral loads ranged from 1 × 10 4 to 1.9 × 10 10 copies/ml of serum and from 1.8 × 10 4 to 1 × 10 12 copies/g of feces. Sequence and phylogenetic analyses of partial ORF1 and ORF2 sequences showed that HEV strains had considerable genetic diversity and clustered into the HEV-3c (29/35), HEV-3e (2/35), HEV-3f (2/35), and unassigned HEV-3 (2/35) subtypes. Moreover, from these strains, three full-length HEV-3 genome sequences were generated and characterized. DE/15-0030 represents a typical HEV-3c strain (95.7% nucleotide identity to wbGER27), while DE/15-0031 and SW/16-0282 have <89.2% homology to known HEV-3 strains and are phylogenetically divergent, indicating novel HEV-3 subtypes. In summary, our approach will significantly facilitate the detection, quantification, and determination of HEV-3 strains and will thus help to improve molecular diagnostics and our knowledge of HEV diversity and evolution.

scholarly journals Hallmarks of liver lesions in pigs naturally infected by hepatitis E virus genotype 3

2018 ◽  
Vol 38 (1) ◽  
pp. 65-70
Author(s):  
Alex J.S. de Souza ◽  
Andreza P. Malheiros ◽  
Manoel do C.P. Soares ◽  
Michele S. Gomes-Gouvêa ◽  
João R.R. Pinho ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Molecular Techniques ◽  
Human Consumption ◽  
Liver Lesions ◽  
Genotype 3 ◽  
Virus Genotype ◽  
Liver Histopathology ◽  
Significant Difference ◽  
Group 2

ABSTRACT: Histopathological evaluation of liver from 33 pigs slaughtered for human consumption in Amazon region, previously tested by serology and molecular techniques for hepatitis E virus infection (HEV), was analysed in three groups: Group 1, negative for both HEV-RNA and anti-HEV IgG (n=10); Group 2, positive for HEV-RNA (n=13); Group 3, positive for anti-HEV IgG (n=10). Group 2 showed a significant difference among the groups for liver lesions such as lobular activity (P=0.007), periportal interface hepatitis (P=0.004), portal inflammation (P=0.028) hepatitis with lobular, portal and periportal interface activity (P=0.001). HEV detection by immunohistochemistry was performed and 3 of 6 samples of group 2 were positive. Pigs naturally infected by HEV genotype 3 present microscopic necroinflammatory liver lesions similar to HEV in humans. Liver histopathology showed be important in the diagnosis of active asymptomatic HEV infection in pigs slaughtered for human consumption because hepatic liver lesions may present distinct profiles according to molecular and serological diagnosis and in this sense, histopathology and immunohistochemistry may be an important complementary diagnostic tool.

scholarly journals Hepatitis E virus genotype 3 and capsid protein in the blood and urine of immunocompromised patients

2019 ◽  
Vol 78 (3) ◽  
pp. 232-240 ◽  
Author(s):  
Olivier Marion ◽  
Nicolas Capelli ◽  
Sebastien Lhomme ◽  
Martine Dubois ◽  
Mélanie Pucelle ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Immunocompromised Patients ◽  
Genotype 3 ◽  
Virus Genotype

scholarly journals Detection and Characterization of Hepatitis E Virus Genotype 3 in Wastewater and Urban Surface Waters in Germany

2020 ◽  
Vol 12 (2) ◽  
pp. 137-147 ◽  
Author(s):  
Sophia Beyer ◽  
Regine Szewzyk ◽  
Regina Gnirss ◽  
Reimar Johne ◽  
Hans-Christoph Selinka
Keyword(s):  
Surface Waters ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Urban Surface ◽  
Genotype 3 ◽  
Virus Genotype

scholarly journals First detection of hepatitis E virus genotype 3 as a common infectious agent in patients with chronic liver damage in Mexico

2019 ◽  
Vol 18 (4) ◽  
pp. 571-577 ◽  
Author(s):  
Oliver Viera-Segura ◽  
Mauricio Realpe-Quintero ◽  
Arturo Panduro ◽  
Sonia Roman ◽  
Alexis Jose-Abrego ◽  
...  
Keyword(s):  
Liver Damage ◽  
Hepatitis E Virus ◽  
Infectious Agent ◽  
Hepatitis E ◽  
Chronic Liver ◽  
Genotype 3 ◽  
Virus Genotype ◽  
Chronic Liver Damage

scholarly journals Evolutionary study of potentially zoonotic hepatitis E virus genotype 3 from swine in Northeast Brazil

2019 ◽  
Vol 114 ◽  
Author(s):  
Edmilson Ferreira de Oliveira-Filho ◽  
Debora RL dos Santos ◽  
Ricardo Durães-Carvalho ◽  
Adalúcia da Silva ◽  
Gustavo Barbosa de Lima ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Northeast Brazil ◽  
Genotype 3 ◽  
Virus Genotype ◽  
Evolutionary Study
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