Effects of sodium sulfate on the freshwater microalga Chlamydomonas moewusii : implications for the optimization of algal culture media

2016 ◽  
Vol 52 (1) ◽  
pp. 75-88 ◽  
Author(s):  
Roi Mera ◽  
Enrique Torres ◽  
Julio Abalde
Keyword(s):  
Sodium Sulfate ◽  
Culture Media ◽  
Algal Culture ◽  
Chlamydomonas Moewusii

scholarly journals Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species

2021 ◽  
Vol 105 (5) ◽  
pp. 2139-2156
Author(s):  
Justine Sauvage ◽  
Gary H. Wikfors ◽  
Xiaoxu Li ◽  
Mark Gluis ◽  
Nancy Nevejan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Culture Media ◽  
Fatty Acid Content ◽  
Pluronic F127 ◽  
Algal Culture ◽  
Omega 3 ◽  
Chaetoceros Muelleri ◽  
Omega 3 Fatty Acid ◽  
Live Feed ◽  
Chaetoceros Calcitrans

Abstract The efficiency of microalgal biomass production is a determining factor for the economic competitiveness of microalgae-based industries. N-acetylcysteine (NAC) and pluronic block polymers are two compounds of interest as novel culture media constituents because of their respective protective properties against oxidative stress and shear-stress-induced cell damage. Here we quantify the effect of NAC and two pluronic (F127 and F68) culture media additives upon the culture productivity of six marine microalgal species of relevance to the aquaculture industry (four diatoms-Chaetoceros calcitrans, Chaetoceros muelleri, Skeletonema costatum, and Thalassiosira pseudonana; two haptophytes-Tisochrysis lutea and Pavlova salina). Algal culture performance in response to the addition of NAC and pluronic, singly or combined, is dosage- and species-dependent. Combined NAC and pluronic F127 algal culture media additives resulted in specific growth rate increases of 38%, 16%, and 24% for C. calcitrans, C. muelleri, and P. salina, respectively. Enhanced culture productivity for strains belonging to the genus Chaetoceros was paired with an ~27% increase in stationary-phase cell density. For some of the species examined, culture media enrichments with NAC and pluronic resulted in increased omega-3-fatty acid content of the algal biomass. Larval development (i.e., growth and survival) of the Pacific oyster (Crassostrea gigas) was not changed when fed a mixture of microalgae grown in NAC- and F127-supplemented culture medium. Based upon these results, we propose that culture media enrichment with NAC and pluronic F127 is an effective and easily adopted approach to increase algal productivity and enhance the nutritional quality of marine microalgal strains commonly cultured for live-feed applications in aquaculture. Key points • Single and combined NAC and pluronic F127 culture media supplementation significantly enhanced the productivity of Chaetoceros calcitrans and Chaetoceros muelleri cultures. • Culture media enrichments with NAC and F127 can increase omega-3-fatty acid content of algal biomass. • Microalgae grown in NAC- and pluronic F127-supplemented culture media are suitable for live-feed applications.

scholarly journals Cultivation of Microalgae Chlorella Using Wine Industry by-Products

Proceedings ◽  
2021 ◽  
Vol 66 (1) ◽  
pp. 30
Author(s):  
Lidia Martin-Gordillo ◽  
María Cuaresma ◽  
Mª Ángeles Fernández-Recamales ◽  
Ana Sayago ◽  
Carlos Vílchez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Culture Media ◽  
Sustainable Growth ◽  
Wine Industry ◽  
Added Value ◽  
Growth Media ◽  
Algal Culture ◽  
Wine Production ◽  
Dpph Method ◽  
By Products

An approach of new and sustainable uses for by-products generated in the wine production industry, one of the agro-food sectors of importance, has been studied. Wine lees, a sediment obtained in different processes of decantation of wine, have been used to produce biomass of microalgae enriched in carotenoids as high added value biomolecules. Experiments to incorporate chemical components of wine lees into microalgae biomass to understand the effect of these residues on the growth and biosynthesis of carotenoids into commercial microalgae Chlorella sorokiniana have been done. Algal culture system has been optimized and preparation of culture media have been obtained by extracting in water the soluble nutrients contained in the lees at different concentrations between 5% and 50% w/v. Optimal growth was obtained using extraction of wine residues at 5% and 10% w/v. At 10% oxidative stress, measured as carotenoids production (specially lutein) and antioxidant activity (DPPH method), was more intense than the obtained using residues at 5%. Our results show that growth in culture media prepared with wine lees extracts stimulated the antioxidant activity and the production of carotenoids in C. sorokiniana cells. Preliminary information, not only to produce sustainable growth media for biomass of microalgae enriched in high value molecules, but also to reuse nutrients contained in wine industry by-products what is of particular interest in the context of a circular economy is provided.

