scholarly journals Engineering a protein Z‐dependent protease inhibitor ( ZPI ) mutant as a novel antagonist of ZPI anticoagulant function for hemophilia treatment

2019 ◽  
Vol 17 (10) ◽  
pp. 1655-1660 ◽  
Author(s):  
Xin Huang
Keyword(s):  
Protease Inhibitor ◽  
Protein Z ◽  
Anticoagulant Function

scholarly journals Lipid oxidation inactivates the anticoagulant function of protein Z-dependent protease inhibitor (ZPI)

2017 ◽  
Vol 292 (35) ◽  
pp. 14625-14635 ◽  
Author(s):  
Xin Huang ◽  
Baoxin Liu ◽  
Yidong Wei ◽  
Ryan Beyea ◽  
Han Yan ◽  
...  
Keyword(s):  
Protease Inhibitor ◽  
Lipid Oxidation ◽  
Protein Z ◽  
Anticoagulant Function

Protein Z/protein Z-dependent protease inhibitor system in human non-small-cell lung cancer tissue

2012 ◽  
Vol 129 (4) ◽  
pp. e92-e96 ◽  
Author(s):  
Ewa Sierko ◽  
Marek Z. Wojtukiewicz ◽  
Lech Zimnoch ◽  
Krystyna Ostrowska-Cichocka ◽  
Piotr Tokajuk ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Protease Inhibitor ◽  
Small Cell Lung Cancer ◽  
Small Cell ◽  
Cancer Tissue ◽  
Lung Cancer Tissue ◽  
Small Cell Lung ◽  
Protein Z ◽  
Inhibitor System ◽  
Z Protein

Plasma Concentrations of Protein Z and Protein Z-Dependent Protease Inhibitor in Thalassemia Major Patients

2019 ◽  
Vol 1 (12) ◽  
pp. 1060-1064
Author(s):  
Majid Ghazanfari ◽  
Mohammad Ali Jalali Far ◽  
Saeed Shirali ◽  
Zari Tahannejad Asadi
Keyword(s):  
Protease Inhibitor ◽  
Plasma Concentrations ◽  
Thalassemia Major ◽  
Protein Z

Thrombin-Releasable Protein Z-Dependent Protease Inhibitor Identified in Platelets and Is Likely Due to Uptake from Plasma.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 1751-1751
Author(s):  
Xin Han ◽  
Martin H. Nguyen ◽  
Michael Fernandez ◽  
George J. Broze ◽  
Carlos E. Bueso-Ramos
Keyword(s):  
Bone Marrow ◽  
Western Blot ◽  
Protease Inhibitor ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Factor Xa ◽  
Human Populations ◽  
Rt Pcr ◽  
Protein Z ◽  
Platelet Factor

Abstract Protein Z (PZ) is a vitamin K-dependent protein, whereas PZ-dependent protease inhibitor (ZPI) is a member of the serine protease inhibitor superfamily. ZPI rapidly inhibits factor Xa in the presence of PZ, calcium, and phospholipids and inhibits factor XIa in a PZ-independent fashion (Blood2000; 96:3049–3055). PZ circulates as a complex with ZPI in plasma and deletion of either the PZ or the ZPI gene is associated with the prothrombotic phenotype in mice. In addition, W303X or R67X nonsense mutations in the ZPI gene are reportedly associated with deep venous thrombosis in certain human populations. Western blot analysis of platelets stimulated with thrombin (0–200 mU/mL) showed they contained and released ZPI (approximately 200 ng/109 platelets) with the same molecular weight as plasma ZPI (72 kDa). The majority of the ZPI was released within 1 min. by 25 mU/mL thrombin. PZ was not detected in platelets by western blot analysis. Immunohistochemical staining using a monoclonal anti-ZPI antibody demonstrated a cytoplasmic fine granular staining pattern in maturing megakaryocytes in bone marrow aspirates and in circulating platelets, suggesting that ZPI may be stored in alpha granules. ZPI mRNA, however, was not detected by reverse transcriptase polymerase chain reaction (RT-PCR) in platelets or bone marrow aspirates, but was detected in human liver cDNA. RT-PCR for platelet factor 4 and glyceraldehyde 3-phosphate dehydrogenase mRNA showed amplified products with expected sizes. In conclusion, thrombin-releasable ZPI, but not PZ, is present in platelets and is most likely derived from the uptake of ZPI from plasma. ZPI released from activated platelets may play a role in the regulation of local coagulation at a site of injury.

scholarly journals Protein Z-dependent protease inhibitor and protein Z in peripheral arterial disease patients

2009 ◽  
Vol 7 (5) ◽  
pp. 731-735 ◽  
Author(s):  
F. SOFI ◽  
F. CESARI ◽  
Y. TU ◽  
G. PRATESI ◽  
R. PULLI ◽  
...  
Keyword(s):  
Peripheral Arterial Disease ◽  
Protease Inhibitor ◽  
Arterial Disease ◽  
Protein Z ◽  
Peripheral Arterial
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