scholarly journals A marine Chlamydomonas sp. emerging as an algal model

2020 ◽  
Author(s):  
David Carrasco Flores ◽  
Markus Fricke ◽  
Valentin Wesp ◽  
Daniel Desirò ◽  
Anja Kniewasser ◽  
...  
Keyword(s):  
1993 ◽  
Vol 44 (3) ◽  
pp. 389 ◽  
Author(s):  
RV Hyne ◽  
A Padovan ◽  
DL Parry ◽  
SM Renaud

The effects of two synthetic diets and four algal diets on the survival and fecundity of Moinodaphnia macleayi for use in life-cycle toxicity tests were determined. The synthetic foods were a suspension of blended cichlid fish pellets with powdered alfalfa, either prepared fresh or fermented in natural water for 3 days. The algae, isolated from the habitat of M. rnacleayi in the Alligator Rivers Region, were Selenastrum sp., Ankistrodesmus (falcatus?), Scenedesmus quadricauda and a Chlamydomonas sp. Each alga was tested alone or as a supplement to the fermented synthetic food. The diet that supported the greatest number of young and the highest survival rate over a 5-day period was a combination of the fermented synthetic food and Chlamydomonas sp. Analysis of the fatty acid composition of the lipid fraction of the four algal species showed that Chlamydomonas sp., Selenastrum sp. and Scenedesmus quadricauda had significant concentrations of the highly unsaturated fatty acids 18 : 2(n-6), 18 : 3(n-6) and 18: 3(n-3). In terms of size and dry weight, Chlamydomonas sp. was the smallest of the algal cells studied. The toxicity of uranium to M. macleayi survival, determined under standard conditions, was a lowest-observed-effect concentration (LOEC) of 200 �g L-1 after 48 h and 25 �g L-1 after 5 days.


2019 ◽  
Vol 272 ◽  
pp. 281-287 ◽  
Author(s):  
Ramachandran Sivaramakrishnan ◽  
Subramaniyam Suresh ◽  
Aran Incharoensakdi

Author(s):  
Marina Cvetkovska ◽  
Beth Szyszka-Mroz ◽  
Nina Malczewski ◽  
David Smith ◽  
Norman P. A. Huner

The Antarctic alga Chlamydomonas sp. UWO241 is an obligate psychrophile that thrives in the cold but is unable to survive at moderate, seemingly innocuous temperatures. We dissect the responses of UWO241 to temperature stress using global metabolomic approaches. UWO241 exhibits slow growth at 4°C, a temperature closest to its natural habitat, and faster growth at higher temperatures of 10-15°C. We demonstrate that the slower growth-rate characteristic of UWO241 at 4⁰C is not necessarily a hallmark of stress. UWO241 constitutively accumulates high levels of protective metabolites including soluble sugars, polyamines and antioxidants at a range of steady-state temperatures. In contrast, the mesophile Chlamydomonas reinhardtii accumulates these metabolites only during cold stress. Despite low growth rates, 4°C-grown UWO241 cultures had a higher capacity to respond to heat stress (24°C) and accumulated increased amounts of antioxidants, lipids and soluble sugars, when compared to cultures grown at 10-15°C. We conclude that the slower growth rate and the unique psychrophilic physiological characteristic of UWO241 grown at 4⁰C result in a permanently re-routed steady-state metabolism, which contributes to its increased resistance to heat stress. Our work adds to the growing body of research on temperature stress in psychrophiles, many of which are threatened by climate change.


2015 ◽  
Vol 39 (1) ◽  
pp. 151-157 ◽  
Author(s):  
Eun Jae Kim ◽  
Woongsic Jung ◽  
Suyoun Lim ◽  
Sanghee Kim ◽  
Se Jong Han ◽  
...  

2019 ◽  
Vol 141 ◽  
pp. 1-9 ◽  
Author(s):  
Ying Shen ◽  
Tao Yu ◽  
Youping Xie ◽  
Jianfeng Chen ◽  
Shih-Hsin Ho ◽  
...  

3 Biotech ◽  
2019 ◽  
Vol 9 (9) ◽  
Author(s):  
Yingying He ◽  
Zhou Zheng ◽  
Meiling An ◽  
Hao Chen ◽  
Changfeng Qu ◽  
...  

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