ODAM promotes junctional epithelium‐related gene expression via activation of WNT1 signaling pathway in an ameloblast‐like cell line ALC

2021 ◽  
Author(s):  
Danyang Song ◽  
Sui Yang ◽  
Tao Tan ◽  
Ruijie Wang ◽  
Zeyun Ma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Signaling Pathway ◽  
Junctional Epithelium ◽  
Related Gene

4-Nitrophenol exposure alters the AhR signaling pathway and related gene expression in the rat liver

2016 ◽  
Vol 37 (2) ◽  
pp. 150-158 ◽  
Author(s):  
Ruonan Li ◽  
Meiyan Song ◽  
Zhi Li ◽  
Yansen Li ◽  
Gen Watanabe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signaling Pathway ◽  
Rat Liver ◽  
Related Gene

scholarly journals A lymph node metastatic mouse model reveals alterations of metastasis-related gene expression in metastatic human oral carcinoma sublines selected from a poorly metastatic parental cell line

Cancer ◽  
2002 ◽  
Vol 95 (8) ◽  
pp. 1663-1672 ◽  
Author(s):  
Xin Zhang ◽  
Yanna Liu ◽  
Michael Z. Gilcrease ◽  
Xiao H. Yuan ◽  
Gary L. Clayman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lymph Node ◽  
Mouse Model ◽  
Cell Line ◽  
Parental Cell ◽  
Oral Carcinoma ◽  
Parental Cell Line ◽  
Related Gene

scholarly journals Melatonin Receptor 2 Promotes Lipid Metabolism of Cumulus-oocyte Complexes via the cAMP/PKA Pathway

2021 ◽  
Author(s):  
Jun-Xue Jin ◽  
Hong-Di Cui ◽  
Chao-Qian Jiang ◽  
Zi-Cheng Qi ◽  
Ya Bian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Energy Source ◽  
Lipid Metabolism ◽  
Signaling Pathway ◽  
Oocyte Maturation ◽  
Expression Patterns ◽  
Cumulus Cells ◽  
Related Gene ◽  
Melatonin Receptor ◽  
Significant Difference

Abstract Background: The importance of the processes of lipogenesis and lipolysis in providing an essential energy source during oocyte maturation is increasingly being recognized. Recent our studies have demonstrated that melatonin up-regulated lipid metabolism during oocyte maturation. Nevertheless, there is still limited information regarding the underlying molecular mechanisms of action of melatonin on lipid metabolism in porcine cumulus-oocyte complexes (COCs). Here, our aim was to investigate the effect of melatonin on COCs, and the melatonin receptor-mediated lipid metabolism signaling pathway.Materials/methods: To determine the melatonin-mediated lipolysis pathway in cumulus cells, COCs were treated with melatonin and the correlated metabolic responses were assessed using melatonin receptor-mediated signaling.Results: The results showed that exposure of COCs to melatonin during in vitro maturation significantly increased cumulus expansion index, blastocyst formation rate and total cell numbers/blastocyst, although nuclear maturation was no significant difference. The levels of proteins MT1, MT2, Gsα, PKA, and lipolysis-related factors (AGTL, HSL, PLIN A+B) were significantly increased by melatonin supplementation, and this effect was inhibited by simultaneous treatment with melatonin antagonists (luzindole or 4P-PDOT), although 4P-PDOT treatment did not completely block the effect of melatonin on MT1. Further, the gene expression patterns reflected their relevant protein levels in cumulus cells. Melatonin-mediated lipolysis could significantly reduce lipid droplets (LDs) numbers and increase fatty acid (FA) production and ATP levels by increasing the β-oxidation-related gene expression in cumulus cells. Simultaneously, melatonin significantly increased the amount of LDs, FAs, ATP, and enhanced the lipid metabolism-related gene expression in oocytes. Finally, the oocyte quality was improved by increasing GDF9, BMP15 and GSH and decreasing ROS levels.Conclusion: These findings revealed that the MT2-mediated cAMP/PKA signaling pathway promotes intracellular lipolysis and FA production in cumulus cells, which provided an essential energy source for COCs development.

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