Proof of concept of a new scale for the harmonization and the standardization of the ETP‐based APC resistance

Diffraction intensities from intermetallic compounds are, in the kinematic approximation, proportional to the scattering amplitude from the element doing the scattering. More detailed calculations have shown that site symmetry and occupation by various atom species also affects the intensity in a diffracted beam. [1] Hence, by measuring the intensities of beams, or their ratios, the occupancy can be estimated. Measurement of the intensity values also allows structure calculations to be made to determine the spatial distribution of the potentials doing the scattering. Thermal effects are also present as a background contribution. Inelastic effects such as loss or absorption/excitation complicate the intensity behavior, and dynamical theory is required to estimate the intensity value.The dynamic range of currents in diffracted beams can be 104or 105:1. Hence, detection of such information requires a means for collecting the intensity over a signal-to-noise range beyond that obtainable with a single film plate, which has a S/N of about 103:1. Although such a collection system is not available currently, a simple system consisting of instrumentation on an existing STEM can be used as a proof of concept which has a S/N of about 255:1, limited by the 8 bit pixel attributes used in the electronics. Use of 24 bit pixel attributes would easily allowthe desired noise range to be attained in the processing instrumentation. The S/N of the scintillator used by the photoelectron sensor is about 106 to 1, well beyond the S/N goal. The trade-off that must be made is the time for acquiring the signal, since the pattern can be obtained in seconds using film plates, compared to 10 to 20 minutes for a pattern to be acquired using the digital scan. Parallel acquisition would, of course, speed up this process immensely.

Download Full-text

Remote Echocardiography: Proof of Concept for Support of National Disasters, Combat and Humanitarian Mission

PsycEXTRA Dataset ◽

10.1037/e452292006-001 ◽

2002 ◽

Author(s):

Sheri Yvonne Nottestad Boyd ◽

Linda L. Huffer ◽

Terry D. Bauch ◽

James L. Furgerson

Keyword(s):

Proof Of Concept ◽

Humanitarian Mission

Download Full-text

Model, simulation and experiments for a Buoyancy Organic Rankine Cycle

The European Physical Journal Applied Physics ◽

10.1051/epjap/2020200113 ◽

2020 ◽

Vol 92 (1) ◽

pp. 10906

Author(s):

Jeroen Schoenmaker ◽

Pâmella Gonçalves Martins ◽

Guilherme Corsi Miranda da Silva ◽

Julio Carlos Teixeira

Keyword(s):

Model Simulation ◽

Organic Rankine Cycle ◽

Thermodynamic Cycle ◽

Rankine Cycle ◽

Test Body ◽

Working Fluid ◽

Proof Of Concept ◽

Energy Scenario ◽

New Type ◽

Fluid Reservoir

Organic Rankine Cycle (ORC) systems are increasingly gaining relevance in the renewable and sustainable energy scenario. Recently our research group published a manuscript identifying a new type of thermodynamic cycle entitled Buoyancy Organic Rankine Cycle (BORC) [J. Schoenmaker, J.F.Q. Rey, K.R. Pirota, Renew. Energy 36, 999 (2011)]. In this work we present two main contributions. First, we propose a refined thermodynamic model for BORC systems accounting for the specific heat of the working fluid. Considering the refined model, the efficiencies for Pentane and Dichloromethane at temperatures up to 100 °C were estimated to be 17.2%. Second, we show a proof of concept BORC system using a 3 m tall, 0.062 m diameter polycarbonate tube as a column-fluid reservoir. We used water as a column fluid. The thermal stability and uniformity throughout the tube has been carefully simulated and verified experimentally. After the thermal parameters of the water column have been fully characterized, we developed a test body to allow an adequate assessment of the BORC-system's efficiency. We obtained 0.84% efficiency for 43.8 °C working temperature. This corresponds to 35% of the Carnot efficiency calculated for the same temperature difference. Limitations of the model and the apparatus are put into perspective, pointing directions for further developments of BORC systems.

Download Full-text

Indocyanine green-augmented diode laser therapy of telangiectatic and reticular leg veins: a clinical proof-of-concept trial

Endoskopie heute ◽

10.1055/s-0032-1308757 ◽

2012 ◽

Vol 25 (01) ◽

Cited By ~ 1

Author(s):

A Klein ◽

G Shafirstein ◽

E Kohl ◽

W Bäumler ◽

M Landthaler ◽

...

