scholarly journals Gene and protein expression in human megakaryocytes derived from induced pluripotent stem cells

2021 ◽  
Author(s):  
Kai Kammers ◽  
Margaret A Taub ◽  
Rasika A Mathias ◽  
Lisa R Yanek ◽  
Kanika Kanchan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Protein Expression ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Gene And Protein Expression ◽  
Induced Pluripotent

Characteristics of Porcine Induced Pluripotent Stem Cells in their MHC Protein Expression

2012 ◽  
Vol 94 (10S) ◽  
pp. 605
Author(s):  
K.-M. Park ◽  
H.-S. Nam ◽  
S.-M. Park ◽  
S.-H. Cha ◽  
I.-H. Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Protein Expression ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals Apolipoprotein E expression pattern in human induced pluripotent stem cells during in vitro neural induction

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 353
Author(s):  
Hyunah Lee ◽  
Paulina Nowosiad ◽  
Lucia M. Dutan Polit ◽  
Jack Price ◽  
Deepak P. Srivastava ◽  
...  
Keyword(s):  
Stem Cells ◽  
Apolipoprotein E ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Neural Differentiation ◽  
Neural Induction ◽  
Peripheral Tissues ◽  
Gene And Protein Expression ◽  
Induced Pluripotent

Apolipoprotein E (APOE) is a multifunctional protein that plays significant roles in important cellular mechanisms in peripheral tissues and is as well expressed in the central nervous system, notably by adult neural stem cells (NSCs) in the hippocampus. Evidence from animal studies suggest that APOE is critical for adult NSC maintenance. However, whether APOE has the potential to play a similar role in human NSCs has not been directly investigated. To address this question, we conducted a focused study characterising APOE gene and protein expression in an in vitro model of neural differentiation utilising human induced pluripotent stem cells. We found that APOE gene expression was dramatically decreased as the cells became more differentiated, indicating that APOE expression levels reflect the degree of cellular differentiation during neural induction. Furthermore, qualitative analysis results of immunocytochemistry showed that intracellular localisation of APOE protein becomes more pronounced as neural differentiation progresses. Taken together, our findings suggest a potential role for APOE in human NSC maintenance and justify further investigations being carried out to understand whether changes in APOE levels can directly impact the neurogenic capacity of human stem cells.

scholarly journals Thyroid Hormone Effect on the Differentiation of Human Induced Pluripotent Stem Cells into Hepatocyte-Like Cells

2021 ◽  
Vol 14 (6) ◽  
pp. 544
Author(s):  
Mariia S. Bogacheva ◽  
Margarita A. Bystriakova ◽  
Yan-Ru Lou
Keyword(s):  
Stem Cells ◽  
Thyroid Hormone ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Perinatal Period ◽  
Hormone Effect ◽  
Gene And Protein Expression ◽  
Dependent Inhibition ◽  
Induced Pluripotent

Human induced pluripotent stem cells (hiPSCs) hold great potential as an unlimited source for obtaining hepatocyte-like cells (HLCs) for drug research. However, current applications of HLCs have been severely limited by the inability to produce mature hepatocytes from hiPSCs in vitro. Thyroid hormones are one of the hormones that surge during the perinatal period when liver maturation takes place. Here we assessed the influence of thyroid hormone on hepatic progenitor differentiation to HLCs. We analyzed gene and protein expression of early and late hepatic markers and demonstrated the selective activity of thyroid hormone on different genes. Particularly, we demonstrated thyroid hormone-dependent inhibition of the fetal hepatic marker AFP. Our study sheds light on the role of thyroid hormone during liver differentiation and maturation.

scholarly journals Apolipoprotein E expression pattern in human induced pluripotent stem cells during in vitro neural induction

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 353
Author(s):  
Hyunah Lee ◽  
Paulina Nowosiad ◽  
Lucia M. Dutan Polit ◽  
Jack Price ◽  
Deepak P. Srivastava ◽  
...  
Keyword(s):  
Stem Cells ◽  
Apolipoprotein E ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Neural Differentiation ◽  
Neural Induction ◽  
Peripheral Tissues ◽  
Gene And Protein Expression ◽  
Induced Pluripotent

Apolipoprotein E (APOE) is a multifunctional protein that plays significant roles in important cellular mechanisms in peripheral tissues and is as well expressed in the central nervous system, notably by adult neural stem cells (NSCs) in the hippocampus. Evidence from animal studies suggest that APOE is critical for adult NSC maintenance. However, whether APOE has the potential to play a similar role in human NSCs has not been directly investigated. To address this question, we conducted a focused study characterising APOE gene and protein expression in an in vitro model of neural differentiation utilising human induced pluripotent stem cells. We found that APOE gene expression was dramatically decreased as the cells became more differentiated, indicating that APOE expression levels reflect the degree of cellular differentiation during neural induction. Furthermore, qualitative analysis results of immunocytochemistry showed that intracellular localisation of APOE protein becomes more pronounced as neural differentiation progresses. Taken together, our findings suggest a potential role for APOE in human NSC maintenance and justify further investigations being carried out to understand whether changes in APOE levels can directly impact the neurogenic capacity of human stem cells.

Directed differentiation of mouse-induced pluripotent stem cells generates dopaminergic nerve

2010 ◽  
Vol 34 (8) ◽  
pp. S36-S36
Author(s):  
Ping Duan ◽  
Xuelin Ren ◽  
Wenhai Yan ◽  
Xuefei Han ◽  
Xu Yan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Directed Differentiation ◽  
Induced Pluripotent
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