Volatile Organic Matter in Algal Culture Media and Sea Water

Nature ◽  
1960 ◽  
Vol 185 (4715) ◽  
pp. 761-762 ◽  
Author(s):  
F. A. J. ARMSTRONG ◽  
G. T. BOALCH
Keyword(s):  
Organic Matter ◽  
Sea Water ◽  
Culture Media ◽  
Algal Culture ◽  
Volatile Organic

A simple method for assaying extracellular hydroxyl radical activity and its application to natural and synthetic waters

2003 ◽  
Vol 60 (2) ◽  
pp. 203-213 ◽  
Author(s):  
L A Molot ◽  
S A Miller ◽  
P J Dillon ◽  
C G Trick
Keyword(s):  
Hydroxyl Radical ◽  
Natural Waters ◽  
Culture Media ◽  
Cyanobacterial Bloom ◽  
Order Rate Constant ◽  
Photochemical Oxidation ◽  
Algal Culture ◽  
Simple Method ◽  
Oxidation Rates ◽  
Lake Waters

An assay has been developed to measure extracellular hydroxyl radical (OH*) activity in algal culture media and natural waters over a 4- to 5-day period. The first-order rate constant, k, for loss of absorbance at 590 or 620 nm was determined for erioglaucine, which is sensitive to OH*, insensitive to superoxide and hydrogen peroxide, and stable in the dark and under artificial radiation (280–750 nm) and solar radiation in the absence of oxidants. Variation in irradiance was accounted for by normalizing k with k for a ferric iron reference solution with dye (k/kfe). Trends in k/kfe for streams and lakes were consistent with previous data on photochemical oxidation rates of dissolved organic matter. Values for k/kfe were similar in filtered surface waters of eutrophic Heart Lake and nearby mesotrophic Lake St. George under artificial radiation. Hence, extracellular OH* did not appear to be a direct cause of the onset of a nuisance cyanobacterial bloom in Heart Lake, nor did OH* appear related to the absence of a bloom in Lake St. George. k/kfe was two orders of magnitude higher in algal culture media supplied with 8.8 mM nitrate than in lake waters.

scholarly journals Evaluation of growth of Chlorella ellipsoidea in different culture media

2015 ◽  
Vol 4 (2) ◽  
pp. 6-10
Author(s):  
F Jahan ◽  
MS Rahman ◽  
MA Hossain
Keyword(s):  
Cell Density ◽  
Culture Media ◽  
Soil Extract ◽  
Total Alkalinity ◽  
Algal Culture ◽  
Chlorella Ellipsoidea ◽  
Maximum Cell Density ◽  
Inorganic Medium ◽  
Maximum Cell ◽  
Cell Densities

An experiment was conducted to evaluate the growth of Chlorella ellipsoidea in three different media viz,. medium I (pulse bran), medium II (soil extract) and medium III (inorganic) under the natural environmental conditions. The alga, C. ellipsoidea, reached maximum cell density of 56.32 × 106 cells ml-1 in 10 days in medium I (pulse bran), maximum cell density of 102.99 × 106 cells ml-1 in 11 days in medium II (soil extract) and maximum cell density of 64.23 × 106 cells ml-1 in 12 days in medium III (inorganic medium). The ranges of water temperature, air temperature and light intensity were 22 to 32ºC, 22 to 34ºC and 2.11 to 4.31 (× 103) lux, respectively during the culture period. The average sunshine period was 7.65 ± 1.57 hours. Total alkalinity, free CO2, pH, NO3-N, PO4- P of algal culture medium I, medium II and medium III were 220, 200 and 150 mg L-1 ; 26, 9 and 19 mg L-1; 7.9, 7.6 and 7.5; 45, 45 and 133.33 mg L-1; 10.9, 15.1 and 37.06 mg L-1, respectively. Cell densities of cultures of C. ellipsoidea under three treatments I, II and III, it can be concluded that cell densities under 3 treatments are significantly different (F=39.78) and treatment II (soil extract medium) is the best for algal (C. ellipsoidea) culture among three treatments. DOI: http://dx.doi.org/10.3329/ijarit.v4i2.22636 Int. J. Agril. Res. Innov. & Tech. 4 (2): 6-10, December, 2014

scholarly journals Culture of Chlorella ellipsoidea in different culture media

2017 ◽  
Vol 7 (1) ◽  
pp. 51-57
Author(s):  
MM Mohshina ◽  
M Shahjahan ◽  
P Chowdhury ◽  
MS Rahman
Keyword(s):  
Culture Media ◽  
Algal Cell ◽  
Algal Species ◽  
Total Alkalinity ◽  
Algal Culture ◽  
Chlorella Ellipsoidea ◽  
Maximum Cell Density ◽  
The North ◽  
Cell Densities ◽  
Powder Extract