Keyword(s):

Indocyanine Green ◽

Laser Therapy ◽

Diode Laser ◽

Proof Of Concept

Download Full-text

Evaluation einer als Proof-of-Concept entwickelten MRT-kompatiblen und röntgentransparenten Knochenbohrmaschine

RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren ◽

10.1055/s-0032-1311173 ◽

2012 ◽

Vol 184 (S 01) ◽

Author(s):

FV Güttler ◽

K Winterwerber ◽

C Gross ◽

A Heinrich ◽

M de Bucourt ◽

...

Keyword(s):

Proof Of Concept

Download Full-text

The Incidence of Factor V Leiden in a Normal Irish Population and Its Relationship to the Laboratory Diagnosis of APC Resistance

Thrombosis and Haemostasis ◽

10.1055/s-0037-1614545 ◽

1999 ◽

Vol 81 (04) ◽

pp. 661-663 ◽

Cited By ~ 2

Author(s):

Joseph Vaughan ◽

Cariosa Power ◽

Catherine Nolan ◽

Don McCarthy ◽

Ivan Shirley

Keyword(s):

Factor V Leiden ◽

Laboratory Diagnosis ◽

Factor V ◽

Irish Population ◽

Apc Resistance

Download Full-text

Besser als Genexpressionstests?

Onkologische Welt ◽

10.1055/s-0038-1649303 ◽

2018 ◽

Vol 09 (02) ◽

pp. 65-66

Author(s):

Alexander Kretzschmar

Keyword(s):

New York ◽

Tumor Cells ◽

Circulating Tumor Cells ◽

Proof Of Concept ◽

Zirkulierende Tumorzellen

Zirkulierende Tumorzellen (Circulating Tumor Cells; CTC) sind Prädiktoren von Spät-rezidiven bei Frauen mit einem Hormonrezeptor-positiven (HR-positiv), HER2-negativen frühen Mammakarzinom. Dies ergab eine Proof-of-Concept-Studie, die von Dr. Joseph Sparano, New York, auf dem SABCS 2017 vorgestellt wurde. Spätrezidive machen etwa die Hälfte aller Östrogenrezeptor-positiven Rezidive aus. Genexpressionstests können zwar das Risiko eines Rezidivs insgesamt quantifizieren. Sparanos Arbeitsgruppe glaubt aber, dass CTC bessere Biomarker für Spätrezidive sind.

Download Full-text

“Pseudo Homozygous” Activated Protein C Resistance due to Double Heterozygous Factor V Defects (Factor V Leiden Mutation and Type I Quantitative Factor V Defect) Associated with Thrombosis: Report of Two Cases Belonging to Two Unrelated Kindreds

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650290 ◽

1996 ◽

Vol 75 (03) ◽

pp. 422-426 ◽

Cited By ~ 53

Author(s):

Paolo Simioni ◽

Alberta Scudeller ◽

Paolo Radossi ◽

Sabrina Gavasso ◽

Bruno Girolami ◽

...

Keyword(s):

Protein C ◽

Dna Analysis ◽

Activated Protein C ◽

Factor V Leiden ◽

Type I ◽

Factor V ◽

Factor V Leiden Mutation ◽

Thrombotic Risk ◽

Apc Resistance ◽

Quantitative Factor

SummaryTwo unrelated patients belonging to two Italian kindreds with a history of thrombotic manifestations were found to have a double heterozygous defect of factor V (F. V), namely type I quantitative F. V defect and F. V Leiden mutation. Although DNA analysis confirmed the presence of a heterozygous F. V Leiden mutation, the measurement of the responsiveness of patients plasma to addition of activated protein C (APC) gave results similar to those found in homozygous defects. It has been recently reported in a preliminary form that the coinheritance of heterozygous F. V Leiden mutation and type I quantitative F. V deficiency in three individuals belonging to the same family resulted in the so-called pseudo homozygous APC resistance with APC sensitivity ratio (APC-SR) typical of homozygous F. V Leiden mutation. In this study we report two new cases of pseudo homozygous APC resistance. Both patients experienced thrombotic manifestations. It is likely that the absence of normal F. V, instead of protecting from thrombotic risk due to heterozygous F. V Leiden mutation, increased the predisposition to thrombosis since the patients became, in fact, pseudo-homozygotes for APC resistance. DNA-analysis is the only way to genotype a patient and is strongly recommended to confirm a diagnosis of homozygous F. V Leiden mutation also in patients with the lowest values of APC-SR. It is to be hoped that no patient gets a diagnosis of homozygous F. V Leiden mutation based on the APC-resi-stance test, especially when the basal clotting tests, i.e., PT and aPTT; are borderline or slightly prolonged.

