Isolation of clonal axenic strains of the symbiotic dinoflagellate Symbiodinium and their growth and host specificity1

2013 ◽  
Vol 49 (3) ◽  
pp. 447-458 ◽  
Author(s):  
Tingting Xiang ◽  
Elizabeth A. Hambleton ◽  
Jan C. DeNofrio ◽  
John R. Pringle ◽  
Arthur R. Grossman
Keyword(s):  
Symbiotic Dinoflagellate ◽  
Axenic Strains

Phylogenetic assessment of five filamentous bacteria isolated from bulking activated sludges

1998 ◽  
Vol 37 (4-5) ◽  
pp. 303-306 ◽  
Author(s):  
R. Howarth ◽  
I. M. Head ◽  
R. F. Unz
Keyword(s):  
Ribosomal Rna ◽  
Oligonucleotide Probe ◽  
Strong Relationship ◽  
Bootstrap Support ◽  
Its Sequence ◽  
Target Sequence ◽  
Common Ancestry ◽  
Filamentous Bacteria ◽  
Axenic Strains ◽  
Rrna Sequences

Nearly complete 16S ribosomal RNA (rRNA) sequences were determined for fully characterised axenic strains of Thiothrix, Eikelboom type O21N, and Eikelboom type 1701 originally isolated from bulking activated sludges. Thiothrix strains formed a monophyletic group (100% bootstrap support) with previously described Thiothrix nivea strain JP2 and Thiothrix ramosa. Eikelboom type O21N strain AP3 revealed a sufficiently strong relationship to the Thiothrix group to suggest a common ancestry for the two organism although it was not possible to designate type 021N as a species of Thiothrix. Eikelboom type 1701 contained within its sequence the target sequence of an oligonucleotide probe for the detection of Sphaerotilus natans.

scholarly journals Filling the Gaps in the Cyanobacterial Tree of Life—Metagenome Analysis of Stigonema ocellatum DSM 106950, Chlorogloea purpurea SAG 13.99 and Gomphosphaeria aponina DSM 107014

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 389
Author(s):  
Pia Marter ◽  
Sixing Huang ◽  
Henner Brinkmann ◽  
Silke Pradella ◽  
Michael Jarek ◽  
...  
Keyword(s):  
Heterotrophic Bacteria ◽  
Morphological Diversity ◽  
Cell Types ◽  
Phylogenetic Position ◽  
Metagenome Analysis ◽  
New Family ◽  
Metagenome Sequencing ◽  
Axenic Strains ◽  
Reliable Classification ◽  
Metagenomic Assembly

Cyanobacteria represent one of the most important and diverse lineages of prokaryotes with an unparalleled morphological diversity ranging from unicellular cocci and characteristic colony-formers to multicellular filamentous strains with different cell types. Sequencing of more than 1200 available reference genomes was mainly driven by their ecological relevance (Prochlorococcus, Synechococcus), toxicity (Microcystis) and the availability of axenic strains. In the current study three slowly growing non-axenic cyanobacteria with a distant phylogenetic positioning were selected for metagenome sequencing in order to (i) investigate their genomes and to (ii) uncover the diversity of associated heterotrophs. High-throughput Illumina sequencing, metagenomic assembly and binning allowed us to establish nearly complete high-quality draft genomes of all three cyanobacteria and to determine their phylogenetic position. The cyanosphere of the limnic isolates comprises up to 40 heterotrophic bacteria that likely coexisted for several decades, and it is dominated by Alphaproteobacteria and Bacteriodetes. The diagnostic marker protein RpoB ensured in combination with our novel taxonomic assessment via BLASTN-dependent text-mining a reliable classification of the metagenome assembled genomes (MAGs). The detection of one new family and more than a dozen genera of uncultivated heterotrophic bacteria illustrates that non-axenic cyanobacteria are treasure troves of hidden microbial diversity.

Amphidinolide K, a new 19-membered macrolide from the cultured symbiotic dinoflagellate Amphidinium sp

1993 ◽  
Vol 58 (24) ◽  
pp. 6928-6929 ◽  
Author(s):  
Masami Ishibashi ◽  
Masaaki Sato ◽  
Junichi Kobayashi
Keyword(s):  
Symbiotic Dinoflagellate

Factors affecting the growth and behaviour of axenic strains ofEntamoeba invadensRodhain, 1934

Parasitology ◽  
1958 ◽  
Vol 48 (3-4) ◽  
pp. 423-432 ◽  
Author(s):  
Elspeth W. McConnachie
Keyword(s):  
Heat Treatment ◽  
Liver Tissue ◽  
Optimum Concentration ◽  
Prolonged Storage ◽  
Liver Growth ◽  
Factors Affecting ◽  
Optimum Ph ◽  
Growth Promoting ◽  
Axenic Strains ◽  
Entamoeba Invadens

1. Axenic strains ofEntamoeba invadenscan be maintained indefinitely, by serial culture, in saline containing a slice of liver. Growth in this medium is poor, the amoebae are vacuolated, and few cysts are formed.2. The optimum concentration of NaCl for amoebic growth in this medium is 0·75–1·0%, and the optimum pH is 5·7–6·1.3. The source of the liver tissue in the medium is unimportant, but, of several tissues tested, liver was the only one which supported amoebic growth in serial culture.4. Suspensions of minced liver also supported amoebic growth, but the growth-promoting activity of the liver was removed completely by centrifugation, autoclaving, and Seitz- or glass-filtration. Heat treatment at 50–60° C. and prolonged storage at 8° C. partially inactivated the liver.5. Growth in axenic saline-liver medium does not affect the ability ofE. invadensto grow and undergo mass encystation when reassociated with suitable bacteria.

Cell cycle propagation is driven by light–dark stimulation in a cultured symbiotic dinoflagellate isolated from corals

Coral Reefs ◽  
2008 ◽  
Vol 27 (4) ◽  
pp. 823-835 ◽  
Author(s):  
L.-H. Wang ◽  
Y.-H. Liu ◽  
Y.-M. Ju ◽  
Y.-Y. Hsiao ◽  
L.-S. Fang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Symbiotic Dinoflagellate
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