Elongase of very long chain fatty acids 6 (ELOVL6) promotes lipid synthesis in buffalo mammary epithelial cells

2021 ◽  
Author(s):  
Xinyang Fan ◽  
Wei Zhu ◽  
Lihua Qiu ◽  
Guangle Zhang ◽  
Yongyun Zhang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Lipid Synthesis ◽  
Mammary Epithelial ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Chain Fatty Acids

scholarly journals Nutrigenomic Effect of Saturated and Unsaturated Long Chain Fatty Acids on Lipid-Related Genes in Goat Mammary Epithelial Cells: What Is the Role of PPARγ?

2019 ◽  
Vol 6 (2) ◽  
pp. 54 ◽  
Author(s):  
Einar Vargas-Bello-Pérez ◽  
Wangsheng Zhao ◽  
Massimo Bionaz ◽  
Jun Luo ◽  
Juan J. Loor
Keyword(s):  
Fatty Acids ◽  
Epithelial Cells ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Mammary Cells ◽  
Long Chain Fatty Acids ◽  
Fat Synthesis ◽  
Goat Mammary Epithelial Cells ◽  
Long Chain

A prior study in bovine mammary (MACT) cells indicated that long-chain fatty acids (LCFA) C16:0 and C18:0, but not unsaturated LCFA, control transcription of milk fat-related genes partly via the activation of peroxisome proliferator-activated receptor gamma (PPARγ). However, in that study, the activation of PPARγ by LCFA was not demonstrated but only inferred. Prior data support a lower response of PPARγ to agonists in goat mammary cells compared to bovine mammary cells. The present study aimed to examine the hypothesis that LCFA alter the mRNA abundance of lipogenic genes in goat mammary epithelial cells (GMEC) at least in part via PPARγ. Triplicate cultures of GMEC were treated with a PPARγ agonist (rosiglitazone), a PPARγ inhibitor (GW9662), several LCFA (C16:0, C18:0, t10,c12-CLA, DHA, and EPA), or a combination of GW9662 with each LCFA. Transcription of 28 genes involved in milk fat synthesis was measured using RT-qPCR. The data indicated that a few measured genes were targets of PPARγ in GMEC (SCD1, FASN, and NR1H3) while more genes required a basal activation of PPARγ to be transcribed (e.g., LPIN1, FABP3, LPL, and PPARG). Among the tested LCFA, C16:0 had the strongest effect on upregulating transcription of measured genes followed by C18:0; however, for the latter most of the effect was via the activation of PPARγ. Unsaturated LCFA downregulated transcription of measured genes, with a lesser effect by t10,c12-CLA and a stronger effect by DHA and EPA; however, a basal activation of PPARγ was essential for the effect of t10,c12-CLA while the activation of PPARγ blocked the effect of DHA. The transcriptomic effect of EPA was independent from the activation of PPARγ. Data from the present study suggest that saturated LCFA, especially C18:0, can modulate milk fat synthesis partly via PPARγ in goats. The nutrigenomic effect of C16:0 is not via PPARγ but likely via unknown transcription factor(s) while PPARγ plays an indirect role on the nutrigenomic effect of polyunsaturated LCFA (PUFA) on milk fat related genes, particularly for CLA (permitting effect) and DHA (blocking effect).

scholarly journals Medium- and Long-Chain Fatty Acids Differentially Modulate Interleukin-8 Secretion in Human Fetal Intestinal Epithelial Cells

2000 ◽  
Vol 130 (11) ◽  
pp. 2636-2640 ◽  
Author(s):  
Akira Andoh ◽  
Hiroki Takaya ◽  
Yoshio Araki ◽  
Tomoyuki Tsujikawa ◽  
Yoshihide Fujiyama ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Interleukin 8 ◽  
Long Chain Fatty Acids ◽  
Intestinal Epithelial ◽  
Long Chain ◽  
Chain Fatty Acids
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