Download Full-text

Elevated Levels of Prothrombin Activation Fragment 1+2 in Plasma from Patients with Heterozygous Arg506 to Gin Mutation in the Factor V Gene (APC-Resistance) and/or Inherited Protein S Deficiency

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650259 ◽

1996 ◽

Vol 75 (02) ◽

pp. 270-274 ◽

Cited By ~ 57

Author(s):

Benget Zöller ◽

Johan Holm ◽

Peter Svensson ◽

Björn Dahlbäck

Keyword(s):

Plasma Concentration ◽

Protein C ◽

Protein S ◽

Factor V ◽

Protein S Deficiency ◽

Apc Resistance ◽

S Deficiency ◽

Increased Risk ◽

Factor V Gene ◽

V Gene

SummaryInherited resistance to activated protein C (APC-resistance), caused by a point mutation in the factor V gene leading to replacement of Arg(R)506 with a Gin (Q), and inherited protein S deficiency are associated with functional impairment of the protein C anticoagulant system, yielding lifelong hypercoagulability and increased risk of thrombosis. APC-resistance is often an additional genetic risk factor in thrombosis-prone protein S deficient families. The plasma concentration of prothrombin fragment 1+2 (F1+2), which is a marker of hyper-coagulable states, was measured in 205 members of 34 thrombosis-prone families harbouring the Arg506 to Gin mutation (APC-resistance) and/or inherited protein S deficiency. The plasma concentration of F1+2 was significantly higher both in 38 individuals carrying the FV:Q506 mutation in heterozygous state (1.7 ± 0.7 nM; mean ± SD) and in 48 protein S deficient cases (1.9 ± 0.9 nM), than in 100 unaffected relatives (1.3 ±0.5 nM). Warfarin therapy decreased the F1+2 levels, even in those four patients who had combined defects (0.5 ± 0.3 nM). Our results agree with the hypothesis that individuals with APC-resistance or protein S deficiency have an imbalance between pro- and anti-coagulant forces leading to increased thrombin generation and a hypercoagulable state.

Download Full-text

Phenotyping and Genotyping of Coagulation Factor V Leiden

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650258 ◽

1996 ◽

Vol 75 (02) ◽

pp. 267-269 ◽

Cited By ~ 27

Author(s):

H Engel ◽

L Zwang ◽

H H D M van Vliet ◽

J J Michiles ◽

J Stibbe ◽

...

Keyword(s):

Factor V Leiden ◽

Coagulation Factor ◽

Diagnostic Value ◽

Resistance Test ◽

Amplification Refractory Mutation System ◽

Factor V ◽

Chain Reactions ◽

Apc Resistance ◽

Specificity And Sensitivity ◽

Coagulation Factor V

SummaryThe currently used activated Protein C resistance test demonstrated to be of limited diagnostic value for the detection of the mutant Factor V Leiden. Moreover, this assay is not useful for patients under anticoagulant therapy. A modification of the APC resistance test, applying Factor V deficient plasma is described which demonstrates a specificity and sensitivity of 1.0. The superiority of the modified APC resistance test over the existing APC resistance test was verified by genotyping.For that purpose, the Amplification Refractory Mutation System (ARMS) was applied to the detection of the G to A mutation at position 1691 in the gene encoding coagulation Factor V. The mutation at that position could be easily detected by using each of two allele-specific oligonucleotide primers concomitantly with one common primer in two separate polymerase chain reactions, thereby amplifying a fragment of 186 base-pairs of the Factor V gene.

Download Full-text