An experiment of algal culture was conducted in natural light and temperature conditions at a balcony of a room at the 2nd floor of Fisheries Faculty Building facing the north. The experiment was done to evaluate the growth of Chlorella ellipsoidea in four different media, viz, medium I (inorganic), medium II (organic, whole pulse powder extract), medium III (organic, whole lentil powder extract) and medium IV (organic, whole gram powder extract) under natural environment conditions during January-June, 2015. Growth rates of the algal species in four different media were found not significantly different. The alga, C. ellipsoidea attained maximum cell density of 28.89×106 cell ml-1 in the 15th day in medium I, of 30.69×106 cell ml-1 in the 13th day in medium II, of 26.18×106 cell ml-1 in the 15th day in medium III and of 21.12×106 cell ml-1 in the 13th day in medium IV. The ranges of air temperature, water temperature and light intensity were 21°C to 38°C, 23°C to 36°C and 2.28×103to 9.60×103 Lux respectively during the culture period. The average sunshine period was 5.87±2.82 hrs. Total alkalinity, free CO2, pH , NO3-N and PO4-P of algal culture media I, II, III and IV were 128, 540, 554 and 322 mgL-1; 32, 162, 102, 70 mgL-1; 7.4, 8, 7.9 and 7.9; 180, 36.6, 62.4 and 150 mgL-1, and 25.2, 48.2, 42.4 and 45.6 mgL-1, respectively. According to ANOVA of cell densities of cultures of C. ellipsoidea under treatments are not significantly different (F=1.441077). It is clear that differences between them are not significant i.e. mean algal cell densities are more or less same as differences between treatments are less than 20%.Int. J. Agril. Res. Innov. & Tech. 7 (1): 51-57, June, 2017

High-pressure freezing of cells in suspension for low-voltage scanning electron microscopy (LVSEM)

1991 ◽  
Vol 49 ◽  
pp. 64-65
Author(s):  
Marek Malecki ◽  
James Pawley ◽  
Hans Ris
Keyword(s):  
Electron Microscopy ◽  
High Pressure ◽  
Low Voltage ◽  
Culture Media ◽  
Cell Structure ◽  
Freeze Substitution ◽  
Ice Crystal ◽  
High Pressure Freezing ◽  
Cell Culture Media ◽  
Voltage Scanning

The ultrastructure of cells suspended in physiological fluids or cell culture media can only be studied if the living processes are stopped while the cells remain in suspension. Attachment of living cells to carrier surfaces to facilitate further processing for electron microscopy produces a rapid reorganization of cell structure eradicating most traces of the structures present when the cells were in suspension. The structure of cells in suspension can be immobilized by either chemical fixation or, much faster, by rapid freezing (cryo-immobilization). The fixation speed is particularly important in studies of cell surface reorganization over time. High pressure freezing provides conditions where specimens up to 500μm thick can be frozen in milliseconds without ice crystal damage. This volume is sufficient for cells to remain in suspension until frozen. However, special procedures are needed to assure that the unattached cells are not lost during subsequent processing for LVSEM or HVEM using freeze-substitution or freeze drying. We recently developed such a procedure.

Kalinin: A new epithelial basement membrane adhesion molecule

1991 ◽  
Vol 49 ◽  
pp. 178-179
Author(s):  
Douglas R. Keene ◽  
Gregory P. Lunstrum ◽  
Patricia Rousselle ◽  
Robert E. Burgeson
Keyword(s):  
Basement Membrane ◽  
Culture Media ◽  
Polyacrylamide Gel Electrophoresis ◽  
Human Keratinocytes ◽  
Positive Staining ◽  
Human Amnion ◽  
Epithelial Basement Membrane ◽  
Type Vii Collagen ◽  
Keratinocyte Cell ◽  
Epithelial Basement

A mouse monoclonal antibody produced from collagenase digests of human amnion was used by LM and TEM to study the distribution and ultrastructural features of an antigen present in epithelial tissues and in cultured human keratinocytes, and by immunoaffinity chromatography to partially purify the antigen from keratinocyte cell culture media.By immunofluorescence microscopy, the antigen displays a tissue distribution similar to type VII collagen; positive staining of the epithelial basement membrane is seen in skin, oral mucosa, trachea, esophagus, cornea, amnion and lung. Images from rotary shadowed preparations isolated by affinity chromatography demonstrate a population of rod-like molecules 107 nm in length, having pronounced globular domains at each end. Polyacrylamide gel electrophoresis suggests that the size of this molecule is approximately 440kDa, and that it is composed of three nonidentical chains disulfide bonded together.

Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

1994 ◽  
Vol 52 ◽  
pp. 270-271
Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk
Keyword(s):  
Cold Storage ◽  
Structural Integrity ◽  
Culture Media ◽  
Cell Structure ◽  
Natural State ◽  
Sodium Cacodylate ◽  
Ruthenium Tetroxide ◽  
Cacodylate Buffer ◽  
Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

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